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Showing: 61 - 77 of 77 results

  3. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

    Plasmid
    #140196
    Purpose
    Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dSpCas9-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta) , NLS
    Expression
    Mutation
    Promoter
    PgpdA and PtrpC
    Availability
    Academic Institutions and Nonprofits only
    140196-plasmid-map-sequence-id-309706

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  4. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid
    #140193
    Purpose
    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta), NLS
    Expression
    Mutation
    D832A DNAse deactivated
    Promoter
    PgpdA and PtrpC
    Availability
    Academic Institutions and Nonprofits only
    140193-plasmid-map-sequence-id-309648

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  5. pCB1532H-RFP

    Plasmid
    #101855
    Purpose
    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
    Depositor
    Sophien Kamoun
    Article
    Pennington et al Figshare (2017)
    Type
    Empty backbone
    Use
    Fungal expression plasmid, tested in magnaporthe
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    101855-plasmid-map-sequence-id-195301

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  6. pCB1532B-RFP

    Plasmid
    #101854
    Purpose
    Golden-Gate compatible fungal transformation vector carrying BASTA (Bialaphos/ Phosphinothricin) Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
    Depositor
    Sophien Kamoun
    Article
    Pennington et al Figshare (2017)
    Type
    Empty backbone
    Use
    Fungal expression plasmid, tested in magnaporthe
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    101854-plasmid-map-sequence-id-204750

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  7. pCB1532S-RFP

    Plasmid
    #101856
    Purpose
    Golden-Gate compatible fungal transformation vector carrying Sulfonylurea Resistance (Chlorimuron Ethyl) with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
    Depositor
    Sophien Kamoun
    Article
    Pennington et al Figshare (2017)
    Type
    Empty backbone
    Use
    Fungal expression plasmid, tested in magnaporthe
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    101856-plasmid-map-sequence-id-197099

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Showing: 61 - 77 of 77 results