Showing: 1 - 20 of 246 results
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Plasmid#169921PurposeDNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.DepositorInsertIRES-Puro-pEF1a-EGFP
UseCRISPRTagsExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)1x
Plasmid#112055PurposeACTB mRNA tagged with 1 copy of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation1 copy of Riboglow tag (variant A) after stop cod…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)2x
Plasmid#112056PurposeACTB mRNA tagged with 2 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation2 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)3x
Plasmid#112057PurposeACTB mRNA tagged with 3 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation3 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)4x
Plasmid#112058PurposeACTB mRNA tagged with 4 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation4 copies of Riboglow tag after stop codonPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
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pUB_smFLAG_ActB_MS2
Plasmid#81083PurposeTranslation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_ActB_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
XActB-pSP64T
Plasmid#32994DepositorInsertactivin B
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA
Plasmid#97309PurposeHR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.DepositorInsertActb HR donor
UseAAV and Mouse TargetingTagsExpressionMammalianMutationPromoterU6, EF1aAvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb HR donor
Plasmid#97317PurposeHR donor for fusing a p2A-mCherry reporter to mouse Actb.DepositorInsertActb HR donor
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-ACTB-C1
Plasmid#169796PurposeExpresses Cas9 and guide RNA for ACTB gene in humanDepositorInsertguide RNA for ACTB gene
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pAAV_ACTB CRISPIE
Plasmid#172853PurposeActb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.DepositorInsertsActb intron 1 sgRNA
DRS-2 sgRNA
CRISPIE designer exon (phase 0-0) encoding mEGFP
mRuby3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AICSDP-15:ACTB-mEGFP
Plasmid#87425PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human ACTBDepositorInsertACTB Homology Arms with mEGFP-linker (ACTB Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
actb2-lifeact-mKate2
Plasmid#109750PurposeUbiquitous zebrafish expression of mKate2 fused to the actin binding lifeact sequenceDepositorInsertmKate2 fused to the actin binding lifeact sequence
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
hACTB-RFP-Kir7.1
Plasmid#107179PurposeExpression of N-terminal fused Kir7.1 in mammalian cells.DepositorInsertKCNJ13 (KCNJ13 Human)
UseTagsRFPExpressionMammalianMutationPromoterhACTBAvailabilityAcademic Institutions and Nonprofits only -
pAAV-LACTB-dAPEX2
Plasmid#117179PurposePlasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAVTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-ACTB-mEGFP
Plasmid#115909Purposedonor to insert EGFP at the ACTB locus in human cellsDepositorInsertACTB-mEGFP (ACTB Human)
UseDonor templateTagsEGFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-ACTB-iTagRFP-T
Plasmid#115915Purposedonor to insert iTagRFP-T at the ACTB locus in human cellsDepositorInsertACTB-TagRFP-T (ACTB Human)
UseDonor templateTagsTagRFP-TExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-ACTB-iTagRFP-T
Plasmid#115916Purposedonor to insert iTagRFP-T at the ACTB locus in human cellsDepositorInsertACTB-TagRFP-T
UseDonor templateTagsTagRFP-TExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-ACTB-mEGFP
Plasmid#115917Purposedonor to insert EGFP at the ACTB locus in human cellsDepositorInsertACTB-mEGFP
UseDonor templateTagsEGFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 246 results