Addgene

Showing: 41 - 60 of 146 results

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pAAV-CAG-DIO(ChRger2-TS-YFP)

Plasmid

#127239

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger2-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO(ChRger3-TS-YFP)

Plasmid

#127242

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger3-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO(ChRger1-TS-YFP)

Plasmid

#127245

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger1-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-(STChRger2-TS-EYFP)

Plasmid

#129394

Purpose

Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

soma targeted ChRger2

Use

AAV and Cre/Lox

Tags

Kv2.1-TS-EYFP

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pET32a-hNRG1a

Plasmid

#199235

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Mol Biol Rep. 2022 Dec;49(12):11715-1172

Insert

Neuregulin (NRG1 Human)

Use

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC48A1

Plasmid

#132156

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC48A1 (SLC48A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC48A1_STOP

Plasmid

#161322

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC48A1 (SLC48A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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pTol2CG NBT:NRGIIIa:polyA

Plasmid

#193012

Purpose

Tol2 transgenic construct that will express human Neuregulin1 Type IIIa in neurons

Depositor

William Talbot

Article

Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

Inserts

NBT
NRG1 type IIIa (NRG1 Human)

Use

Transposon transgenesis

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTol2CG NBT:NRGIIIa_R551Q:polyA

Plasmid

#193013

Purpose

Tol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neurons

Depositor

William Talbot

Article

Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

Inserts

NBT
NRG1 type IIIa R551Q (NRG1 Human)

Use

Transposon transgenesis

Tags

Expression

Mammalian

Mutation

ENST00000287842, NM_013956, c.1652G>A, p.(Arg5…

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-shMcu-2

Plasmid

#181867

Purpose

Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

Depositor

Hilmar Bading

Article

Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

Insert

mitochondrial calcium uniporter (Mcu Mouse)

Use

AAV, Adenoviral, and Mouse Targeting

Tags

hrGFP

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pAAV-shMcu-1

Plasmid

#181868

Purpose

Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter

Depositor

Hilmar Bading

Article

Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

Insert

mitochondrial calcium uniporter (Mcu Mouse)

Use

AAV, Adenoviral, and Mouse Targeting

Tags

hrGFP

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NRG1-COMP5AP-AviTag-9xHis

Plasmid

#157230

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NRG1 (NRG1 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NRG1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156666

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NRG1 (NRG1 Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Availability

Academic Institutions and Nonprofits only

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Neuregulin-CRD (Cysteine-rich domain, Type III) scFv [N126B/31]

Plasmid

#190528

Purpose

Mammalian Expression of Neuregulin-CRD (Cysteine-rich domain, Type III) scFV. Derived from hybridoma N126B/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Neuregulin-CRD (Cysteine-rich domain, Type III) (Homo sapiens) recombinant scFV (NRG1 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Anti-Neuregulin-HBD (Heparin binding domain, Type I/II) [N120A/9.1R]

Plasmid

#114507

Purpose

Mammalian Expression Plasmid of anti-Neuregulin-HBD (Heparin binding domain, Type I/II) (Human). Derived from hybridoma N120A/9.1.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuregulin-HBD (Heparin binding domain, Type I/II) (Homo sapiens) recombinant mouse monoclonal antibody (NRG1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Availability

Academic Institutions and Nonprofits only

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Barkor Δ10

Plasmid

#30500

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 Apr 25. (

Insert

Barkor (ATG14 Human)

Use

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

1-483aa

Promoter

Availability

Academic Institutions and Nonprofits only
CCD+BATS

Plasmid

#30499

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 Apr 25. (

Insert

Barkor (ATG14 Human)

Use

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

71-180aa (CCD) + 413-492aa (BATS)

Promoter

Availability

Academic Institutions and Nonprofits only
Beclin-BATS

Plasmid

#30498

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 Apr 25. (

Insert

Beclin, Barkor (BECN1, ATG14 Human)

Use

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

Beclin 1 + 413-492 aa of Barkor

Promoter

Availability

Academic Institutions and Nonprofits only
pEF1a-Foxd3-ER

Plasmid

#37270

Depositor

Patricia Labosky

Insert

Foxd3 (Foxd3 Mouse)

Use

Tags

ER

Expression

Mammalian

Mutation

Promoter

EF1

Availability

Academic Institutions and Nonprofits only
MB121 (Nf2 BBA)

Plasmid

#12170

Depositor

Tyler Jacks

Insert

Nf2 BBA (Nf2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

BBA: dominant negative Nf2, 7 Blue Box residues c…

Promoter

Availability

Academic Institutions and Nonprofits only