We narrowed to 1,052 results for: FER

... of luciferase found in Addgene's collection include: Firefly luciferase (Fluc) Renilla luciferase (Rluc...Click-beetle luciferases ( CBRluc and CBGluc , respectively), Cypridina luciferase , and Luciola luciferase . Each...CMV Dual secreted luciferase reporter (EF1α-Gaussia luciferase, CMV-Cypridina luciferase). Feng Zhang Return... Plasmid Collections Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression...Gaussia luciferase (Gluc) NanoLuc® (Nluc) The mammalian codon-optimized version of firefly luciferase is often... often referred to as Luc2. Other luciferases in our collection include the red and green Click-beetle...type of luciferase has advantages and disadvantages depending on the application, and different luciferases...

...and references, including gRNA design tools, CRISPR FAQs, and community forums.... CRISPR References and ...and Information CRISPR References and Information Addgene Resources CRISPR Software General Resources CRISPR...kits. CRISPR Software Sanger Indel Analysis ICE (Inference of CRISPR Edits) This open access tool from Synthego...opens in a new window) This tool helps design (10 different prediction scores), clone (primer design), and...inactivation, NHEJ, and HDR. Includes a wide range of reference genomes, including animals, plants, bacteria, ... (CBE) base editors. Includes a wide range of reference genomes, including animals, plants, bacteria, ...

...premises we can classify a plasmid preparation in 3 different ways: Transformation grade DNA Cloning grade ... our Plasmids 101 blog posts Learn about the differences between transformation, transduction, conjugation...Resources on Addgene.org Read the molecular biology reference Find plasmids for your research Brush up on molecular...

.... This is why they are called transferable skills. Why are transferable skills so important? It is important... just technical skills, but also transferable skills, before offering someone their dream job; They want...   What is a transferable skill? Time management. I needed it when balancing a handful of demanding courses...useful anywhere I work in the future; they are transferrable across most, if not all, industries and work...important to identify which transferable skills you possess because any potential employers, collaborators...certainly matters in the job market, it is the transferable skills that create a full package and add an...an extra shine to your work. Knowing your transferrable skills allows you to build on them even more, ...

...researchers learn many transferable skills while working in the lab and by offering advice on areas where...feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this...where you might need some help. Today in our transferable skills guide: Negotiation. As a grad student,...I should negotiate my salary after receiving an offer but even as I did (successfully!), the process felt...how to spend my grant money (Can I attend this conference?) and with my lab mates on which lab jobs needed...done (Who is responsible for making the running buffer this week?). Scientists negotiate all the time ...Hawaii?). So how can you approach improving this transferable skill? Negotiation as a zero sum game Some ...

...codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the ... creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase by Promega’s Advanced ...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not...or firefly luciferase into mouse livers followed by i.v. injection of furimazine or luciferin 24 hours ...coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce...products at Promega.     References 1. Hall, M.P., et al. (2012) Engineered luciferase reporter from a deep...isn’t science better when we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...Chiara, the tech transfer expert who understands the somewhat complicated Material Transfer Agreement process...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...such an association, start one! These teams can offer a sense of solidarity you’re going to crave. The... - with the possible exception of when Thomas Jefferson dined alone.” There was a time in human history...could know a little bit about everything or in Jefferson’s case, even a lot about everything. Fast forward... a few dozen lab meetings. The lab, as a team, suffered for it. Nobody knew what anyone else was doing...

...codon-optimized 613 (luciferin),730–743 (analogs) 64 Luciferin or naphthyl-luciferin analogs ATP No Deep...Well-established Natural and engineered luciferases Although firefly luciferase was the first bioluminescent reporter...cruciata), and more. These variant proteins offer different kinetics, substrate requirements, and photon...have engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...Oplophorus gracilirostris) luciferase and engineered to be extra bright. Other luciferases have been modified ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...ATP No Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was first...

