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pLentiCRISPRv1_SDHB

Plasmid

#177981

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

Depositor

Jessica Spinelli, David Sabatini

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting SDHB (SDHB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_SDHB_WT_V5

Plasmid

#83005

Purpose

Gateway Donor vector containing SDHB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SDHB (SDHB Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLYS5-SDHB-Flag

Plasmid

#50055

Purpose

Expression of human SDHB in mammalian cells

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

SDHB (SDHB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pSEMS-SDHB-Halo7Tag

Plasmid

#108973

Purpose

Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)

Depositor

Karin Busch

Article

Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

Insert

succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)

Use

Tags

Halo7-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1 tTA SDHB

Plasmid

#177977

Purpose

All-in-one dox-repressible expression of cDNA

Depositor

Jessica Spinelli, David Sabatini

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

SDHB (SDHB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Silent point mutations to SDHB sgRNA target

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172670

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

SDHB pegRNA and SDHB_nick-sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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TAL2222

Plasmid

#36681

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

SDHB-TALEN_Left (sdhB Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2223

Plasmid

#36682

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

SDHB-TALEN_Right (sdhB Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pSEZ-1

Plasmid

#51868

Purpose

Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

Depositor

Meng-Qiu Dong

Article

Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

Insert

psdhb-1::mtLS-cpYFP

Use

Tags

Expression

Worm

Mutation

Promoter

sdhb-1

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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