We narrowed to 658 results for: CHI;

...co-founder of BGI-China presenting, it’s no wonder it was attended by over 300 Chinese scientists eager...Having never been to China myself, I was pleased to be able to participate when the organizers of the...Dmitry Samarsky, and a very welcoming team from China’s Ribobio, the illustrious speaker list attracted...were potential Addgene requestors and depositors. China is in one of the top 10 countries in number of plasmid...where one can shop for components of traditional Chinese medicine. There were hundreds of stalls selling...replication of RNA in the absence of evolved enzymatic machinery (i.e. RNA replication with no proteins). The chemistry... china...

...successful ChIP experiment! Image courtesy of https://www.wannapik.com/   Even though ChIP requires ...Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein...protein:DNA interactions. ChIP works by using antibodies to capture protein:DNA complexes with antibodies...downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for exploring...an approach for the faint of heart!  The Process ChIP can be done for either tissues or cells. The process...step. For this blog, we're going to focus on the ChIP process and not the upsteam sample collection process...process or the downstream analysis process. And ChIP itself begins at...  Crosslinking Once you have your...

...Fc region may be changed.   Chimeric antibodies can help A chimeric antibody is made by changing an... are subtle, chimeric antibodies should be revalidated before use. In addition, chimeric antibodies will... a similar problem, then isotype converted, or chimeric, antibodies may be the right solution for you.... B antibody to a mouse IgG2a Fc. The resulting chimeric antibody would have the original variable regions...isotype (Liddell 2013). In most cases, the resulting chimeric antibody retains the binding specificity to the...utility of recombinant antibody tools, the resulting chimera may have different binding properties (Morelock...isotype-specific secondary antibodies when multiplexing with chimeric antibodies. In the case above you should use an...

...on to work in patent law, business development, teaching, etc. Church also encourages his mentees to follow...

...plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016). “...

...unrelated worm (C. elegans) [2]. Delivering dsRNA matching a specific mRNA sequence triggers a cascade of... through physical blocking of the translation machinery. In the Sánchez lab protocol, dsRNAs are delivered...

...knockout in immune systems.   CHIME We previously developed CHIME (CHimeric IMmune Editing system) to enable...have not been widely employed in vivo. The CHIME and new X-CHIME systems, developed and deposited at Addgene...hindering studies post embryogenesis. Using C-CHIME and S-CHIME, we discovered that double knockout of PTPN1...Schematic showing the X-CHIME approach. Figure from LaFleur, et al. 2024.   The X-CHIME deposit also includes...preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a...LaFleur et al., 2019a and LaFleur et al., 2019b). CHIME works through transduction of Cas9-expressing (Rosa26...quite useful for interrogating these scenarios. X-CHIME We recently developed and deposited four new systems...

... a scientist, it is useful to make literature searching and reading a regular part of your routine. Set...scientific literature a little bit at a time. Searching for literature My go-to search engines for finding...a network with an institutional subscription), archived on PubMed Central, or in preprint onBioRXIV. One...Mendeley – these programs offer more options for searching and sorting your own digital library as well as...some integration with internet-wide literature searching. Update 5/6/2015:Here's another option that may... of their F1000Workspace.  Give your favorite searching, reading and organization tips in the comments...

...and have coaching plans that are financially accessible. Sometimes only a few hours of coaching time can...Some have online coaching tools that can be very effective and also help keep the coaching costs low. Most...also launched an online career coaching program that might make her coaching more affordable for you. There...making this transition). Another coaching group is Still Point Coaching. One nice thing about a group is...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...exception, that this helps people do a better job at reaching their goals.  Check out Joanne's Reddit AMA I...that few scientists are aware of the benefits of coaching relationships and do not know where to look for...

...engineered DNA-binding molecule-mediated ChIP (enChIP). By using enChIP, specific genomic regions can be easily...technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP are recent examples. We also...locus-specific ChIP). These methods consist of insertional chromatin immunoprecipitation (iChIP) and engineered...the insertional chromatin immunoprecipitation (ChIP) (iChIP) technology, which uses locus-tagging and affinity...requested plasmids for enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene...molecule-mediated chromatin immunoprecipitation (enChIP), both developed in the lab. In June 2014, Dr. ...  Addgene: Your lab has worked extensively with enChIP systems. Can you describe this technology and its...

...the interview with Joachim Goedhart Podcast breakdown Intro Chapter 1 - How Joachim ended up at the University...art. Outro Many thanks to our guest, Joachim Goεdhart! Joachim Goedhart is an assistant professor at...Scientist Benoit Giquel. Ben recently spoke with Joachim Goεdhart, a professor at the University of Amsterdam...University of Amsterdam Chapter 2 - Joachim’s Academic and career goals. Career advice for people going... microscopy. Chpater 4 - Research projects in Joachim’s lab. New microscopy probes and more. Chapter 5...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   Special thanks to our sound engineer/producer...Fluorescent Proteins featured topic page Read Joachim's Series on Choosing the B(right)est Fluorescent...