...Blog Transferable Skills Guide: Problem-Solving Transferable Skills Guide: Teamwork Transferable Skills...recognize their MANY transferable skills learned while working in the lab and offering advice on areas where...the lab can be applied anywhere! References and Resources References Grant, A. (2017). Originals how non-conformists...feel they lack formal training in a variety of transferable skills. At Addgene we're helping to fill this...Today, we’re highlighting an oft-underlooked transferable skill: creativity. I’ve heard quite a few scientists..., it’s clear that creativity is absolutely a transferable skill developed during PhD training. Creativity...inclusivity. Work with and value people who think differently from you and you will arrive at unique solutions...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...in mind! References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable skills and...Page Read Other Posts in the Transferable Skills Guide Developing Transferable Skills During Science Training...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...where you might need some help. Today in our transferable skills guide: Collaborating with others outside...collaborators from other institutions personally on conference calls and make sure to maintain these relationships... learning from others and meeting people with different mindsets and approaches can truly broaden your...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...test:  my undergrads were different people! More specifically, they had different learning styles. One was...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...where you might need some help. Today in our transferable skills guide: managing a team. You’ve just been...motivates them (keep in mind that it can be very different from what motivates you!), know their unique skills... strengths (you will likely have reports with different levels of experience), find out what their learning...will take responsibility (within reason!).  Try to buffer them from unnecessary pressure “coming from above...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...where you might need some help. Today in our transferable skills guide: Time Mangement for Scientists. ...tasks. If you’re constantly switching between different projects, you might not be able to make any reasonable...components: A mechanism for blocking off time for different tasks. I use google calendar for this and try ...

...Before the conference Is the conference you are attending on the larger side? At large conferences, chances...once in different rooms. Before arriving at the conference, plan out your agenda. Some conferences even ... the entire conference, in general). After the conference Review your favorite conference moments on social...present your findings at a conference. But what you get out of a conference depends on what you put into...introduction. During the conference When you meet someone new at a conference, one of the first things... the conference, you can end by asking them to come to your poster or talk. After the conference After...poster session Before the conference If you’re presenting a poster at a conference, be sure to practice beforehand...

... of the conference Addgenies were lucky enough to meet hundreds of the over 11,000 conference registrants...Check Out Our Microbiology Page! The start of the conference After setting up the Addgene booth on Thursday...involved with was one of the highlights of the conference. It was inspiring to see how microbiology research...out many of the posters being presented at the conference and talked to some scientists who stopped by ...from the nearly 4000 posters presented at the conference, it looks like there are plenty more exciting...Engineering Learn how to Network at Scientific Conferences Resources on Addgene.org Check out Our Microbiology...

...resources on the Addgene blog Transferable Skills Guide: Time Management Transferable Skills Guide: Conflict ...of being a scientist and an incredibly useful transferable skill. Being able to stand up in front of an...screeching feedback. You’re muted on Zoom. Your video conferencing software freezes and then crashes. You’re muted...remember to breathe!)   Public speaking as a transferable skill The worst thing about doing public speaking...most difficult form, which makes it the perfect transferable skill. Almost every other form of presenting... a clear, informative presentation, speak at conferences, or even just provide quick updates during a ...Conflict Resolution Transferable Skills Guide: Creativity...

...also matters what they’re stored in - their buffer. Buffers are made of chemicals, which have an unfortunate...the wrong everything else.      References and Resources References Boston University Flow Core. Fluorochromes... In this post, we’ll touch on antibody storage, buffer considerations, and give just the lightest of nods...standard curve, another quick experiment, or using a different vial and saving the poor, abandoned antibody for...their antibodies out. It's okay, Blugene!    Buffers  You know what temperature to store your antibodies...conjugation reaction to understand if any of your buffer components could affect the reaction. For instance...sodium azide, a common antimicrobial agent added to buffers, can bind to amine groups present in some conjugates...

...actually been a meeting? Different teams frequently prefer to communicate in different ways, so the source ...arbitrate for them. They were different people with some very different opinions, and some of them would...thomas-kilmann-conflict-resolution-model/    References and Resources References Thomas-Kilmann Conflict Model, Management... blog Management for ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork...without my “neutral” presence to alleviate those differences, they had a hard time maintaining their friendships...the right time. Like many of the posts in our Transferable Skills series, this post is not meant to be ... that you could strengthen. The right set of transferable skills, coupled with your technical skills, ...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this...where you might need some help. Today in our transferable skills guide: Problem-Solving. I don’t know about... careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined. In this ...resolves the issue.  If this sounds like about seven different skills to you, you are not wrong. Problem-solving...your life. This tactic is a good way to see how transferable these skills really are, while also practicing...Paired with your other skills, both technical and transferable (i.e. teamwork and time management), your ability...

...Research" conference. You can register for the conference here. The Future of Research conference is organized... insufficient (or no) emphasis on developing transferable skills to enable pursuit of these diverse opportunities...

