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Showing: 21 - 40 of 68 results

  1. pMT493

    Plasmid
    #139398
    Purpose
    Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Availability
    Academic Institutions and Nonprofits only
    139398-plasmid-map-sequence-id-313939

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  2. pMT494

    Plasmid
    #139399
    Purpose
    Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Availability
    Academic Institutions and Nonprofits only
    139399-plasmid-map-sequence-id-313941

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  3. MT4_C30Y R31W

    Plasmid
    #105712
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 3 subisoform with C30Y and R31W substitution
    Depositor
    Artur Krężel
    Insert
    Metallothionein 4 (MT4 Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    This subisoform has a C30Y and R31W substitution
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    105712-plasmid-map-sequence-id-203330

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  5. CMV-mt4xi-GCaMP6f

    Plasmid
    #192075
    Purpose
    Fluorescent reporter for mitochondrial calcium
    Depositor
    Timothy Ryan
    Insert
    4 repeats of improved COX8 signal peptide plus GCaMP6f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    192075-plasmid-map-sequence-id-378919

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  9. DOT1L

    Plasmid
    #40736
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  12. pAH237

    Plasmid
    #121146
    Purpose
    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast
    Depositor
    Katsunori Tanaka
    Article
    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.
    Inserts
    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    Use
    CRISPR
    Tags
    Flag
    Expression
    Yeast
    Mutation
    Promoter
    S.pombe rrk1 and nmt41
    Availability
    Academic Institutions and Nonprofits only
    121146-plasmid-map-sequence-id-234796

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  14. pAH243

    Plasmid
    #121147
    Purpose
    Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast
    Depositor
    Katsunori Tanaka
    Article
    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.
    Inserts
    humanized Streptococcus pyogenes Cas9
    gRNA expression module
    Use
    CRISPR
    Tags
    Flag
    Expression
    Yeast
    Mutation
    Promoter
    S.pombe rrk1 and nmt41
    Availability
    Academic Institutions and Nonprofits only
    121147-plasmid-map-sequence-id-234831

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  15. pREP41-MCS+

    Plasmid
    #52690
    Purpose
    Same as pREP41, with better MCS
    Depositor
    Michael Nick Boddy
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pnmt41
    Availability
    Academic Institutions and Nonprofits only
    52690-plasmid-map-sequence-id-82000

    Enlarge

  16. pREP42-MCS+

    Plasmid
    #52691
    Purpose
    Same as pREP42, with better MCS
    Depositor
    Michael Nick Boddy
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pnmt41
    Availability
    Academic Institutions and Nonprofits only
    52691-plasmid-map-sequence-id-210876

    Enlarge

  17. LjUBQ:MtPIP7:T35s

    Plasmid
    #164766
    Purpose
    Overexpression of Peptide coding gene (Binary vector)
    Depositor
    Wolf Scheible
    Insert
    MT4Noble_020581.1
    Use
    Tags
    Terminator 35S
    Expression
    Plant
    Mutation
    Promoter
    Lotus japonicus Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    164766-plasmid-map-sequence-id-325452

    Enlarge

  18. LjUBQ:MtMEG2:T35s

    Plasmid
    #164754
    Purpose
    Overexpression of Peptide coding gene (Binary vector)
    Depositor
    Wolf Scheible
    Insert
    MT4Noble_004794.1
    Use
    Tags
    Terminator 35S
    Expression
    Plant
    Mutation
    Promoter
    Lotus japonicus Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    164754-plasmid-map-sequence-id-325450

    Enlarge

  20. 6CKO (DOT1L)

    Plasmid
    #119962
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Use
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    119962-plasmid-map-sequence-id-234358

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Showing: 21 - 40 of 68 results