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Addgene
Showing: 1 - 19 of 19 results
  1. rFIXX-bp chain A (IRESneo)

    Plasmid
    #82450
    Purpose
    Chain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.
    Depositor
    Insert
    rFIXX-bp chain A
    Use
    Tags
    Polyhistidine
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pBTSG

    Plasmid
    #159420
    Purpose
    Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells
    Depositor
    Insert
    thermostable GFP (TGP)
    Use
    Tags
    polyhistidine (poly-His)
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pBP-HexHis

    Plasmid
    #72983
    Purpose
    Hexahistidine N-terminal tag (5' TAAA/3' CATA fusion)
    Depositor
    Insert
    Polyhistidine (HHHHHH) N-terminal tag
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pET26b(+)-ATI_QconCAT_1

    Plasmid
    #163957
    Purpose
    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.
    Depositor
    Insert
    ATI QconCAT
    Use
    Tags
    polyhistidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pTW5126

    Plasmid
    #129732
    Purpose
    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein
    Depositor
    Insert
    eatA-DUF::His8
    Use
    Tags
    9His tag
    Expression
    Bacterial
    Mutation
    replaces the domain of unknown function correspon…
    Promoter
    araBAD
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 19 of 19 results