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We narrowed to 154 results for: 11

Showing: 1 - 40 of 154 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...CRISPR effector protein they named Cas7-11. Testing showed that Cas7-11 cut RNA with unprecedented specificity...luciferase RNA knockdown assays using Cas7-11   Use The Cas7-11 targeting system contains two pieces: ...both of which we're featuring in today's blog. Cas7-11 Development and Discovery As part of their quest ...looked at the transformed E. coli expressing Cas7-11, they found no evidence of growth inhibition when...negative effects in RNA targeting experiments. If Cas7-11 lacked this toxicity, it would represent a major ...  The team originally suspected that this new Cas7-11 might be phylogenetically classified as the more ...observations, Koonin and Makarova found that Cas7-11 appeared to have derived from the more complex type...

  2. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...are split between strand 10 and 11 to yield large (1-10) and small (11) fragments. In several cases an... FP(11) fragments, even from a different base FP (for example, EBFP2(1-10) can be used with GFP(11)). ... Figure 1: Two proteins are fused to the FP(11) and FP(1-10) fragments. When Protein A and B interact...EBFP2 EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split...Kamiyama Cerulean Cerulean(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split...-folder GFP, our most-requested GFP(1-10) and GFP(11) Versatile protein tagging in cells with split fluorescent...Brian McNaughton mNeonGreen2 mNG2(1-10) and mNG2(11) Improved split fluorescent proteins for endogenous...

  3. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...doing rotations? 8:01 - 11:25 : How did you decide what lab to join? 11:25 - 11:50 : What is the number...rotation students should ask senior graduate students? 11:51 - 13:28 : How do you define what a good mentor...What's been your biggest experimental failure? 26:11 - 31: 32 : What's been your biggest experimental ...

  4. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...FlipGFP, sfCherry was split into  β1-9 and β10-11, where β10-11 was forced into a parallel conformation by...different self-assembling split GFP (GFP1-9 and GFP10-11) by flipping a single β-strand of GFP, hence the ...et al., 2019). How does FlipGFP work? GFP contains 11 β-strands and one central ɑ-helix that can be split... ɑ-helix, (2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains components and residues...was dependent on protease activation. Normally, β10-11 are antiparallel. To prevent binding, the β strands...thus can no longer fit with β1-9. They connected β10-11 with two linkers (E5 and K5) that dimerize together...

  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Best for: targeting single stranded RNA.   Cas7-11 Cas7-11 is a naturally occurring RNase composed of the...between Cas7-11 and Cas13 are 1). non-specific RNA cleavage and 2). cell toxicity. Cas7-11 does not display...the fusion of several Cas7 and Cas11 domains. Cas7-11 cuts ssRNA and is capable of pre-crRNA self-processing... of collateral cleavage). You can read about Cas7-11 in our blog post on its development and use. Fast...targeting with the single-protein CRISPR effector Cas7-11. Nature, 597, 720-725 (2021). 10.1038/s41586-021-...

  6. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...enhanced two-photon absorption. Neurophotonics. 2024 Apr;11(2):024207. doi: 10.1117/1.NPh.11.2.024207. Robert... calcium indicators. J Biol Chem. 2019 Mar 15;294(11):3934-3946. Katalin Torok Calcium Red Calcium indicators... and multiplexing capabilities. bioRxiv. 2025 Jan 11:2024.12.16.628673. doi: 10.1101/2024.12.16.628673... in multiple cellular locations. PLoS One. 2012;7(11):e49371. Direct comparison of a genetically encoded...of cytosolic Zn(2+). ACS Chem Biol. 2013 Nov 15;8(11):2366-71. Amy Palmer Zinc Cell surface localized ...ratiometric fluorescent sensor. PLoS One. 2017 Nov 9;12(11):e0187481. Mathew Tantama ATP QUEEN ATP sensors for... cyclic AMP. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. Justin Blau cAMP (cyclic AMP) cAMPFIRE...

  8. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...give their insights as to what causes this dip. 11:11 - 15:37 : How have you changed throughout graduate....com/watch?v=lQGAsgjjBDI&feature=youtu.be. 8:17 - 11:10 : The 3rd year of graduate school and what makes...

  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ...interleukin 11 AGIF, IL-11 IL11RA interleukin 11 receptor, alpha MGC2146 IL11RB interleukin 11 receptor,... variable 11-55 (non-functional) IGLV1155, V4-6 IGLV2-11 immunoglobulin lambda variable 2-11 IGLV211, ...diversity 3-9 DXP1, IGHD39 IGHD4-11 immunoglobulin heavy diversity 4-11 (non-functional) DA1, IGHD411 IGHD4...variable 2-70 IGHV270, VH IGHV3-11 immunoglobulin heavy variable 3-11 (gene/pseudogene) IGHV311, VH IGHV3...variable 2D-40 IGKV2D40, O1 IGKV3-11 immunoglobulin kappa variable 3-11 IGKV311, L6 IGKV3-15 immunoglobulin...non-functional) IGKV37, L10, L10a, Vh IGKV3D-11 immunoglobulin kappa variable 3D-11 IGKV3D11, L20 IGKV3D-15 immunoglobulin...

  10. 8 Great Depositor Research Stories

    Type
    Blog Post
    ..., a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh and Jonathan Gootenberg...worked with us to share their new CRISPR tools, Cas7-11, a low-toxicity RNA editing tool, and PASTE, a drag-and-drop...

