Addgene

Showing: 21 - 40 of 77 results

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pRMC2-tat:msfGFP

Plasmid

#194915

Purpose

Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

Depositor

Rikke Meyer

Article

Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

Insert

Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP

Use

Tetracycline-inducible expression vector for stap…

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MC2R-DuET

Plasmid

#213336

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

MC2R (MC2R Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Availability

Academic Institutions and Nonprofits only

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PGRMC2-CD4d3+4-bio

Plasmid

#73112

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding PGRMC2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

PGRMC2 (PGRMC2 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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SMC2 H5.1 gRNA

Plasmid

#90892

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC2 (Guide Designation H5.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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SMC2 H6.1 gRNA

Plasmid

#90893

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC2 (Guide Designation H6.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Mc2r-mod

Plasmid

#48173

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of MC2R (MC2R Human)

Use

Luciferase

Tags

Expression

Mutation

A/G MUTATION IN ATG OF UORF

Promoter

Availability

Academic Institutions and Nonprofits only
pMC234r-ccdB/TPK2

Plasmid

#176179

Purpose

yeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

dropout cassette containing toxic genes ccdB and TPK2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC2

Plasmid

#173468

Purpose

Human PGRMC2 containing an N-terminal FLAG tag

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC2 (PGRMC2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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mc2 neo EF1a

Plasmid

#206220

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 puro EF1a

Plasmid

#206221

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 hygro EF1a

Plasmid

#206222

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 blast EF1a

Plasmid

#206223

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 mNeon EF1a

Plasmid

#206224

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 mCherry EF1a

Plasmid

#206225

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 neo hPGK

Plasmid

#206226

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 puro hPGK

Plasmid

#206227

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 hygro hPGK

Plasmid

#206228

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 blast hPGK

Plasmid

#206229

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 mNeon hPGK

Plasmid

#206230

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
mc2 mCherry hPGK

Plasmid

#206231

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Brett Stringer, Simon Conn

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge