Showing: 21 - 40 of 718 results
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Plasmid#162459PurposeYeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
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pJYDC3
Plasmid#162460PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA designed ALFA nanobody, HA tag, HDEL sequ…ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJYDN
Plasmid#162450PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsAga2p, HA, myc tags, and eUnaG2 fluorescent prote…ExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJYDN2
Plasmid#162454PurposeYeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA nanobody and Ha and mycExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJYDN2p
Plasmid#162455PurposePositive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).DepositorInsertAga2p-DnbALFA (AGA2 )
UseShuttle vector bacteria/yeastTagsDnbALFA nanobody and HA and mycExpressionMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3
Plasmid#162456PurposeYeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJYDC1
Plasmid#162458PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the NterminusDepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsHA tag, HDEL sequence, eUnaG2 fluorescent protein…ExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJYDNgp
Plasmid#162453PurposePositive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.DepositorInsertAga2p-2Glinker-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsHA nad myc and eUnaG2 fluorescent proteinExpressionMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3p
Plasmid#162457PurposePositive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.DepositorInsertAga2p-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsALFA tag, HA and myc tags, and eUnaG2 fluorescent…ExpressionMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
C75m
Plasmid#120987PurposeMoClo golden gate assembly CD part for NahRQ-VHAC (Adam Meyer's improved NahR activator from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).DepositorInsertNahR Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
C90m
Plasmid#120998PurposeMoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer improved NahR (C75m) fused to superfolder GFP (C3m)). Please see Supplemental Documents for annotated Genbank file.DepositorInsertNahRsfGFP fusion
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKC-Tol2
Plasmid#85600PurposeSource of Tol2 transposase driven by a mini-CAGS promoter.DepositorInsertTol2 transposase
UseTagsExpressionMammalianMutationPromotermini-CAGAvailabilityAcademic Institutions and Nonprofits only -
pKUb-Tol2
Plasmid#85601PurposeSource of Tol2 transposase driven by a human ubiquitin promoter.DepositorInsertTol2 transposase
UseTagsExpressionMammalianMutationPromoterhuman UbiquitinAvailabilityAcademic Institutions and Nonprofits only -
pKTol2C-EGFP
Plasmid#85598PurposeTol2 transposon with EGFP driven by minimal-CAGs promoter.DepositorInsertTol2 transposon with EGFP
UseTagsExpressionMammalianMutationPromotermini-CAGAvailabilityAcademic Institutions and Nonprofits only -
pJA252
Plasmid#21512DepositorInsertpmex-5 (w/o ATG)
UseMultisite gatewayTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKTol2P-PTK
Plasmid#85599PurposeTol2 transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.DepositorInsertTol2 transposon with a puromycin-thymidine kinase fusion
UseTagsExpressionMammalianMutationPromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
pPTn2P-PTK
Plasmid#85604PurposePassport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.DepositorInsertPassport transposon with a puromycin-thymidine kinase fusion
UseTagsExpressionMammalianMutationPromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
pMin1
Plasmid#102959PurposeMini-transposon system suitable for sequencing, shuttle mutagenesis and gene fusionsDepositorTypeEmpty backboneUseMini-transposon system suitable for sequencing, s…TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJA304
Plasmid#28091DepositorInsert(Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR
UseTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJA245
Plasmid#21506DepositorInsertpmex-5::GFP (w introns)::(Gly)5Ala
UseMultisite gatewayTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 718 results