  11. CRISPR Antimicrobials

    Type
    Blog Post
    ... the proportion of virulent S. aureus from 50% to 11% in just 24 hours.   Figure 1: Single-phage...efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed: PMID 252409288. PubMed ...sequence-specific antimicrobials.” Nature Biotechnol. 32(11): 1146-50. (2014). PubMed: PMID 25282355. PubMed ... next-generation antimicrobials.” Genome Biol. 15(11):516. (2014). PubMed: PMID 25417800. PubMed Central...

  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    .... J Gen Virol., 68 (Pt 11), 2769–2780. https://doi.org/10.1099/0022-1317-68-11-2769. PMID 2445904.   ...new variation on their previous sensor STReTCh, the 11-amino acid BiT fragment of luciferase is embedded... Society. Both PILATeS (18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors...

  15. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ... iPS cells without antibiotic selection. Nat Meth 11, 291–293 (2014). PubMed PMID: 24509632. PubMed Central...Specific Screening of Genome Edited Cells. PLoS ONE 11, e0153901–17 (2016). PubMed PMID: 27089539. PubMed...-dPCR) mediated by designer nucleases. Nat Protoc 11, 598–615 (2016). PubMed PMID: 26914317. 4. Mock, ...

  17. Ras Pathway

    Type
    Collection
    ...Serine/threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2 Transcription...therapy. Downward J. Nat Rev Cancer. 2003 Jan;3(1):11-22. PubMed PMID: 12509763 . Do you have suggestions...

  18. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...substrates containing gaps or DNA base pair mismatches (8-11). For some applications, there cannot be any end-... near the ligation junction with high efficiency (11, 21). Ligases are thought to interrogate dsDNA for...thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. Lauer, G., et al. "Cloning...PubMed PMID: 3684572. PubMed Central PMCID: PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of...

  20. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...Additional human IgG1s are also available on demand. The 11 IPI synthetic rAbs, developed using yeast display... integrins, the RGD-binding integrins. Six of the 11 IPI antibodies contain RGD-mimetic sequences that...binding specificity, kinetics, and affinity of these 11 newly discovered synthetic rAbs. We then used these...

  21. Hot Plasmids - January 2023

    Type
    Blog Post
    ... mRNA with 90-99% knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm...via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021-00779-w. Epub...

  23. Typing CRISPR Systems

    Type
    Blog Post
    ...effector was developed, called Cas7-11. Despite being in Class 1, Cas7-11 is a single protein effector and...CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/10.1038/nrmicro3569 Makarova...targeting with the single-protein CRISPR effector Cas7-11. Nature, 597(7878), 720–725. https://doi.org/10.1038...

  25. Plasmids for Stem Cell Research

    Type
    Collection
    ...somatic cells to pluripotency. Cell Stem Cell. 2008 Sep 11. 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic...in human stem cells. Mol Syst Biol. 2014 Nov 17;10(11):760. Weiss iPSCs & iPSC-NPCs Neurons Lentiviral ...into GABAergic Interneurons. Cells. 2020 Nov 10;9(11). pii: cells9112451. doi: 10.3390/cells9112451. Ottosson...

  26. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...the OpenFlexure microscope," Biomed. Opt. Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy...open standard for microscopic imaging. Nat Commun 11, 5979 (2020). https://doi.org/10.1038/s41467-020-...

  27. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...updated CRISPR 101 e-book (to help with the other 11 presents!)  Can't get enough of CRISPR? Neither can...

  28. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...plants enabled by a biorecognition motif. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15731-w Wang... ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027 . https://doi.org/10.1371/journal.pone.0154027...

  29. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...with gene editing. CRISPRainbow requires very short, 11-mer gRNAs, which do not induce genome editing. If...editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed Central...

  30. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...site: TNA(C/G)TTATAA(G/C)TNA. Tol2 can deliver 10-11 kB to mammalian cells without decreased efficiency...payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, the gene mutated in muscular...Transposons: Nature and Applications in Genomics. CG 11:115–128. https://doi.org/10.2174/138920210790886871...

  32. CRISPR Guide

    Type
    Collection
    ...orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and Nme2Cas9 (from Neisseria meningitidis ...orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview of visualizing genomic loci with fluorophores...optogenetic control of CRISPR–Cas9. Nature Methods , 15 (11), 924–927. PMID: 30377362 Casini, A., Olivieri, M...CRISPR–Cas systems. Nature Reviews Microbiology , 13 (11), 722–736. PMID: 26411297 Marino, N. D., Pinilla-...using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A., Cong, L.,...double-stranded breaks. Biochemical Journal . 475 (11), 1955–1964. PMID: 29891532 Gaudelli, N. M., Komor...genes and phage host factors. PLOS Genetics . 14 (11), e1007749. PMID: 30403660 Sanson, K. R., Hanna, ...

  33. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...coefficient of a protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. PMID: 8563639...particle formation. J Pharm Sci. 2015 Feb;104(2):602-11. doi: 10.1002/jps.24259. Epub 2014 Nov 21. PMID: ...

  36. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Ten-fold Robust Expansion Microscopy (TREx). Elife 11:e73775. https://doi.org/10.7554/elife.73775  Dani...proteins in their ultrastructural context. Nat Commun 11:3850. https://doi.org/10.1038/s41467-020-17523-8 ...

  37. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. PubMed PMID: 9765382. PubMed Central PMCID...Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed Central PMCID...

  39. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...produce Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate...M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim...
Showing: 1 - 40 of 154 results