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Showing: 41 - 60 of 65 results

  5. PB-TO-oNGN2

    Plasmid
    #198397
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    rtTA3G
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…
    Promoter
    CAG, EF1a, and TRE3G
    Availability
    Academic Institutions and Nonprofits only
    198397-plasmid-map-sequence-id-389535

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  7. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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  8. jk95

    Plasmid
    #120176
    Purpose
    CAG:gfp:P2A:mycNRNX
    Depositor
    Anthony Zador
    Insert
    CAG:gfp:P2A:myc_NRXN
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120176-plasmid-map-sequence-id-227831

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  9. pNS35 (multiAsCas12a piggyBac)

    Plasmid
    #217336
    Purpose
    piggyBac expression of multiAsCas12a
    Depositor
    Luke Gilbert
    Insert
    multiAsCas12a
    Use
    CRISPR
    Tags
    6xMycNLS and HA-SV40NLS-P2A-TagBFP2
    Expression
    Mammalian
    Mutation
    R1226A/E174R/S542R/K548R
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    217336-plasmid-map-sequence-id-426480

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  10. pCH45 (multiAsCas12a-KRAB lenti)

    Plasmid
    #217330
    Purpose
    Lentiviral expression of multiAsCas12a-KRAB
    Depositor
    Luke Gilbert
    Insert
    multiAsCas12a
    Use
    CRISPR and Lentiviral
    Tags
    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2
    Expression
    Mutation
    R1226A/E174R/S542R/K548R
    Promoter
    UCOE-SFFV
    Availability
    Academic Institutions and Nonprofits only
    217330-plasmid-map-sequence-id-426762

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  11. pCH4 (denAsCas12a-KRAB lenti)

    Plasmid
    #217331
    Purpose
    Lentiviral expression of denAsCas12a-KRAB
    Depositor
    Luke Gilbert
    Insert
    denAsCas12a
    Use
    CRISPR and Lentiviral
    Tags
    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2
    Expression
    Mutation
    D908A/E174R/S542R/K548R
    Promoter
    UCOE-SFFV
    Availability
    Academic Institutions and Nonprofits only
    217331-plasmid-map-sequence-id-426761

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  12. pCH61 (multiAsCas12a-KRAB piggyBac)

    Plasmid
    #217332
    Purpose
    piggyBac expression of multiAsCas12a-KRAB
    Depositor
    Luke Gilbert
    Insert
    multiAsCas12a
    Use
    CRISPR
    Tags
    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2
    Expression
    Mammalian
    Mutation
    R1226A/E174R/S542R/K548R
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    217332-plasmid-map-sequence-id-426476

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  13. pCH62 (denAsCas12a-KRAB piggyBac)

    Plasmid
    #217333
    Purpose
    piggyBac expression of denAsCas12a-KRAB
    Depositor
    Luke Gilbert
    Insert
    denAsCas12a
    Use
    CRISPR
    Tags
    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2
    Expression
    Mammalian
    Mutation
    D908A/E174R/S542R/K548R
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    217333-plasmid-map-sequence-id-426481

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  14. pNS49 (enAsCas12a-KRAB piggyBac)

    Plasmid
    #217335
    Purpose
    piggyBac expresion of enAsCas12a-KRAB
    Depositor
    Luke Gilbert
    Insert
    enAsCas12a
    Use
    CRISPR
    Tags
    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2
    Expression
    Mammalian
    Mutation
    E174R/S542R/K548R
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    217335-plasmid-map-sequence-id-426493

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Showing: 41 - 60 of 65 results