...plasmids for metabolic enzymes and pathways.... Plasmid Collections Synthetic Biology Metabolism Synthetic ...Synthetic Biology: Metabolism SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome...Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation Sensing & Signaling Strains...collection of synthetic biology plasmids related to metabolic pathways and components. Examples Include: Biofuels...Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table by keyword or sort ...

...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...protein of interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform...basic protocols and controls follow that of IF (above). Sample preparation, signaling excitation, and ...

...information about the type of genetic modification and target species. Additional information about each library...Activation Human Zhang 3rd 10 96,458 Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini...Human Vakoc 3rd 6 3,051 Human Lipid Droplet and Metabolism Library 191535 Knockout Human Olzmann 3rd ~10...Yusa 3rd 5 90,230 Mouse Lipid and Sphingolipid Metabolism Knockout Libraries 206248 206249 Knockout Mouse...Mouse Birsoy 3rd 7–10 2,105 630 Mouse CRISPR Metabolic Gene Knockout Library 160129 Knockout Mouse Birsoy...Inhibition Yeast Tavazoie N/A 6-12 51,681 Mouse Metabolism Library 163966 Knockout Mouse Kimmelman 3rd ~...Knockout Mouse X.S. Liu 3rd 5 5 10 49,252 Human Metabolic Gene CRISPRa sgRNA Library 187080 Activation Human...

...the Pertz laboratory to construct custom biosensors. The ScEnSor Kit from the Olsson laboratory includes...sensors for live-cell imaging Live cell imaging of metabolic heterogeneity by quantitative fluorescent ATP ...protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion profiling. ...David Savage Maltose Monitor maltose levels and metabolism (FLIPmal) Fluorescence Resonance Energy Transfer...Biosensing Reveals the Dynamics of Cytosolic Redox Metabolism in Plants. Plant Cell. 2020 Aug 13. pii: tpc.20.00241...concentration Semisynthetic sensor proteins enable metabolic assays at the point of care. Science. 2018 Sep...biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion profiling. ...

...Addgene's collection of KLF plasmids and learn more about the scientists who have created and shared these...Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About The Krüppel-like factors (KLFs) are a family of ...proliferation, differentiation, inflammation, metabolism, fibrosis, atherosclerosis, and carcinogenesis...transcriptional activation and/or repression. Continuing collaborative efforts within the KLF community have lead to...to contribute to this community resource. Learn about the benefits of depositing plasmids with Addgene...

...integrating MDH-related research into their teaching laboratories. They encourage instructors teaching different...research community and facilitates scientific collaborations between institutions. With these plasmids, ...regulation and post-translational modifications, and metabolic pathways and protein interactions. Plasmids A ...projects, find teaching resources and protocols, learn about regional hubs, access a teaching mentor, and more....2021.43 (Link opens in a new window) External Collaboration Results in Student Learning Gains and Positive...

...reporter, and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components...Learn more about synthetic biology resources available at Addgene. Browse featured collections or depositing...Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas...Schmidt-Dannert Pamela Silver Lei Stanley Qi Jeff Tabor Jan-Willem Veening Christopher Voigt Clifford Wang...

... the cell and regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein...mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many...ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation...energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome biogenesis...

...in vitro ? These, in addition to many questions about bacteria themselves, can be answered using model...overexpressing or knocking down a gene can tell you much about its function and/or alter cellular activity in a...basic biology of a bacterial gene or redirecting metabolic pathways to produce a therapeutic compound, there...once you get it right, you can learn many things about your protein’s function, structure, and interactions...molecules, light, temperature, or even intracellular metabolite levels. Different plasmids and, often times, ...pCph8 50552 pLtetO-1 Anhydrotetracycline Jeffrey Tabor Anhydrotetracycline inducible expression of red ...

...Previous Article: Changing Labor Laws Bring Increased Postdoc Wages Read about the Advocating for Science... of $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This was due to the threshold...postdocs - and in some cases, faculty - have found out about what their institution is doing from Future of Research...Science Symposium and Workshop Learn about a Variety of Science Careers Resources on Addgene.org Find Career...

