Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 402 results for: CAPS;

Showing: 1 - 40 of 402 results

  1. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10 ... B10 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22 ...and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S PHP.S...and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1 PHP.V1... and B22 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1... MaCPNS1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185137 pUCmini-iCAP-AAV.MaCPNS2...plasmid (non-standard), expressing Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Viviana...

  2. Caltech Systemic Capsids

    Type
    Collection
    ....V1 MaCPNS1 MaCPNS2 CAP-B10 CAP-B22 AAV9-X1.1 As part of our Viral Vector Packaging Service , Addgene ...Pubmed 35643078 (Link opens in a new window) CAP-B10, CAP-B22: Goertsen, Flytzanis, et al., 2022 Pubmed...Control Boyden CAP-B10 These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid...Control Boyden CAP-B22 These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B22 plasmid...plasmid (Addgene #175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP...plasmid (Addgene #175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP...AAV Caltech Systemic Capsids Viral Vector Packaging Service: Caltech Systemic Capsids PHP.eB PHP.S PHP.V1...

  3. Brain Armamentarium

    Type
    Collection
    ...Brain Science , Tanya Daigle James M. Wilson 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG...CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG...CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the...CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the... Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 230681-AAV5 AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA...

  4. Control AAV Preps

    Type
    Collection
    ...AAVrh10 AAV11 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10 CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter CAG CaMKIIa...Constitutive 1, 2, 5, 6, 8, 9, 11, rg*, PHPeB, CAP-B10, CAP-B22, MaCPNS1, MaCPNS2, AAV9-X1.1 Boyden 44332...

  5. AAV Viral Preps

    Type
    Collection
    ...neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1, MaCPNS2, CAP-B10, CAP-B22 University of Florida...

  6. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...plasmid aka the RepCap plasmid encodes two genes: the replication (rep) gene and the capsid (cap) gene. In ...into a capsid other than AAV2. This production strategy is called cross-packaging.  Capsid (cap) gene ...take a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products. Created...gene The cap gene encodes three AAV viral capsid proteins VP1, VP2, and VP3, which together form the outer...outer capsid shell that protects the viral genome. The cap gene is often driven by the AAV p40 promoter....variable loops is one method for changing a capsid’s tropism.  cap also encodes the Assembly-Activating Protein...recombinant AAV (rAAV) is made in the lab, rep and cap are expressed from a separate plasmid in trans, freeing...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...systemic capsids. After some consideration, we offer this definition: A systemic capsid is a capsid that ... Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a systemic capsid, developed ...may want to consider a systemic capsid for your AAV.   Systemic capsids While there is quite a bit of information...Image and caption courtesy of Challis et al., 2022. Where do they come from? Systemic capsids are typically...new systemic capsids for both rodents and non-human primates (NHPs). These engineered capsids have increased... the systemic capsid in question (or both). Therefore, a high-efficiency systemic capsid has better efficacy...precursor. Considering systemic capsids for your research? While systemic capsids mostly function like your ...

  9. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...earned a blue flame for its adenine base editing capabilities. Epigenetics: CRISPR has made it possible to...resources. Not on social media? No problem - see our recap below! Day 1: gRNA design tools: How to Design your...

  10. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ... Figure 2: Comparing CAPTURE and CAPTURE 2.0.     Original CAPTURE CAPTURE 2.0 Biotinylation ...between original CAPTURE and CAPTURE 2.0 methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased.... Find plasmids for CAPTURE 2.0! When compared to the original CAPTURE, CAPTURE 2.0 had a ~14-fold increase...Recently the Xu lab developed CAPTURE 2.0, an updated version of CAPTURE that detects chromatin interactions...Regulatory Elements: The original CAPTURE method The original CAPTURE method requires the creation of a.... From here, the CAPTURE method is similar to other 3C methods: crosslinking captures chromatin interactions...the use of CAPTURE with primary cells or rare cell populations. Key components of the CAPTURE 2.0 system...

  11. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...impact on this challenge either for magnetic capture or for capture using microfluidic devices. Addgene: What...S” in Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together. SpyLigase...antibodies against common tumor markers to subsequently capture cancerous cells expressing low levels of tumor ...binding stability really mattered for efficient capture. We wanted to be able to build a highly multivalent...for keeping going while we worked out the cell capture system.) Cancer and other applications Addgene:...technology mean in the context of cancer? Howarth: The capture of circulating tumor cells (CTCs) is going to have...treatment of cancer. It is clear that one needs to capture a diverse range of CTCs to cope with the variation...

  12. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...injecting CAP-B10 or CAP-B22 in marmosets, from an individual to another individual perspective? A: CAP-B10 ...CAP-B10 and CAP-B22 were tested individually in 4 marmosets each. Brain potency for each individual is reported...would you recommend using the PHP.S capsid over the PHP.eB (the capsid we have been using)?Would you recommend... insert with the CMV promoter using the AAV-BR1 capsid? A: It is our understanding that AAV-BR1 has the...recommend any liver specific capsid, particularly one that can target the epithelial cells in the blood-brain... AAV9 or AAV8. Very recently an engineered AAV5 capsid was reported for specific and potent liver transduction...recommend you keep an eye out for emerging new capsids in this area.   Q8: On dense labeling in the brain...

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...mutant AAV capsid (cap) expressing plasmids is generated. Diversity is introduced into the cap plasmid library...combinations into a region of the cap gene. In this special CREATE cap plasmid, loxP sites flank the downstream...especially for the study of pain. While many new capsids (i.e. the part of the virus that determines tropism...CREATE method to engineer two new IV injectable AAV capsids, AAV.PHP.eB and AAV.PHP.S, that target the CNS ...  Read on to learn more about these two new AAV capsids and how they can be useful tools for studying the...pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell type of interest...rounds of infection and screening by PCR enrich for capsid variants with increased ability to infect the cell...

  14. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ... follows: Cap Subject Industry H1B Academic H1B National Interest Waiver EB-1A EB-1B Cap subject industry...These numerically limited visas are referred to as “cap” subject H-1Bs. The H-1B allows U.S. employers to...application on their behalf.  If an individual obtains a cap subject H-1B (last year less than 30% of applicants...Four types of employers are not subject to the H-1B cap: institutions of higher education nonprofit entities...employers. H-1B petitioners are exempt from the cap if the applicant works at institutions of higher ...boutique consulting, investment banking and venture capital areas.  We place individuals into roles in areas...

  15. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...integration at the AAVS1 locus in humans. Cap is a structural capsid protein that helps with virion assembly...The viral packaging proteins for AAV are rep and cap. Rep is required for viral replication and also aids...from late endosomes when AAV is trafficked. Rep and cap mediate AAV pseudotyping which allows for altered...Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed for viral packaging...other viruses discussed. LXs/Iva2 are primarily capsid assembly proteins required for packaging of the...

  16. Lab Automation at Addgene

    Type
    Blog Post
    ...and help keep things more organized. Cap tubes with a micronic cap sealer. This is a simple table top device... device that has a roller to go over caps and push them into micronic tubes instead of pushing them in...

  17. Hot Plasmids February 2024

    Type
    Blog Post
    ...10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with ..., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier... macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters... for primary cells Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains... an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression...engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in ... primate (NHP) brains.  Systemic gene delivery capsids previously identified in rodents have not been ...

  18. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...Pipette 0.5 mL of Tween-20 to into 999.5 mL 1X PBS Cap the bottle and invert several times to mix. Carefully...) as follows: Add 250 mg of BSA to 25 mL of PBS. Cap the tube and invert several times to mix. Using a...PBS) as follows: Dilute 50 mg BSA into 50 mL PBS . Cap the tube and invert several times to mix. Dilute ...starting with three concentrations between 1–10 µg/mL. Cap and invert the tubes several times to mix. Using ... concentrations in the antibody dilution buffer. Cap and invert the tube several times to mix. Using a...mL Peroxide Solution from the TMB substrate kit. Cap the tube and invert several times to mix. Using a...

  19. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Transfer 6 mL of BCD TFX into each of two 50 mL tubes. Cap the tubes and incubate for 1 h in the 37 °C bead ...antibody plasmid DNA to one tube of 6 mL BCD TFX. Cap the tube and vortex for 5 s to mix. Add 450 µL of.../mL stock solution of PEI-MAX, 450 µg = 450 µL). Cap the tube and vortex for 5 s to mix. Pro-Tip The optimal...1:6. Add the diluted PEI-MAX to the diluted DNA. Cap the tube and vortex with three 1 s pulses. Incubate... 12 mL of PEI-MAX/DNA mix to the flask dropwise. Cap the flasks and swirl 5–10 times to mix. Return the...

  20. Viral Production

    Type
    Collection
    ...plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral helper sequences...intensity of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction...the relative stoichiometric ratios of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations...non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to...

  21. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...tubes. Cap the Gravitrap columns at the bottom. Add 5 mL of Pierce IgG Elution Buffer to the capped Gravitrap...containing 50 µL 1 M Trizma hydrochloride pH 9.0. Cap the tubes and vortex for 5 s to mix. Determine the...Spin Desalting Column’s bottom closure and loosen cap. Place column in a 50 mL conical collection tube....columns have a finite binding capacity. If your sample exceeds the capacity, divide the sample among multiple...1 part tissue culture supernatant and mix well. Uncap the Protein G Gravitrap or rProtein A Gravitrap ...columns. For information about the total binding capacity for your columns, refer to the manufacturer’s ...Gravitrap column and incubate for 10 min at RT . Uncap the column and collect 500 µL fractions into the...