... attend conferences. Depending upon your discipline, there are different types of conferences that you...organizations that hold large, annual conferences. Gordon conferences offer more focused and discipline-specific...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...experiences, and several Gordon conferences are held every year in different locations all over the world...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...were so critical to me landing my own position! Conferences One of the single most important things you can...world. But simply attending the conference is usually not enough. How do you stand out from the hundreds ...

...types in the body from otherwise terminally differentiated cells simply by expressing four proteins Oct4... usually I only get to scratch the surface of different fields. This meeting has given me an opportunity...can often take the back burner at scientific conferences. In my nearly 20-year career in science, I have...

...gradually taken over as the preferred method for many labs. However, RNA interference (RNAi) remains steadfast...umbrella term for a number of different applications that involve different types of small RNA molecules...maybe you’ll consider using RNAi! References and Resources References Bartel, D. P. (2004). MicroRNAs. ...An oldie but a goodie — a phrase used to refer to something that has fallen out of fashion but is still...background, a handy “oldie” as you might say. RNA interference (RNAi) is, as the name might suggest, an RNA-mediated...most popular choices for gene silencing. RNA interference RNAi is a useful tool to investigate the roles...-RNA (miRNA) Short hairpin RNA (shRNA) Short interfering RNA (siRNA) Piwi-interacting RNA (piRNA) miRNA...

... only problematic word. How many different things do you simply refer to as a “tube”? We took steps towards... words, they were leaving these meetings with different interpretations of what we discussed and decided...scientists learn how to communicate their work differently based on their audience. How you talk about your...at a poster is going to vary depending on the conference you’re attending and who approaches you. You ...with professionals from other backgrounds at conferences or networking events. And if you’re a wet lab...fellow team member. But, at Addgene, we use seven different types of “plates” (Fig. 1). When you have to tell... they could in fact be interpreted in several different ways depending on the team. When Tony, our corporate...

... the conference, map out which posters you are interested in viewing. For most larger conferences, poster...ensure you have the most beneficial scientific conference experience possible. Tips for poster session ... times while standing at your poster – it’s a different experience to practice while seated or with your...Dress for the venue and professionalism of the conference, and remember to wear comfortable shoes, as you...this, and have many times answered a completely different question than the intended one. Take a deep breath...tips and tricks for presenting at scientific conferences, so let us know your own ways to successfully... this blog post for tips on other aspects of conferences Resources on the Addgene website Become of ...

...second day of the conference included workshops which were framed in advance by the conference organizing team...the amazing, postdoc-driven Future of Research Conference at Boston University last week.  This fantastic...

... in a nonacademic direction, there are many “transferable” skills you have developed in addition to learning...your favor? Look for opportunities to practice transferable skills in ways that will also enhance your science... concrete things you can do to develop those transferable skills while you are also learning to be an ...colleagues you help will be future advocates and references. Collaborate with another lab, in another field... a committee and do something big like plan a conference Recruit many supporters and mentors that will...skills too. Teach classes Go to workshops and conferences on interviewing, resume writing, and networking... transferable skills...

...repeats (ITRs) are what makes an AAV transfer plasmid an AAV transfer plasmid. ITR sequences are 145 bases...Most AAV transfer plasmids have a promoter, but this element isn’t required when the transfer plasmid ...proteins needed for the virus to replicate,  and the transfer plasmid which contains the viral genome. In today... today’s blog post, we’ll focus on the AAV transfer plasmid and take a look at each of its parts. ... Figure 1: Parts of an AAV transfer plasmid. Parts shown in green are key elements of an AAV plasmid...structure and high GC content, but some companies now offer Sanger sequencing conditions which can read through...species or location. Promoters can also drive different levels of expression. For example, CAG is a strong...

...spending on a conference? One thing to consider is the size of the conference. Large conferences have many...make new relationships than at scientific conferences. Conferences provide the opportunity to meet people...especially when solving problems.  During the conference The conference may start in the airport. If you see ...programs. After the conference Follow up with the new people you met at the conference. If you promised ...overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups to connect...sessions that match your interests. I favor small conferences where the attendees stay onsite and eat together...face it, you see enough of them).  Before the conference Make sure you have a business card – even students...

