...Rossi Michael Ruppert Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka...

... that reports with lifetime and intensity changes A turquoise fluorescence lifetime-based biosensor for...or Cre-dependent) Cell-specific restoration of stimulus preference after monocular deprivation in the ...Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology. Nat Commun...Calcium GCaMP5 genetically encoded calcium sensor Optimization of a GCaMP calcium indicator for neural activity...variants (Constitutive or Cre-dependent) jYCaMP: an optimized calcium indicator for two-photon imaging at fiber... Indicator with an Expanded Dynamic Range by Optimization of Amino Acid Linkers between a Fluorescent ...Robust and Sensitive GFP-Based cGMP Sensor for Real Time Imaging in Intact Caenorhabditis elegans. Genetics...

...MGC20661 CSF1 colony stimulating factor 1 (macrophage) MCSF, MGC31930 CSF1R colony stimulating factor 1 receptor...CSF3 colony stimulating factor 3 (granulocyte) G-CSF, GCSF, MGC45931 CSF3R colony stimulating factor 3 receptor...LXA4R FSHB follicle stimulating hormone, beta polypeptide - FSHR follicle stimulating hormone receptor FSHRO...MGC141920 TSHB thyroid stimulating hormone, beta CHNG4, TSH-BETA TSHR thyroid stimulating hormone receptor ...physical barriers such as the skin and mucosa, antimicrobial peptides and proteins in the blood such as interferons...presenting its cognate antigen along with co-stimulation activates the T cell, whereas T cell tolerance...are induced when the TCR is engaged without co-stimulation or with a low affinity antigen. TGFbeta In the...

...conjugate or conjugation process. Sodium azide, an antimicrobial agent, can block some amines needed for conjugation...In those cases, thimerosal can be used as an antimicrobial agent. Other chemical interferences with conjugating...measurable light or color in response to a specific stimulus, can be conjugated to antibodies in order to allow...produces a colored product that accumulates over time which is relative to the amount of enzyme present...purchased already conjugated, which can save significant time in the lab. However, if the antibody you want is...ubiquitously expressed protein such as GAPDH or tubulin (sometimes referred to as housekeeping genes). This allows...protein or protein interactions in a specific timeframe or context. Immunoprecipitation (IP) IP uses immobilized...

...Vary these times depending on the doubling time of the cell line used. NOTE: This incubation time allows ... such as cationic liposome-based transfection. Optimize transfection conditions for each cell line with...culture media after electroporation. Minimize the time between electroporation and transferring the solution...dilution at 30 cells per 96-well round-bottom plate. Optimize plating the cell type used at limiting dilution...1 min, 72 °C for 1 min), and 72 °C for 10 min. Optimize PCR conditions for each primer pair designed based...non-deletion” PCR primer pairs in a single reaction. Optimize multiplexing in a polyclonal population ( i.e.... the same PCR primers and reaction conditions optimized on the bulk cells (see step 6 ). Select the clones...

...turnaround time. Any timing expectations communicated by email will supersede the general estimated turnaround...any adjustments to the original order. Turnaround Time Production of a Packaged on Request material is ...verification, may not yet have been completed at the time of shipment. If any discrepancies are identified... we do not expect to meet the general turnaround time, Addgene will notify you by email before, or within...turnaround time above. After starting viral production, if we determine that we will not meet the turnaround...turnaround time we described here or by email, Addgene will contact you to offer some options for moving forward...

...that’s interesting from an applied perspective, sometimes it’s best to delete genes from or introduce new...N-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. pET-Sangamo-His 40787...C-terminally His-tagged TALEN fused to an E. coli codon optimized FokI endonuclease domain. Return to Top Visualization...His-tagged cyan fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent...His-tagged yellow fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent ...this plasmid. Return to Top Protein Purification Sometimes looking at a protein inside cells just isn’t enough...levels or to turn on expression only at a specific time. This collection has a variety of plasmids whose...

...contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA. The...contains a single cassette expressing human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons...Nature Protocols 2013). Individual human codon-optimized SpCas9 plasmids can be ordered via the links below... S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single...contain two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus...contains a single cassette expressing human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as ...contains a single cassette expressing human codon-optimized SpCas9, driven by the truncated MeCP2 promoter...

... an optimized gRNA for your application (see: Plan Your Experiment ). In addition to an optimized gRNA... currently no defined measure of fidelity, so sometimes they will be referred to as increased fidelity...structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between PE ...compared to HDR. Prime editing requires more optimization and typically has lower efficiency (< 50%) than...integrase landing site ( attB ) and the other to stimulate DNA repair. This can result in more efficient .... gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity...activators and targeting promoter regions. You might sometimes see these referred to together as CRISPRi/a. dCas9...

...enables visualization of Cre expression. Optimized Cre: Codon-optimized Cre ( iCre ) is expressed at higher...pBF3038 Cre codon optimized for yeast Gal1 Yeast Sandmeyer 26853 pBF3060 Cre codon optimized for yeast Gal1...when Cre is present. By expressing Cre at specific times or locations, you can precisely control expression... ways to tightly control Cre expression and to optimize Cre expression once it is induced. Addgene has...insulin Zebrafish Stainier 24593 AAV-pgk-Cre codon optimized Cre PGK AAV Aebischer 24704 GFAP-Cre Cre GFAP ...permit gene expression until Cre is present, at which time the gene will be disrupted or deleted. Cre-dependent...

...application of innovative tools enabling large-scale real-time interrogation and monitoring of brain cells and ...198513-AAV1 pAAV_hSyn-PdCO-EGFP-WPRE Expresses optimized PdCO in frame with EGFP under control of human...198513-AAV5 pAAV_hSyn-PdCO-EGFP-WPRE Expresses optimized PdCO in frame with EGFP under control of human..._EF1a-DIO-PdCO-mScarlet-ER-miniWPRE Expresses optimized PdCO in frame with mScarlet under control of eukaryotic...soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor...

...version of Firefly luciferase that has been codon optimized for expression in mammalian systems. Other luciferases...Click-beetle red luciferase has also been codon optimized ( CBR2opt ) for mammalian expression systems. ...Gaussia luciferase is secreted and more stable, making time course experiments possible, but its short luminescence...luciferase and a Halo tag to assay mRNA decay in real time. pKC-4.04, pKC-4.06 : Nonsense-mediated decay reporter...luciferase. Transcriptional Element Screening Optimized STARR-seq ( S elf- T ranscribing A ctive R egulatory...modified) CMV Optical reporter of presynaptic ATP Timothy Ryan 24348 pAC-hRluc Renilla Actin5C Insect expression...

...experiments, consider that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been...Zhang lab, which has comparable efficiency to the optimized chimeric design but with better accuracy (in other...similar to using a single chimeric gRNA. At the same time, the off-target effects are reduced because the ...efficient guide (most guides would work, but they sometimes have different efficiency). And it is best if ...the publication of our paper, we have two new optimized primers that may work better than the published...

...putative target sites have been identified, it is time to choose which site is likely to result in the ...Using validated gRNAs can save your lab valuable time and resources when carrying out CRISPR experiments...Once you have selected your target sequences it is time to design your gRNA oligos and clone these oligos...proceeding with your experiment, it may be necessary to optimize your delivery conditions. Learn more about CRISPR... gRNA and Cas enzyme to your target cells, it is time to validate your genome edit. CRISPR editing produces...

... This comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids...fusions of proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based ...Plasmids for constitutive overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid...Link opens in a new window) Sheff MA, Thorn KS. Optimized cassettes for fluorescent protein tagging in Saccharomyces...

...Save time by ordering cloning grade DNA for over 200 of our most popular plasmids!...arrival of their plasmid from Addgene - reducing the time to experiments by removing the amplification and...order more than 2 µg of cloning grade DNA? At this time, to order more than 2 µg of a single plasmid, multiple...addgene.org . Don’t See What You’re Looking For? At this time, Addgene has made a limited number of plasmids available...