  22. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...only 1.5 ml of each are used). This solution is capped, swirled and tested for the correct temperature...and not spread out onto the plate. We test the (capped) 1:1 agarose/DMEM solution against a part of the...

  23. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...Translation in eukaryotes usually begins at the 5’ cap so that only a single translation event occurs for...spark translation initiation independent of the 5’ cap.  IRES elements are very useful and commonly found... viral transfer vectors with limited packaging capacity. Additionally, it may not be feasible to express...

  24. Deck the Lab 2022!

    Type
    Blog Post
    ..._fang) That’s right, researchers of the world. Cap your Eppendorf tubes, hang up your pipettes, and ...

  25. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...(top) and with growth (bottom) Loosely close the cap on the bottle (do NOT close all the way or the bottle...cover the culture with sterile aluminum foil or a cap that is not air tight. Incubate bacterial culture...specific plasmid. However, a liquid culture is capable of supporting a higher density of bacteria and ...

  26. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TALENs Full Target Site (5' to 3'; half-sites in CAPS, spacer in lowercase) aak1 TAL3208 & TAL3209 TCTTTGACTCCCGGAGAGagctggtgggctccgggCCAGGTTCTGGAGGGGGA...

  27. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...last base pair with an end-capping loop (Nishimasu et al., 2014). This cap is where the DNA:RNA heteroduplex...lobe has been hidden for visibility. A RuvC loop caps the heteroduplex and marks the transition back to... cuts.   Pro tip! X-ray crystallography cannot capture flexible or disordered regions, so crystal structures...

  29. AAV ddPCR Titration

    Type
    Protocol
    ... drops along the side of the tube. Cap gently - do not push the cap in all the way, just ensure the samples...PCR tubes. Pipette back and forth 5 times. Lightly cap the PCR tubes. Generate the Droplets Bring the PCR...Without disturbing the liquid in the tube, gently uncap the PCR tubes. Add 5 µL of dilutions 6–8 to the ...Without disturbing the liquid in the tube, gently uncap the tubes. Add 5 µL of the previously aliquoted ...

  30. AAV Production in HEK293 Cells

    Type
    Protocol
    ...such that the media goes toward the cap. If the media touches the cap, replace it with a new one before ...optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing your gene of interest Triton...bp) DNA Concentration (μg/μL) Volume of DNA (μL) RepCap 7,265 1.00 727.6 pHelper 11,854 1.00 1,185 Transfer..., for each plasmid we need: Sample Calculations RepCap: 0.08 μg/bp × 7,265 bp = 582.1 μg Volume Needed...

  31. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...Actin, cyan), with formin (649-mDia1, magenta) and capping protein (549-CP, yellow) at the tip. 649-mDia1 ...actin barbed end assembly by twinfilin, formin and capping protein. Nature Communications, 14(1), Article ... workflows you already use. Or you can really capitalize on the dye’s performance and use super-resolution...

  32. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and IRES-dependent ECFP-HA-His6...Gateway vectors) pCAP03-acc(3)IV - Facilitate the capture and expression of bacterial gene...

  33. Pouring LB Agar Plates

    Type
    Protocol
    ...colloid. Cover the opening of the bottle with its cap or aluminum foil (but do not make an air-tight seal...distrubtion of agar over the bottom of the plate. Cap each plate after pouring and stack as you pour. If...that way for ~10 min. This will allow any steam to escape from the autoclave and will cool your gel-mix slightly...

  34. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...arabinose, cAMP complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites...

  35. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...conjunction with the viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate... varying capacities. AAV vectors containing the AAV2 ITRs can be cross-packaged with capsids from multiple...., 2019), and different combinations of ITR and capsid serotypes vary in their gene transfer efficiency... 101: Pseudotyping Viral Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide...2023). AAV- based vector improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https...

  37. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...retroviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol Precursor protein... Psi (Ψ) RNA target site for packaging by Nucleocapsid. WPRE Woodchuck hepatitis virus post‐transcriptional... of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5 kb, but inserts bigger...

  38. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...showcases an improved design of the guide RNA that caps the 3’ end with a structured RNA motif. These engineered...cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for cellular Delivery... AsCas12f1 plasmids   Selective endogenous encapsidation for cellular delivery of RNAs By Mike Lacy The...genomic deletions of up to 10kb but it is also capable of coding concurrent small insertions to allow ...templated by the 3′ extension of the pegRNAs. PEDAR is capable of correcting larger genomic rearrangements as ...

  39. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...BSL-2 materials, you’ll need to use aerosol-tight caps, compatible with your centrifugation speed, to cover...

  40. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...phenomenon to the presence of terminal proteins capping the ends of linear double-stranded AdV genomes....vectors (Lukashev et al., 2016). The protein capsid encapsulating the genome is majorly composed of three ...large transgene packaging capacity. For instance, with a packaging capacity of about 6 kb, the 1st generation...AdVs have already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the...
Showing: 1 - 40 of 402 results