Showing: 1 - 20 of 62 results
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Plasmid#31589DepositorInsertmicrotubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_MAPRE1-Halo
Plasmid#175345PurposeProduction of HaloTag fusion proteinDepositorInsertMAPRE1 (MAPRE1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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MAPRE1 F9.3 gRNA
Plasmid#90746Purpose3rd generation lentiviral gRNA plasmid targeting human MAPRE1DepositorInsertMAPRE1 (Guide Designation F9.3)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
MAPRE1 F10.3 gRNA
Plasmid#90747Purpose3rd generation lentiviral gRNA plasmid targeting human MAPRE1DepositorInsertMAPRE1 (Guide Designation F10.3)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1
Plasmid#107726PurposeKnock out of EB1 in human cells by CRISPR/Cas9DepositorInsertMAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2
Plasmid#107727PurposeKnock out of EB1 in human cells by CRISPR/Cas9DepositorInsertMAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3
Plasmid#107728PurposeKnock out of EB1 in human cells by CRISPR/Cas9DepositorInsertMAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1
Plasmid#197424Purposeπ-element knock-in into exon 5 of MAPRE1DepositorInsertMAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2
Plasmid#197425Purposeπ-element knock-in into exon 5 of MAPRE1DepositorInsertMAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3
Plasmid#197426Purposeπ-element knock-in into exon 5 of MAPRE1DepositorInsertMAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-MAPRE1
Plasmid#207794PurposeDonor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793DepositorArticleInsertMAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
human EB1 in pBluescript II
Plasmid#39324DepositorInsertEB1 (MAPRE1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
human EB1 in PET28a for protein expression
Plasmid#39297DepositorInsertEB1 (MAPRE1 Human)
UseTags6HISExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
human EB1 fused to monomeric RFP
Plasmid#39323DepositorInsertsUseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
human EB1 in pEGFP N1 to express EB1-GFP (JB131)
Plasmid#39299DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_IRES-mCherry
Plasmid#197427PurposeHomology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5DepositorInsertIRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized Y44 Q46 both converted to A (JB140)
Plasmid#39301DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationY44A and Q46APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
human EB1-GFP mutagenized K148 K150 K151 all converted to A (JB144)
Plasmid#39305DepositorInsertEB1 (MAPRE1 Human)
UseTagsEGFPExpressionMammalianMutationK148A, K150A and K151APromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 62 results