Showing: 1 - 14 of 14 results
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Plasmid#110014PurposeProtein expression and purification of GNAI3_G-alphaDepositorInsertGNAI3_G-alpha (GNAI3 Human)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204341PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO
Plasmid#204343PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-i3/Gbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai3-RLuc8
Plasmid#140975PurposeEncodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai3-RLuc8 (GNAI3 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI3
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3 ONE-GO
Plasmid#189737PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1.Gαi3-LgB91
Plasmid#134359PurposeNanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.DepositorInsertGαi3-LgB91 (GNAI3 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SV40-Galpha-i3 ONE-GO
Plasmid#204329PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-i3 ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-i3 ONE-GO
Plasmid#204334PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-i3 ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3 G183S ONE-GO
Plasmid#204338PurposeGPCR/G protein BRET biosensorDepositorInsertGalpha-i3 G183S ONE-GO
UseLentiviralTagsExpressionMutationG183S mutation in Galpha-i3 (renders it RGS insen…PromoterAvailabilityAcademic Institutions and Nonprofits only -
Gi3-CASE
Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1PromoterAvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gai3
Plasmid#196050PurposeEncodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorInsertsGAlphai3-RLuc8
GGamma9-GFP2
GBeta3
UseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 14 of 14 results