We narrowed to 931 results for: TIM

...Rossi Michael Ruppert Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka...

... Anhydrotetracycline (aTc) Staphylococcus aureus Tim Foster 36267 pBAD33.1 pBAD Arabinose Escherichia ...di-GMP Fluorescence (GFPmut2) Pseudomonas aeruginosa Tim Tolker-Nielsen 90217 pMV762-Peredox-mCherry NADH:...Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein Interactions Förster resonance...levels or to turn on expression only at a specific time. This collection contains plasmids whose expression...+ LacI Lactose/IPTG Corinebacterium glutamicum Timothy Lu 17972 pSE100 Pmyc1/TetO Anhydrotetracycline ...Prp PprpD2 Propionate Corinebacterium glutamicum Timothy Lu 36252 pRibo EcoHind Riboswitch Teophylline Escherichia...detect the presence of that small molecule and sometimes even quantify it. Bacterial one-hybrid (B1H) systems...

....(cyto).cpSFGFP.HaloTag CAG None Cre dependent 5 Tim Brown and HHMI-JRC Tool Translation Team 114471 pAAV-Ef1a-fDIO...

...S29W.A95K.HaloTag CAG iATPSnFR2 none Constitutive 5 Tim Brown , JRC Tools 5-HT Sensors 140552 pAAV-hsyn-GRAB...

...or extracellular glucose in vivo in mouse brain. Tim Brown , HHMI-JRC Tool Translation Team Glutamine ... that reports with lifetime and intensity changes A turquoise fluorescence lifetime-based biosensor for...Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. Nat Commun...Calcium GCaMP5 genetically encoded calcium sensor Optimization of a GCaMP calcium indicator for neural activity...variants (Constitutive or Cre-dependent) jYCaMP: an optimized calcium indicator for two-photon imaging at fiber... Indicator with an Expanded Dynamic Range by Optimization of Amino Acid Linkers between a Fluorescent ...Robust and Sensitive GFP-Based cGMP Sensor for Real Time Imaging in Intact Caenorhabditis elegans. Genetics...

...MGC20661 CSF1 colony stimulating factor 1 (macrophage) MCSF, MGC31930 CSF1R colony stimulating factor 1 receptor...CSF3 colony stimulating factor 3 (granulocyte) G-CSF, GCSF, MGC45931 CSF3R colony stimulating factor 3 receptor...LXA4R FSHB follicle stimulating hormone, beta polypeptide - FSHR follicle stimulating hormone receptor FSHRO...MGC141920 TSHB thyroid stimulating hormone, beta CHNG4, TSH-BETA TSHR thyroid stimulating hormone receptor ...physical barriers such as the skin and mucosa, antimicrobial peptides and proteins in the blood such as interferons...presenting its cognate antigen along with co-stimulation activates the T cell, whereas T cell tolerance...are induced when the TCR is engaged without co-stimulation or with a low affinity antigen. TGFbeta In the...

...conjugate or conjugation process. Sodium azide, an antimicrobial agent, can block some amines needed for conjugation...In those cases, thimerosal can be used as an antimicrobial agent. Other chemical interferences with conjugating...measurable light or color in response to a specific stimulus, can be conjugated to antibodies in order to allow...produces a colored product that accumulates over time which is relative to the amount of enzyme present...purchased already conjugated, which can save significant time in the lab. However, if the antibody you want is...ubiquitously expressed protein such as GAPDH or tubulin (sometimes referred to as housekeeping genes). This allows...protein or protein interactions in a specific timeframe or context. Immunoprecipitation (IP) IP uses immobilized...

...turnaround time. Any timing expectations communicated by email will supersede the general estimated turnaround...any adjustments to the original order. Turnaround Time Production of a Packaged on Request material is ...verification, may not yet have been completed at the time of shipment. If any discrepancies are identified... we do not expect to meet the general turnaround time, Addgene will notify you by email before, or within...turnaround time above. After starting viral production, if we determine that we will not meet the turnaround...turnaround time we described here or by email, Addgene will contact you to offer some options for moving forward...

...be low, but generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere... and base editor variants often have different optimal editing windows. Be sure to refer to the literature...gRNAs Once your target sequences are selected, it’s time to design your gRNA oligos and clone them into your...and express these components (Figure 2), and the optimal system will likely depend on your cell type. Figure...will work for your experiment) and the expression time (transient or stable). For plasmid and viral-based...best for your experiment. It may be necessary to optimize your delivery conditions, especially if you are... gRNA and Cas enzyme to your target cells, it is time to validate your genome edit. CRISPR editing produces...

...contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA. The...contains a single cassette expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons...Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ordered via the links below... S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single...contain two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus...contains a single cassette expressing human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as ...contains a single cassette expressing human codon-optimized SpCas9, driven by the truncated MeCP2 promoter...

... an optimized gRNA for your application (see: gRNA Design Software ). In addition to an optimized gRNA... currently no defined measure of fidelity, so sometimes they will be referred to as increased fidelity...structured motif to enhance pegRNA stability PEmax — optimized PE architecture; increased synergy between PE ...compared to HDR. Prime editing requires more optimization and typically has lower efficiency (< 50%) than...integrase landing site ( attB ) and the other to stimulate DNA repair. This can result in more efficient .... gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity...activators and targeting promoter regions. You might sometimes see these referred to together as CRISPRi/a. dCas9...

...integration efficiency but research groups continue to optimize these tools. The best characterized SSR systems...Expression By expressing the enzyme at specific times or locations using cell-specific promoters or inducible...inducible systems, you can precisely control and optimize expression of your gene of interest. Inducible...recombination of the DNA between target sites. Codon optimization: Modifying the DNA sequence of the recombinase...permit gene expression until Cre is present, at which time the gene will be disrupted or deleted. shRNA Expression... Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101...