...spectral barcodes for cell tracing - Sean Megason Lab Zebrabow - Albert Pan and Alex Schier Labs. Multicolor ...zebrafish strains and materials and information about zebrafish research. China Zebrafish Resource Center...America. The Zebrafish Book - A guide for the laboratory use of zebrafish. CRISPRz - A database of CRISPR...gene expression database that provides information about when genes are active in developing embryos. zfRegeneration...

... Frederick National Laboratory for Cancer Research . Frederick National Laboratory for Cancer Research...

...resource is a collaboration between the Cell Migration Consortium and Addgene. Member laboratories of the Cell...This resource is a collaboration between the Cell Migration Consortium (CMC) and Addgene, which highlights...

... information about: γ-Retroviral packaging systems Frequently asked questions (FAQ) about retrovirus Popular...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...

...Learn about Addgene's 2018 AAV Education and Development (AAVED) workshop series.... Institute Katherine Matho Cold Spring Harbor Laboratory Gowan Tervo Janelia Research Campus Sarada Viswanathan...Institute Katherine Matho, Cold Spring Harbor Laboratory Gowan Tervo and Sarada Viswanathan, Janelia Research...

...Virus Should I Use? Use this guide to learn more about the different types of viruses that are commonly...temporary in the host. Use this guide to learn more about the different types of viruses that are commonly...found in the guides for each virus type, linked above. Virus Expression Genome Packaging Capacity Virus...

... Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd generation...systems Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral... or shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono...

...Find out about the plasmid collections available at Addgene that may be useful for neurodegeneration ...Scientists are studying protein aggregation, learning about the blood brain barrier integrity, and using CRISPR...with support from the BRAIN Initiative. Jackson Laboratory (Link opens in a new window) A collection of ...

... working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid resource...contact information by following the link to their laboratory website. Mouse Line Mutation (DNA) Background...contact information by following the link to their laboratory website. Cell Line Mutation (DNA) Mutation (protein...

...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable...when carrying out CRISPR experiments. Read more about how to design your gRNA . Browse Plasmids: gRNAs...to optimize your delivery conditions. Learn more about CRISPR delivery in mammalian systems . Validate ...

...Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids from 2016 blog post...circularly permuted Venus fluorophore. Learn more about pHlash sensor in our 28 Hot Plasmid Technologies... with the original STARR-seq method. Learn more about STARR-seq in our 27 Hot Plasmids from 2016 blog ...

...and are discussed in more detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA...Nme2Cas9 (from Neisseria meningitidis ), which are about the same size as SaCas9, with higher fidelity than...targeting range for plant genome engineering. Read more about Cas9 variants . Anti-CRISPR Prolonged Cas9 activity...convert one base to another within a target locus of about 8 bp, specified by a gRNA. There are two main types...sequence may not be characterized *In the table above, 3' and 5' indicate on which end of targeted sequence...

...Function (IGVF) Consortium Collection Read More About... The IGVF Consortium in their preprint (Link opens... Research Institute (NHGRI) is funding this collaborative program that brings together teams of investigators...

...signaling network for transitioning between anabolic and catabolic states. Ras Ras is a GTPase that is essential...

...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... protein plasmids. Use this guide to learn more about the many applications of fluorescent proteins (FPs...

...Proteins for in vivo imaging have emission near or above 650 nm as signals below 650 nm are highly absorbed... interactions in vitro and in vivo . Learn more about in vivo imaging tools in this blog post - In Living...

...of cell surface and secreted proteins from a collaboration between Addgene and IPI....antibodies in an open science model. Learn more about IPI (Link opens in a new window) Glypican Antibodies...

...attachment site of the phage 186. Detailed information about the repression system can be found in this article...29519933 . bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be found in...

...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and...., Nat Methods 2013 ) More detailed information about REAL, REAL-Fast and FLASH, including detailed and...

...control data. Help Center Articles Additional details about viral vector production at Addgene, ordering information...receive your viral vector from Addgene, with details about storage. Biosafety Guide Documentation for your ...

