Showing: 1 - 14 of 14 results
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Plasmid#110013PurposeProtein expression and purification of GNAO1_G-alphaDepositorInsertGNAO1_G-alpha (GNAO1 Human)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Go1-CASE
Plasmid#168123PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at L91/G92 within GNAO1PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoA-RLuc8
Plasmid#140976PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaoA-RLuc8 (GNAO1 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaoB-RLuc8
Plasmid#140977PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaoB-Rluc8 (GNAO1 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaoA
Plasmid#196051PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensorDepositorInsertsGAlphaoA-RLuc8
GGamma8-GFP2
GBeta3
UseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaoB
Plasmid#196052PurposeEncodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensorDepositorInsertsGAlphaoB-RLuc8
GGamma8-GFP2
GBeta3
UseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pISH-Galpha-o
Plasmid#15930DepositorInsertGalpha-o (Gnao1 Mouse)
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
alpha 0-CFP in pdest12.2
Plasmid#36312DepositorInsertG protein alpha 0 (Gnao1 Rat)
UseTagsCFPExpressionMammalianMutationIntroduced KpnI and SpeI sites between 92nd and 9…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
alpha-0 CFP in pcDNA3.1
Plasmid#36396DepositorInsertG protein alpha 0 (Gnao1 Rat)
UseTagsCFPExpressionMammalianMutationCFP inserted into the KpnI-SpeI sites engineered …PromotercmvAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Alpha-0 Dronpa
Plasmid#44193DepositorInsertG protein alpha 0 (Gnao1 Rat)
UseTagsDronpaExpressionMammalianMutationIntroduced KpnI and SpeI sites between 92nd and 9…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-o-R179C-EE
Plasmid#55780PurposeThis is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.DepositorInsertG protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)
UseTagsEE epitope was introduced internally with D167E a…ExpressionBacterial and MammalianMutationArginine 179 was replaced with cysteinePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oA ONE-GO
Plasmid#189732PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationI250 (silent mutation in YFP)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oB ONE-GO
Plasmid#189733PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-o-YFP
Plasmid#55776PurposeThis G protein alpha-o construct contains internal insertions of YFP and the EE epitopeDepositorInsertG protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
UseTagsEE epitope was introduced internally with D167E a…ExpressionMammalianMutationA BglII site was removed from the 5' untrans…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 14 of 14 results