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Showing: 21 - 40 of 50 results

  6. pADH99

    Plasmid
    #90979
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    US_HIS1
    FRT
    pENO1
    C. albicans Cas9
    pMAL2
    FLP
    NAT 1 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    90979-plasmid-map-sequence-id-180505

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  7. pADH137

    Plasmid
    #90986
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    C. albicans LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    90986-plasmid-map-sequence-id-180519

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  8. pADH140

    Plasmid
    #90988
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    C. maltosa LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    90988-plasmid-map-sequence-id-180521

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  13. pMM2_9

    Plasmid
    #183058
    Purpose
    Biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mCherry
    BM3R1-HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HAA1 and Synthetic promoter
    Availability
    Academic Institutions and Nonprofits only
    183058-plasmid-map-sequence-id-363425

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  14. pMM2_11

    Plasmid
    #183060
    Purpose
    Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus
    Depositor
    Yvonne Nygård
    Article
    Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:
    Inserts
    mCherry
    HAA1-mTurquoise2
    Use
    Synthetic Biology
    Tags
    mCherry and mTurquoise2
    Expression
    Yeast
    Mutation
    Promoter
    HAA1 and Synthetic promoter
    Availability
    Academic Institutions and Nonprofits only
    183060-plasmid-map-sequence-id-363416

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  15. pMM0638

    Plasmid
    #129005
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    129005-plasmid-map-sequence-id-261246

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  16. pMM0641

    Plasmid
    #129006
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    129006-plasmid-map-sequence-id-259445

    Enlarge

  17. pMM0646

    Plasmid
    #129007
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    129007-plasmid-map-sequence-id-259441

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  18. pMM0647

    Plasmid
    #129008
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    129008-plasmid-map-sequence-id-259450

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  19. pMM0648

    Plasmid
    #129009
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3 homology-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-homology KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    129009-plasmid-map-sequence-id-259442

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  20. pMM0649

    Plasmid
    #129010
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    129010-plasmid-map-sequence-id-270698

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Showing: 21 - 40 of 50 results