...enzymes and these different Dicer-like enzymes are involved in the biogenesis of different small RNAs (Figure...plants, different dicer-like enzymes (DCL) process the dsRNA into specific types of interfering RNAs. ...that function downstream of DNA, such as RNA interference, can reversibly exert their effect independent...independent of gene copy number. RNA interference (RNAi) is a conserved eukaryotic process where approximately...equivalently precise, but each method recovered different genes known to be required for viability (Morgens...subsequent two. Consequently, I will focus on different approaches and considerations present at the first...been extensively used in plant research and many different constructs are available to the community. Each...

...sessions The poster session from conference to conference can be wildly different. There can be no presenter...participants used the conference hashtag after the conference was over. Depending on the conference, organizers ...scientific conferences. Plan your sessions, not your walks between sessions Like many large conferences with...can use to help plan your conference experience.One of the biggest differences: you don't have to build..., requiring you to zoom in to different parts of the poster. Conferences, such as Photonics Online Meetup..., 2020 Final thoughts on virtual conferences Virtual conferences remove many barriers of participation...spend with the conference. “If you want to have the same experience as an on-site conference, you really ...

...is redifferentiated to a different cell type. Transdifferentiation In this process, differentiated adult...reversal of a differentiated cell type to an undifferentiated state and then redifferentiation into the cell...variety of plasmids for differentiation here. Transdifferentiation Dedifferentiation to an intermediate pluripotent...ability to convert a differentiated cell to a different cell type. While transdifferentiation is suited for ...ReprogrammingA differentiated somatic cell reverts back to a pluripotent state, which then proliferates and is... are converted to cells of a different lineage, without dedifferentiating into a pluripotent stage. Cellular...pluripotent state. Redifferentiation - Conversion of the pluripotent cells into differentiated cells of choice...

...supporting in-person conference attendance, the award now supports virtual conference attendance. We also...Michael Davidson and Roger Tsien Commemorative Conference Awards. Congratulations to the five winners! ...awards help early career researchers attend a conference of their choice while honoring the late Michael...and highly interactive environment that Janelia offers,” says Aggarwal.In addition to the biosensors, ...hydropersulfide-propagating enzymes 3-mercaptopyruvate sulfurtransferase (MPST) which catalyzes the desulfuration of...an enzyme-bound hydropersulfide that is then transferred to thiol-containing acceptors. These hydropersulfides...redox modification, the MPST hydrosulfide can be transferred to the roGFP2 cysteine thiols and change its ...

...scientific network We refer to the process of mentoring within a bin as Horizontal Transfer. This comes from...from horizontal gene transfer, which occurs when bacteria of different evolutionary lineages share genes...Goldschmidt conference, Coastal & Estuarine Research Federation (CERF) Biennial Conference, Gordon Research...Science’s (SACNAS) annual conference all foster mentorship in different but successful ways. Scientific ...Binning Singletons. The sheer scale of a large conference can be intimidating. And it can be exacerbated...other and what to do.  Saved by the Tweet - The conference hashtag I had, however, started a Twitter account...like anyone at a meeting who felt isolated at a conference for any reason was a Singleton. Figure ...

...interest. They also “showed that different guide RNAs have different abilities to turn genes on and off..., transformations require electroporation. References & Further Reading: For more information about...

...comes the need for new conference room names. The Addgenie’s favorite conference room naming scheme? Gene...largest conference room at Addgene is named Sonic Hedgehog but this is not a direct reference to the video...community. Not surprising, this is where most of our conference room names originated from. Let’s take a look...Groucho, a transcriptional co-repressor The first conference room, that is actually used as a break room for...diffuses down a concentration gradient exerting a different effect on the developing embryo depending on its...Surprisingly, Sleeping Beauty is not only a princess who suffered a terrible fate when she pricked her finger on...required for spermatogenesis One of our smaller conference rooms is named Cookie Monster. cookie monster...

...IFNA10 interferon, alpha 10 MGC119878, MGC119879 IFNA13 interferon, alpha 13 - IFNA14 interferon, alpha...IFNA16 interferon, alpha 16 - IFNA17 interferon, alpha 17 IFNA, INFA, LEIF2C1 IFNA2 interferon, alpha ...MGC142200 IFNA5 interferon, alpha 5 INFA5 IFNA6 interferon, alpha 6 - IFNA7 interferon, alpha 7 IFNA-J IFNA8...

