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Showing: 41 - 60 of 5712 results

  5. TKIT NFL gRNA1 gRNA2

    Plasmid
    #182681
    Purpose
    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Availability
    Academic Institutions and Nonprofits only
    182681-plasmid-map-sequence-id-363020

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  6. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

    Plasmid
    #217340
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Availability
    Academic Institutions and Nonprofits only
    217340-plasmid-map-sequence-id-426478

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  10. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182682
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NA
    Availability
    Academic Institutions and Nonprofits only
    182682-plasmid-map-sequence-id-363082

    Enlarge

  11. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182685
    Purpose
    Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and NA
    Availability
    Academic Institutions and Nonprofits only
    182685-plasmid-map-sequence-id-363019

    Enlarge

  12. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor

    Plasmid
    #182686
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    NA
    Availability
    Academic Institutions and Nonprofits only
    182686-plasmid-map-sequence-id-363076

    Enlarge

  13. pPD132.49

    Plasmid
    #1724
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  14. pPD157.63

    Plasmid
    #1710
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    vit2tsa, T20G511N, T20G511C, let8583', decoy
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    1710-plasmid-map-sequence-id-316

    Enlarge

  15. pPD138.11

    Plasmid
    #1721
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    EGFPN, EGFPC, IVSL, myo3-2341, myo3Tsa
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    1721-plasmid-map-sequence-id-327

    Enlarge

  16. pPD107.09

    Plasmid
    #1703
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SupTrp5', SupTrp3', T7p, lacZN, OriF1&g…
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    1703-plasmid-map-sequence-id-309

    Enlarge

  17. pPD158.117

    Plasmid
    #1711
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    unc119tsa, RFPN, RFPivs2, RFPV105A, RFPivs4, RFPS…
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    1711-plasmid-map-sequence-id-317

    Enlarge

  18. pPD152.79

    Plasmid
    #1704
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    dpy30Tsa, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, …
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    1704-plasmid-map-sequence-id-310

    Enlarge

  19. pPD159.88

    Plasmid
    #1707
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    rol6tsa, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, I…
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    1707-plasmid-map-sequence-id-313

    Enlarge

  20. pPD187.49

    Plasmid
    #1722
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    T7p, SPGFPN, SPGFPC, Sp6p, OriF1>>, OriF1&l…
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    1722-plasmid-map-sequence-id-328

    Enlarge

Showing: 41 - 60 of 5712 results