Showing: 1 - 20 of 25 results
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Plasmid#109816PurposeProtein expression and purification of SPRED1_WH1DepositorInsertSPRED1_WH1 (SPRED1 Human)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:SPRED1
Plasmid#118851PurposeExpresses human SPRED1 and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertSPRED1 (SPRED1 Zebrafish, Human)
UseZebrafish expression, tol7TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
R777-E321 Hs.SPRED1
Plasmid#70605PurposeGateway ORF clone of human SPRED1 [NM_152594.2] with stop codon (for native or N-terminal fusions)DepositorInsertSPRED1 (SPRED1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA spred1, mitfa:Cas9
Plasmid#118843PurposeTargets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertspred1 gRNA (spred1 Zebrafish)
UseCRISPR; Tol2TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
R777-E322 Hs.SPRED1-nostop
Plasmid#70606PurposeGateway ORF clone of human SPRED1 [NM_152594.2] without stop codon (for C-terminal fusions)DepositorInsertSPRED1 (SPRED1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)
Plasmid#62577PurposeTranslational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding siteDepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)
Plasmid#62579PurposeTranslational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)
Plasmid#62580PurposeTranslational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseTagsExpressionMutationmiR-21 binding site 1 mutant (TAAGCTA --> TAGA…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)
Plasmid#62581PurposeTranslational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.DepositorInsertSPRED1 (SPRED1 Human)
UseLuciferaseTagsExpressionMutationmiR-21 binding site 2 mutant (TAAGCTA --> TAGA…PromoterAvailabilityAcademic Institutions and Nonprofits only -
R725-X09-635: His6-tev-Hs.SPRED1(13-125)
Plasmid#159581PurposeBaculovirus protein expression of His6-tev-Hs.SPRED1(13-125)DepositorInsertHis6-tev-Hs.SPRED1(13-125) (SPRED1 Human)
UseTagsHis6-tevExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDest-635
Plasmid#159582PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneUseTagsHis6ExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)
Plasmid#159579PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)DepositorInsertHis6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)
Plasmid#159580PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)DepositorInsertHis6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA, mitfa:Cas9
Plasmid#118840PurposeTargets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorTypeEmpty backboneUseCRISPR; Tol2TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR 2xU6:gRNA, mitfa:Cas9
Plasmid#118844PurposeTargets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorTypeEmpty backboneUseCRISPR; Tol2TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:EGFP
Plasmid#118850PurposeExpresses EGFP and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertmitfa: EGFP (mitfa Zebrafish, Synthetic)
UseZebrafish expression, tol6TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9
Plasmid#118842PurposeTargets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertcdkn2a gRNA (LOC100329528 Zebrafish)
UseCRISPR; Tol2TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:BRAF
Plasmid#118846PurposeExpresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertBRAF V600E (BRAF Zebrafish, Human)
UseZebrafish expression, tol2TagsExpressionMutationBRAF V600EPromoterAvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:NRAS
Plasmid#118847PurposeExpresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertNRAS Q61R (NRAS Zebrafish, Human)
UseZebrafish expression, tol3TagsExpressionMutationNRAS Q61RPromoterAvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:KIT L576P
Plasmid#118848PurposeExpresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertKIT L576P (KIT Zebrafish, Human)
UseZebrafish expression, tol4TagsExpressionMutationKIT L576PPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 25 results