Showing: 1 - 20 of 24 results
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pmiRGLO-UBE2I-202
Plasmid#117377PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR UBE2I-202
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1AvailabilityAcademic Institutions and Nonprofits only -
pET23a-mUbc9
Plasmid#53137PurposeE.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)DepositorInsertUbc9 (Ube2i Mouse)
UseTagsuntaggedExpressionBacterialMutationprotein sequence is identical to humanPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
mCherry-E2
Plasmid#175242PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylationDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-E2
Plasmid#175243PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to YpetDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
CyPet-FKBP-E2
Plasmid#175244PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycinDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
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p3991 pET ubcH9
Plasmid#8651DepositorInsertUbcH9 (UBE2I Human)
UseTagsHAExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/UBC9
Plasmid#20082DepositorInsertUBC9 (UBE2I Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p3258 pCMV hUBC9 wt HA
Plasmid#14438DepositorInsertUBC9 (UBE2I Human)
UseTagsHAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p3259 pCMV hUBC9 mt HA
Plasmid#14439DepositorInsertUBC9 C93S (UBE2I Human)
UseTagsHAExpressionMammalianMutationCys93Ser mutation (TGC changed to AGC)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSC-PreE2
Plasmid#52264PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linkerDepositorInsertUbc9 GST fusion (UBE2I Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSC-IntE2
Plasmid#52265PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linkerDepositorInsertUbc9 GST fusion (UBE2I Human, Synthetic)
UseTagsGST and GyrA-inteinExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9
Plasmid#133909PurposeBacterial expression of His6-Ubc9DepositorInsertSUMO-conjugating enzyme UBC9
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
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pET28-His6-Ubc9-C93A
Plasmid#133910PurposeBacterial expression of His6-Ubc9-C93ADepositorInsertSUMO-conjugating enzyme UBC9
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 93 to AlaninePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-C138A
Plasmid#133911PurposeBacterial expression of His6-Ubc9-C138ADepositorInsertSUMO-conjugating enzyme UBC9
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationCysteine 138 to AlaninePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-K14R
Plasmid#185759PurposeBacterial expression of His6-Ubc9-K14RDepositorInsertSUMO-conjugating enzyme UBC9
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationK14RPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pSUMO1
Plasmid#52258PurposeBacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only -
pSUMO2
Plasmid#52259PurposeBacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 24 results