... our paper, “Synthetic recombinase-based state machines in living cells” (1), into a more straight-forward...our engineered devices, recombinase-based state machines for your own experimental needs. For more context...the paper. Why might one be interested in state machine technology? Biological research has produced a ...timing of these factors. Recombinase-based state machines (RSMs), which take on a particular DNA-sequence... set of possible downstream states that can be achieved by expression of other recombinases. Multiple ...terminators, etc) are composed. Fear not – the state machine design space is highly degenerate and often times...Roquet et al., Synthetic recombinase-based state machines in living cells. Science. 353, aad8559 (2016)....

...naïve questions. During the panel, an artist was sketching her interpretation of the discussion in markers...

...gene. CHIME can also be used for high throughput screens using pooled libraries. Find the CHIME plasmids...successfully visualized in vivo using ultrasound in Escherichia coli and Salmonella Typhimurium. This work demonstrates...cell lineages in vivo using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen ...the Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any...

... (2002): 682-688. PubMed PMID:12404098.  4. Lin, Chi-Wang, Cindy Y. Jao, and Alice Y. Ting. "Genetically... which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...compacted it becomes. This prevents the cellular machinery from physically interacting with the genes in ...to have occurred that was passed down to the grandchildren; in this study,epigenetic changes were statistically...influenced cardiovascular mortality of the female grandchildren." BMC genetics15.1 (2014): 12. PubMed PMID: ...

...219444 Anti-KChIP1 [K55/9R] KChIP1 Rat Mouse IgG2a 219445 Anti-KChIP1 K+ channel [K55/59R] KChIP1 K+ channel...190284 Anti-KChIP3 K+ channel [K66/27] KChIP3 K+ channel Rat Mouse IgG2a 190285 Anti-KChIP3 K+ channel ... 206760 KChIP3 K+ channel scFv [K66/27] K66/27 scFv KChIP3 K+ channel Rat Mouse 206761 KChIP3 K+ channel...38 scFv KChIP3 K+ channel Rat Mouse 206762 KChIP3 K+ channel scFv [K66/59] K66/59 scFv KChIP3 K+ channel...Human Mouse IgG2a 177442 Anti-Pan-KChIP K+ channel [K55/82R] Pan-KChIP K+ channel Mouse IgG2a 177443 Anti-CASK...CASK Human Mouse IgG2a 177444 Anti-KChIP2b K+ channel [K60/73R] KChIP2b K+ channel Rat Mouse IgG2a 177445...external) Human Mouse IgG2a 188175 Anti-KChIP2b K+ channel [K60/87R] KChIP2b K+ channel Rat Mouse IgG2a 188176...

...Anhydrotetracycline (aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia coli Dominic...pBAD Arabinose Escherichia coli Christian Raetz 26098 pCW-LIC 3xPtac Lactose/IPTG Escherichia coli Cheryl...Lactose/IPTG Escherichia coli Cheryl Arrowsmith 43795 pDusk pR_FixK2 Blue light (470 nm) Escherichia coli Andreas... Lactose/IPTG Escherichia coli Cheryl Arrowsmith 46886 pMSP3535 PnisA Nisin Escherichia coli , gram-positive...ε-caprolactam Escherichia coli , Streptomyces sp. Xuming Mao 46888 pMSP3545 PnisA Nisin Escherichia coli , gram-positive...pPrpB Propionate Escherichia coli Jay Keasling 17810 pPro33 pPrpB Propionate Escherichia coli Jay Keasling...sensing molecule) Escherichia coli Cynthia Collins 84689 pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium...

...Elsaesser 140020 pAS_4xMma PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian...Bacterial Jason W Chin 174078 pDule-3-nitroTyrosine (A7) 3NY (A7) synthetase M. jannaschii 3-nitroTyrosine...Acd-A9 M. jannaschii acridone Bacterial TAG Ryan Mehl 197652 pDule2 - Mj Acd A9 Acd-A9 M. jannaschii acridone...key modifications to the standard translation machinery are required: The ncAA is generally supplied in...UGA). The amber codon is the rarest codon in Escherichia coli , which makes it the most commonly used ...independently of the host cell's natural genetic machinery. The orthogonal aaRS enzyme should not recognize.... tRNA and aaRS pairs from Methanocaldococcus janaschii are orthogonal in E. coli , while E. coli pairs...