...expression from the CAG promoter. For information about each catalog item, including the titer and quality...from the human synpasin promoter. For information about each catalog item, including the titer and quality...

... by email will supersede the general guarantees above. We will have our expert viral vector production...supersede the general estimated turnaround time above. After starting viral production, if we determine...

...important in cellular recognition processes. Laboratories interested in these approaches have created ... and secreted proteins Mouse sperm Mouse egg Laboratories using AVEXIS: Christian Sollner, Max Planck ...

...information about: Adenoviral components Adenoviral plasmid construction Frequently asked questions about adenovirus...

...nuclear receptor plasmid collection assembled in collaboration with the Nuclear Receptor Signaling Atlas (NURSA...associated with numerous disease pathways. In collaboration with research labs from around the globe, Addgene...

...quality control partner before distribution. Details about our production protocols, titering methods, and ...Depositing Lab on the antibody webpage. To learn more about these antibodies, please visit the Institute for...

...plasmids that are available to industry. Learn more about the available plasmids and how to order them....Information page for more information. To learn more about Addgene’s MTA process please visit our Help Center...

... system, fused to sfGFP for imaging. Learn more about antibodies and their applications in our Antibody...phage display vector system for isolation of scFv, diabody and Fab fragment libraries from chickens Methods...

...production. Read our AAV Guide for more information about: AAV components Common uses of AAV Viral integration...out our Viral Vector Packaging Service and learn about our options for hundreds of in-stock preps that ...

...synthetic operons for chloroplast transformation and metabolic engineering in plants (including tobacco and potato... opens in a new window) - Transcriptomic and metabolomic resources for 14 key medicinal plants for the...

...ROBO2 roundabout, axon guidance receptor, homolog 2 (Drosophila) KIAA1568, SAX3 ROBO3 roundabout, axon...adaptive immune cells, such as T cells and B cells. About a week following the initial exposure to a pathogen...eosinophil-derived neurotoxin) EDN, RNS2 ROBO1 roundabout, axon guidance receptor, homolog 1 (Drosophila...

... before being distributed to customers. Details about our production protocols, titering methods, and ...either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities, ...

...lightning speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV-...and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397...

...Protocols gRNA Design Tools CRISPR Blog Posts In collaboration with the labs who have deposited CRISPR plasmids...short list of genes provided by the user. Read more about it on the Addgene blog . Eukaryotic Pathogen CRISPR...

... Feng Zhang's Lab, has assembled a list of FAQs about using the lab's CRISPR technology...., February 2013 ), has assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang...

...serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations were produced...Janelia Research Campus viral project team in collaboration with David Schaffer (University of California...

...systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link opens in ...AAV Decision Tree The AAV decision tree graphic above helps you choose the best capsid for your cell type...

... other are collaborating on projects. When I was a graduate student, I led a collaborative project between...funding and circumstances permit, think about having collaborators spend time with each other in person....meet up with collaborators. Being able to present data from multiple aspects of a collaboration and immediately...the Addgene blog Read our blog post about external collaborations Browse our science career related blog...communication a non-negotiable part of your collaboration. Luckily, there are techniques and tools that...same page. Ideally, these meetings include all collaborators, from the lab members who are conducting the...interesting results or ask questions to your collaborators. This helps everyone feel included in the project...

...article from Journal of Visualized Experiments (JoVE) about genomic deletions in mammalian cell lines using ...up to 20 μl. Use the primers designed in step 2 above. Conduct PCR for “non-deletion band” and “deletion...

...guide: Collaborating with others outside your organization also known as external collaborations. Interestingly... and collaboration management. For example, you may already be part of a scientific collaboration - show...friendly, and helpful. Finally, don’t forget about collaborations and projects you can do with others after...lead! Read our blog post about fostering communication in remote collaborations! Stay in touch: Maintaining...notes that remind me about the current state of a partnership. Every collaboration and partnership is different...favored by research-intensive careers. External collaborations are becoming increasingly important as more...scientific project to the next level. External collaborations and partnerships are also very important in...