...cells are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...Research Reprogramming Plasmids Differentiation and Transdifferentiation Plasmids Stem Cell Research Plasmids...can be directly differentiated into specific somatic stem cells or fully differentiated cell types. Browse...collection of Differentiation Plasmids below. Alternatively, it is also possible to directly differentiate one ...one differentiated somatic cell into another via transdifferentiation. This method of cell lineage conversion... of Transdifferentiation Plasmids below. Many of the techniques for reprogramming, differentiation, and... , SOX2 , MYC , KLF4 , LIN28 . Differentiation and Transdifferentiation Plasmids Browse the table below...

... dates. Buffers Buffers are a concern for conjugating and signaling molecules, as some buffer components...heavy chain constant region” or Fc. Different isotypes play different roles in the immune response, and ...removed from the buffer before the reaction. However, it can be added back to buffers after the conjugation...of colors activated by different wavelengths, allowing for easy differentiation between multiple antibodies... In some cases, it may be preferable to buy small aliquots of many different antibodies and do batch validation...an antigen, while the specific binding site is referred to as an epitope. Production Antibodies are typically...typically produced in one of three different methods: in animals, in cultured immune cells, or through...

...many different Cas proteins available, and researchers can adapt these experiments for different organisms...Desired Genetic Manipulation Different genetic manipulations require different CRISPR components. Selecting...editor variants often have different optimal editing windows. Be sure to refer to the literature associated...Activation/interference Needs good primer design Western blot Knockouts Activation/interference Needs a ...Expression and Delivery Validate Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S...have expanded the CRISPR toolbox to include many different types of edits, including point mutations, base...Base editor (dCas9 or Cas9 nickase fusions with different base editing proteins) Many base editors fused...

... CMV ALS Shawn Ferguson 83439 pSpCas9(BB)-2A-Puro-C9orf72 C9orf72 U6 ALS Shawn Ferguson 83441 pSOD1E133insTT-AcGFP1...TARDBP CMV ALS Shawn Ferguson 133754 pLVX-Puro-TDP-43-A90V TARDBP CMV ALS Shawn Ferguson 133755 pLVX-Puro-...TARDBP CMV ALS Shawn Ferguson 133756 pLVX-Puro-TDP-43-A382T TARDBP CMV ALS Shawn Ferguson 133757 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133758 pLVX-Puro-TDP-43-K263E TARDBP CMV ALS Shawn Ferguson 133759 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133760 pLVX-Puro-TDP-43-P112H TARDBP CMV ALS Shawn Ferguson 133761 pLVX-Puro-TDP...TARDBP CMV ALS Shawn Ferguson 133762 px459-TDP-43 sgRNA1 TARDBP U6 ALS Shawn Ferguson 133763 px459-TDP 43...Suzanne Pfeffer 186013 pCMV Flag-LRRK2 K17A/K18A/R1441G LRRK2 Flag CMV Parkinson's Suzanne Pfeffer 186016...

...Resonance Energy Transfer (FRET) is a process by which energy is non-radiatively transferred from an excited...energy transfer mCerulean, mVenus, and Amber (mVenus Y67C) constructs for measuring energy transfer to multiple...Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection or the Promega...FLIM) or FLIM-FRET, where the increased energy transfer through FRET reduces the donor lifetime. FRET ...separately fused to a donor or acceptor molecule (also referred to as intermolecular or bimolecular FRET) or (...acceptor are both fused to the same protein (also referred to as intramolecular or unimolecular FRET). Learn...factors. When designing your experiment, be sure to refer to published reports and include appropriate controls...

...nonprofit, Addgene offers expert support, educational resources, and efficient material transfer solutions that...HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter...NanoLuc luciferase-fusion protein expressed in cells. Additional Resources Browse Addgene's Luciferase Plasmid...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...experimental workflows. The collection contains luciferase reporter vectors for sensitive gene expression...bacteria as an agar stab 20 µg purified DNA in Tris buffer (500 ng/µL ± 25 ng/µL) All plasmids in the collection..., reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "NanoLuc", "HaloTag", "BiT", or other...

...Alternatives Resources Plan Your Experiment Web References PAM Sequences Glossary Publications CRISPR Overview...techniques . Collectively, these techniques are referred to as CRISPR ( C lustered R egularly I nterspaced...measure of fidelity, so sometimes they will be referred to as increased fidelity enzymes. hfCas9s were...pyrimidine-to-pyrimidine) Base transversions — edit to different classification of nucleotide base (purine-to-pyrimidine...opposite strands Prime editing and base editing both offer high efficiency and low indel formation compared...genetic changes, including moving entire genes to different locations, by combining other gene editing mechanisms...DNA-targeting of CRISPR-Cas systems. You may also see them referred to as INTEGRATE ( I nsertion of T ransposable ...

