Showing: 41 - 56 of 56 results
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Plasmid#159659PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)
Plasmid#159660PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)
Plasmid#159661PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-1)
Plasmid#159662PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-2)
Plasmid#159663PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-GFP (gRNA: DHS-4D-3)
Plasmid#159664PurposeCRISPR/Cas9 expressing plasmid containing the gRNA targeting an enhancer of mouse Otx2.DepositorInserthSpCas9
UseCRISPRTagsExpressionMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA(hBCL2-miR9/9*-124)-iRPT
Plasmid#154881PurposeDoxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.DepositorInsertsiRFP713
Puromycin resistance gene
rtTAM2
BCL2
miR9/9*-124
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
TET1CD-CRY2-EGFP
Plasmid#82555PurposeEncodes TET1 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailabilityAcademic Institutions and Nonprofits only -
DNMT3ACD-CRY2-EGFP
Plasmid#82556PurposeEncodes DMNT3 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailabilityAcademic Institutions and Nonprofits only -
pX458-Dual-Guide-Donor-ShuttleOnly
Plasmid#171098PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationXbaI site added upstream of U6 promotor. GtoC mu…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)
Plasmid#171100PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.DepositorInserthSpCas9 (Neurog2 S. pyogenes, Mouse)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)
Plasmid#171101PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.DepositorInserthSpCas9 (Olig2 S. pyogenes, Mouse)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)
Plasmid#171102PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.DepositorInserthSpCas9 (Otx2 S. pyogenes, Mouse)
UseCRISPRTagsExpressionMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
TFORF2497
Plasmid#142115PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertASCL1 (ASCL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pCSC-LHX8-IRES-GBX1
Plasmid#182235PurposeTo convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with ASCL1, Sox11, FGF2 and two small molecules, forskolin and LDN-193189DepositorUseLentiviralTagsExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLVX-UbC-rtTA-Ngn2:2A:EGFP
Plasmid#127288Purposeall-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)DepositorInsertNgn2:2A:Ascl1 (NEUROG2 Human)
UseLentiviralTagsNgn2 and EGFP linked via 2AExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 41 - 56 of 56 results