...application of innovative tools enabling large-scale real-time interrogation and monitoring of brain cells and ...198513-AAV1 pAAV_hSyn-PdCO-EGFP-WPRE Expresses optimized PdCO in frame with EGFP under control of human...198513-AAV5 pAAV_hSyn-PdCO-EGFP-WPRE Expresses optimized PdCO in frame with EGFP under control of human..._EF1a-DIO-PdCO-mScarlet-ER-miniWPRE Expresses optimized PdCO in frame with mScarlet under control of eukaryotic...soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor...

...acceptor. Alternatively, the fluorescence lifetime (the time between excitation of the fluorophore and...of the donor can be measured using fluorescence lifetime imaging microscopy (FLIM) or FLIM-FRET, where ...energy transfer through FRET reduces the donor lifetime. FRET is often used to study (1) protein-protein...fluorescent protein expression level and maturation time, and other environmental factors. When designing...

...luciferase (Gluc) NanoLuc® (Nluc) The mammalian codon-optimized version of firefly luciferase is often referred...luciferase is secreted and more stable, enabling time course experiments, but is too dim for some applications...modified) CMV Optical reporter of presynaptic ATP Timothy Ryan 24348 pAC-hRluc Renilla Actin5C Insect expression...luciferase and a Halo tag to assay mRNA turnover in real time. Nonsense-mediated decay reporter from Baird et ...® protein production. Other Sensors and Tools Optimized STARR-seq (Self-Transcribing Active Regulatory...

...Save time by ordering cloning grade DNA for over 200 of our most popular plasmids!...arrival of their plasmid from Addgene – reducing the time to experiments by removing the amplification and...order more than 2 µg of cloning grade DNA? At this time, to order more than 2 µg of a single plasmid, multiple...addgene.org . Don’t See What You’re Looking For? At this time, Addgene has made a limited number of plasmids available...

... This comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids...fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based ...Plasmids for constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid...new window) Sheff, M. A., Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces...

...repurposing endogenous systems that respond to natural stimuli like hormones or metal ions, scientists have adapted...promoter, and other tet- or dox-dependent promoters (sometimes generally called pTet) have been designed by combining...Resnitzky et al., 1994). TRE3G promoter : also optimized into P TRE3GV for viral expression and P TRE3GS...in mammalian cells or other species by codon optimization of their sequence and eliminating interactions...Klaver, B., Berkhout, B., & Das, A. T. (2006). Optimization of the Tet-On system for regulated gene expression...

...high sensitivity. These assays are ideal for real-time monitoring of signaling pathways, investigating ... your specific purpose. HaloTag technology is optimized for various protein expression systems including...substrate. NanoBiT PPI assays enable kinetic, real-time analysis of protein interaction, association, and...you to detect PPI induction and inhibition in real time using full-length proteins or fragments. NanoBRET...

...serves the needs of many researchers, scientists sometimes require preps beyond our current in-stock capabilities...approval of the MTA, and shipping, mean that the actual time from request to delivery could be up to 10 weeks...Production 6–8 weeks Quality is our priority. During this time, we amplify DNA, produce the prep, and perform comprehensive...information as soon as your order ships. Shipping time will depend on your location. Share Your Results...

...embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding, and transparency...proteins to visualize and track clones of cells over time. Examples of useful tools for studying cells that...clonal origin in zebrafish can be found below: Optimized QF-binary expression system for use in zebrafish...vertebrate model to study ALS-linked aggregates in real time. Live imaging of astroctyes - Kelly Monk Lab. Live...

...Explore plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene ...Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants. CRISPR Sylvestre...expression cassettes. A CRISPR/Cas system designed and optimized for work in the model plant Nicotiana benthamiana...Siliques Tips for Arabidopsis Transformation Save Time with Transient Plant Leaf Transformations Engineering...

...characterized by the progressive degeneration and ultimate death of neurons. Common neurodegenerative diseases...Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive...motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads to limb weakness,...CRISPRoff-v2.1 . (Nuñez et al. Cell. 2021) Conduct multimodal genetic screens in human iPSC-derived neurons...

... uses an open-source algorithm to automate and optimize gRNA and HDR donor design. The current version...Link opens in a new window) Program for designing optimal gRNAs. Provides feedback on number of potential...human. Developed by the George Church Lab . CRISPR Optimal Target Finder (Link opens in a new window) Identification...vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning...

...mutant to inhibit MMR PEmax — optimized PE2 enzyme containing RT optimized for human codons, additional...PE2–PE5 systems PE6 — small prime editors with optimized RT and/or Cas9 domains PE7 — addition of RNA-binding...

...Photoactivatable Photoconvertible Photoswitchable Timers Self-Labeling Tags Additional Resources The following...drops to half its maximum value. Maturation is the time for fluorescence to reach half its maximal value... Bacterial Expression Return to top Fluorescent Timers Fluorescent protein variants that change their ...their fluorescence from the blue to red over time, allowing tracking of processes such as intracellular trafficking...

...Link opens in a new window) designed and codon-optimized the Open Enzymes parts for expression in Escherichia...pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB...pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI...

...generated using standard methods that have been optimized for each specific virus in order to generate high...Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

...production, secretion, and codon-optimized versions designed for your expression system. ...SARS-CoV materials are available only in the US at this time. Return to top Plasmids Encoding Mammalian Genes...Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain and...

... TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector...Sangamo)-Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled...replacing pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...