...pOpen-EcoRIM M.EcoRI DNA methyltransferase 165517 pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM...pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM...pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI 165538 DNA methyltransferase 165547 pOpen-SapIM1...pOpen-SapIM1 M1.SapI DNA methyltransferase 165557 pOpen-SapIM2 M2.SapI DNA methyltransferase 165558 pOpen-EcoRVM...pOpen-EcoRVM M.EcoRV DNA methyltransferase 165577 pOpen-XbaIM M.XbaI DNA methyltransferase 165578 pOpen-NcoIM ...pOpen-NcoIM M.NcoI DNA methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red...red operon 165530 pOpen-T4-BGT T4-BGT beta-glucosyltransferase 165531 pOpen-UDG UDG Uracil DNA glycosylase...

...pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase 3rd Expression of EGFP-Firefly luciferase fusion protein. Ethan Abel...envelope, and transfer plasmids Viral... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Transfer Plasmids Packaging...a virion) are divided among multiple plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA ...third-generation lentiviral packaging, envelope, and transfer plasmids available at Addgene. Read our Lentiviral...safety. Lentiviral Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express... 3rd U6-driven shRNA empty plasmid. Includes a stuffer for easy cloning. David Root 14748 pLKO.3G 3rd ...

...listing of transfer status for viral preps.... Viral Vector Packaging Service Penn Vector Core Transfer Viral ...Viral Vector Packaging Service: Penn Vector Core Transfer The University of Pennsylvania Vector Core (Penn...inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including...mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-50943 50943-...mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68717 68717-...pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener, Tobias Rose AV-1-68719 68719-...

...to LacZ, plus luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase...plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase...Library CRISPR page for more information. Full references are below. Return to top Cas9 SAM transcriptional...The MS2-P65-HSF1 activation helper protein Full references are below. Pooled genome-wide human SAM library...Swiech et al., Nature Biotechnology 2014). Full references are below. Available plasmids are described below...The vector can be digested using BsaI ( This is different from BbsI, which is used in the SpCas9 plasmid...with Cas9 in a wide range of applications. Full references are below. The Cas9 knockin mouse can be purchased...

...Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors Resources Kazuhiro Oka and...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of...packaging these transfer vectors and generating new viral vectors. Download the Lentivirus Transfer Vector Production...from the PGK promoter Adenoviral Transfer Vectors The adenoviral transfer vectors contained within this ... of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre 72256 Expresses copGFP...Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors pICPIS-EF1 (Addgene...

..., 5 Ofer Yizhar 198511 pAAV_hSyn-DIO-PdCO-mScarlet-WPRE Syn PdCO mScarlet Cre dependent 1, 5 Ofer Yizhar...GtACR2 (soma-targeted) FusionRed Constitutive 1, rg* Ofer Yizhar 105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed ...soma-targeted) FusionRed Cre dependent 1, 5, rg* Ofer Yizhar 105678 pAAV_hSyn1-SIO-stGtACR1-FusionRed ...GtACR1 (soma-targeted) FusionRed Cre dependent 5 Ofer Yizhar 109048 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1...mScarlet-WPRE CaMKII eOPN3 mScarlet Constitutive 1, 5, rg* Ofer Yizhar 125713 AAV-hSyn1-SIO-eOPN3-mScarlet-WPRE ...mScarlet-WPRE Syn eOPN3 mScarlet Cre dependent 1, 5 Ofer Yizhar 139504 pAAV-CAG-DIO-PPO-Venus CAG PPO Venus Cre dependent...pAAV_hSyn-PdCO-EGFP-WPRE Syn PdCO EGFP Constitutive 1, 5 Ofer Yizhar 198516 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...

...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...Tools for RNAi Browse Plasmids Resources RNA interference (RNAi) is an RNA-mediated gene silencing mechanism...as shRNA (short hairpin RNA) or siRNA (short interfering RNA). Delivery can occur via transfection or ...using viral vectors. Lentiviral vectors are a preferred method for long-term gene silencing. Once the ...below. Figure 1: Overview of shRNA-mediated RNA interference. Created with BioRender.com. shRNA Plasmids ...pSico with pSicoligomaker (Andrea Ventura lab) References Carthew, R. W., & Sontheimer, E. J. (2009). Origins...J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews, 61 (9), 746...