...Inhibition Human Adamson 3rd ∼3 366 or 1,573 Activity-optimized genome-wide library Discontinued Knockout Human...Human Sabatini and Lander 3rd 10 178,896 Activity-optimized genome-wide library 1000000100 Knockout Human ...Knockout Human Yusa 3rd 5 90,709 Human Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout ...Turner 3rd 10 8,260 Two plasmid human activity-optimized genome-wide library 1000000095 Knockout Human ...Lander 3rd 10 187,536 Two plasmid mouse activity-optimized genome-wide library 1000000096 Knockout Mouse ...

...plasmid ( pdCas9_humanized ) contains a human codon optimized dcas9 gene under the control of Murine Stem Cell...humanized A catalytically inactive, human codon-optimized Cas9 expression plasmid 44247 pdCas9::BFP-humanized...BFP-humanized A catalytically inactive, human codon-optimized Cas9-BFP fusion expression plasmid 44248 pgRNA-humanized...expression plasmid for use with either human codon-optimized Cas9 constructs 44249 pdCas9-bacteria A catalytically...

...serves the needs of many researchers, scientists sometimes require preps beyond our current in-stock capabilities...approval of the MTA, and shipping, mean that the actual time from request to delivery could be up to 10 weeks...Production 6–8 weeks Quality is our priority. During this time, we amplify DNA, produce the prep, and perform comprehensive...information as soon as your order ships. Shipping time will depend on your location. Share Your Results...

...embryogenesis is complete in ~3 days), short generation time (~3 months), ease of care and breeding, and transparency...proteins to visualize and track clones of cells over time. Examples of useful tools for studying cells that...clonal origin in zebrafish can be found below: Optimized QF-binary expression system for use in zebrafish...vertebrate model to study ALS-linked aggregates in real time. Live imaging of astroctyes - Kelly Monk Lab. Live...

...website contains additional information CRISPR Optimal Target Finder Identifies gRNA target sequences ...below: ID Plasmid 46294 pBS-Hsp70-Cas9 : A codon-optimized Cas9 nuclease under the control of the Drosophila...hsp70 promoter. 45945 pHsp70-Cas9 : The codon-optimized Cas9 nuclease under the control of the Drosophila...

...intensity is 50% of its maximum value. Maturation is the time for fluorescence to obtain half-maximal value. Structure...Photoactivatable Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation (nm) Emission (nm) Brightness... - Mammalian Expression Jump to Top Fluorescent Timers Protein Excitation (nm) Emission (nm) Brightness...with a wide variety of colors, some specifically optimized for yeast Lindquist Lab Vectors - Gateway cloning...

...mutant to inhibit MMR PEmax - optimized PE2 enzyme containing RT optimized for human codons, additional...PE2–PE5 systems PE6 - small prime editors with optimized RT and/or Cas9 domains PE7 - addition of RNA-binding...

...tag pAquaN1 Cyan Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express...pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet...pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial Expresses YPet...

...Link opens in a new window) designed and codon-optimized the Open Enzymes parts for expression in Escherichia...pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB...pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI...

...generated using standard methods that have been optimized for each specific virus in order to generate high...Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

...of recoded E.coli (rEcoli XpS 192872 ) and an optimized phosphoserine orthogonal translation system (pSerOTS-C1... recoded strain of E. coli ( 68306 ) with the optimized phosphoserine orthogonal translation system ( ... and a new pSerOTS described in: System-wide optimization of an orthogonal translation system with enhanced...

... TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector...Sangamo)-Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled...replacing pTAL4. The pTAL7 destination plasmids were optimized for TALEN expression in mammalian systems including...

...promoters had been identified that responded to stimuli, such as hormones or metal ions; however, these...pursue a non-endogenous system for eukaryotes. At the time, bacterial systems from E. coli presented the best...Biotechniques . 2004 Oct;37(4):546, 548, 550. PubMed . Optimization of the Tet-On system for regulated gene expression...

...characterized by the progressive degeneration and ultimate death of neurons. Common neurodegenerative diseases...Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive...motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads to limb weakness,...