...Purify Genomic Regions The enChIP (engineered DNA-binding molecule-mediated ChIP) system, developed by the...the DNA, to create a DSB enChIP En gineered DNA-binding molecule-mediated ChIP , using a tagged-dCas9+gRNA...the ways the CRISPR components are leveraged to achieve specific goals, such as targeting multiple genomic...HDR) pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing...gRNAs into a single plasmid — though some have achieved targeting in the double digits. These multiplex...nickase system is utilized in many CRISPR systems to achieve specific genetic edits. The dCas9 molecule binds...or G, Y = C or T SpRYc — SpRY PAMs plus NNG; a chimeric version of SpRY Though these PAM-flexible Cas9s...

... Special considerations One type of ChIP assay, called native ChIP, does not use cross-linking and instead...accessible to antibodies. Detection of sdAbs is usually achieved by conjugation of a signaling molecule directly...can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to distinguish...detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody is done through...Immunoprecipitation (IP) Chromatin immunoprecipitation (ChIP) Visualization - used to visualize protein location...either X-ray film in a darkroom or an image capture machine. Special considerations The primary antibody must...immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) allow you to isolate specific proteins or protein...

...Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NCerulean - Zebrafish/Xenopus/Worm/Sea urchin mCerulean N1 - Mammalian...Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NeGFP - Zebrafish/Xenopus/Worm/Sea urchin pKT0128 - Yeast Expression...Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus/Worm/Sea urchin pET mCitrine LIC cloning...Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NmOrange - Zebrafish/Xenopus/Worm/Sea urchin mOrange-N1 - Mammalian...Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NmCherry - Zebrafish/Xenopus/Worm/Sea urchin pET mCherry LIC cloning...

... Angew Chem Int Ed Engl. 2018 Jun 27. Tetsuya Kitaguchi ATP Variants of ATP sensor iATPSnFR1.0 and iATPSnFR1.1.... PLoS One. 2014 Jun 24;9(6):e100252. Tetsuya Kitaguchi cAMP (cyclic AMP) Red fluorescent protein-based...imaging. Sci Rep. 2017 Aug 4;7(1):7351. Tetsuya Kitaguchi cAMP (cyclic AMP) Fluorescent sensor of cyclic...PDE5alpha. ACS Sens. 2017 Jan 27;2(1):46-51. Tetsuya Kitaguchi cGMP (cyclic GMP) FlincG3 (GFP-based cGMP sensor...PLoS One. 2014 Jun 5;9(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic...Anal Chem. 2019 Apr 2;91(7):4821-4830. Tetsuya Kitaguchi Glucose Red fluorescent glucose indicators for...ratio changes in different cellular compartments. Biochim Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519...

...pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF, 108 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF,...fungi, protists, and viruses. Developed by the Joachim Wittbrodt and Juan L. Mateo (Link opens in a new...sequences. Organisms include human, mouse, rat, chicken, frog, zebrafish, fly, worm, Japanese rice fish...design and cloning; injection protocol pU6-BbsI-chiRNA ; phsp70-Cas9 PDF, 109 KB Orkin and Bauer Protocol...

...particular gRNA. In the enChIP (engineered DNA-binding molecule-mediated ChIP) system, catalytically inactive... used for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing ...researchers have expanded chromatin immunoprecipitation (ChIP) to allow purification of any genomic sequence specified...

...MDH-related research into their teaching laboratories. They encourage instructors teaching different types of courses...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and...development, membership to a local hub of educators teaching MDH CUREs, and opportunities to mentor future ...

... Description Category PI 157970 pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin...subretinal injection. It was identified by John Chiorini and characterized by Shannon Boye’s lab. AAV44.9...Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26(3):165-76. ...in future publications, please acknowledge John Chiorini and Shannon Boye and cite Boye, et al. 2020. Novel...

...EB1 EGFP Ken-Ichi Takemaru 122871 GFP-hCCDC11 Centriolar satellites CCDC11 EGFP Ken-Ichi Takemaru 54234...Mykytyn *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...Harrison *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...Fanning *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein... Pagano *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent Protein...

...nucleus accumbens medial shell and behavioral switching. Neurochemistry International 129:104482. PubMed...opens in a new window) Yu K, Ahrens S, Zhang X, Schiff H, Ramakrishnan C, Fenno L, Deisseroth K, Zhao ...in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons in ...in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1...hypothalamus-habenula circuit controls aversion. Mol. Psychiatry 24(9):1351-1368. PubMed (Link opens in a new ...

... and its associated proteins or RNAs through ChIP (enChIP) or biotin-streptavidin pulldown (CAPTURE.) ...molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun . 439...for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product....Myers RM. 2015. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res . 25(10)...