We narrowed to 57 results for: pcold

1. pCold-TF-hPLEKHM1

   Plasmid

   #64146

   Purpose

   For protein expression in E.coli

   Depositor

   Tamotsu Yoshimori

   Article

   Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

   Insert

   PLEKHM1 (PLEKHM1 Human)
   

   Use

   Tags

   6xHis-tag and Trigger Factor (TF)

   Expression

   Bacterial

   Mutation

   Promoter

   cspA

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCold-I-Dsup

   Plasmid

   #90021

   Purpose

   To express His-tagged Dsup in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   cspA

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCold TDP43 WT

   Plasmid

   #27451

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Factor Xa, GST, and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pColdI-DDX3_helicase_core

   Plasmid

   #233618

   Purpose

   Expression of His-tagged helicase core region of DDX3X in E.coli

   Depositor

   Shintaro Iwasaki

   Article

   Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

   Insert

   DDX3X helicase core WT (DDX3X Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCold-TF-hRubicon

   Plasmid

   #64147

   Purpose

   For protein expression in E. coli

   Depositor

   Tamotsu Yoshimori

   Article

   Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

   Insert

   Rubicon (RUBCN Human)
   

   Use

   Tags

   6xHis-tag and Trigger Factor (TF)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCold I-Hero11

   Plasmid

   #187928

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero11

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero11 (C19orf53 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCold I-Hero7

   Plasmid

   #187926

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero7

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero7 (SERF2 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCold I-Hero9

   Plasmid

   #187927

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero9

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero9
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCold I-Hero13

   Plasmid

   #187929

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero13

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero13 (BEX3 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCold I-Hero20

    Plasmid

    #187930

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero20

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero20 (C11orf58 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCold I-Hero45

    Plasmid

    #187931

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero45

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero45 (SERBP1 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pColdI-DDX3_helicase_core_Q360L

    Plasmid

    #233619

    Purpose

    Expression of His-tagged helicase core region of DDX3X with Q360L mutation in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    DDX3X helicase core Gln360Leu (DDX3X Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Gln360Leu

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pColdI-DDX3_helicase_core_Q360P

    Plasmid

    #233620

    Purpose

    Expression of His-tagged helicase core region of DDX3X with Q360P mutation in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    DDX3X helicase core Gln360Pro (DDX3X Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Gln360Pro

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pColdI-SBP-eIF4A1

    Plasmid

    #233621

    Purpose

    Expression of SBP-tagged eIF4A1 in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    SBP-eIF4A1 (EIF4A1 Human)
    

    Use

    Tags

    His6-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCold CL7-Cas9

    Plasmid

    #124890

    Purpose

    obtaining full Cas9 RNP

    Depositor

    Yi Liu

    Article

    Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCold TDP43 Q331K

    Plasmid

    #27454

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCold TDP43 M337V

    Plasmid

    #27453

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Methionine 337 mutated to Valine (M337V)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pCold CL7-Cas12a

    Plasmid

    #124891

    Purpose

    obtaining full Cas12a RNP

    Depositor

    Yi Liu

    Article

    Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

    Insert

    Cas12a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pColdI-GST-mGTSF1

    Plasmid

    #214671

    Purpose

    Bacterial expression of GST-tagged mouse GTSF1

    Depositor

    Phillip Zamore

    Article

    Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

    Insert

    GTSF1 (Gtsf1 Mouse)
    

    Use

    Tags

    6x His and GST

    Expression

    Bacterial

    Mutation

    Promoter

    CspA

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCold TDP43 G294A

    Plasmid

    #27452

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Glycine 294 mutated to Alanine (G294A)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only