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  1. Video Library

    Type
    Protocol
    ...Resources Addgene Videos Addgene Videos Protocols How-To Videos Career Videos Browse Addgene's collection...using Addgene's resources, and career advice. For written lab protocol material, visit our Addgene Protocols...Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions for...samples from Addgene Technology Transfer How to Order A step-by-step walkthrough of Addgene's ordering process...this first installment of the Addgene Careers series, we sit down with Addgene Outreach Scientist Jessica...she loves about working at Addgene! Maria Soriano Maria Soriano, from Addgene's UK office, discusses her...plasmids with Addgene Depositing Page Career Videos Get career insight and advice from real Addgenies! (Link ...

  2. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...charge. What primers were used by Addgene during quality control? Addgene lists the primers used to obtain...and need to design a custom primer, Addgene recommends using Addgene's sequencing results as a reference...wish to contact Addgene about the accuracy of your plasmid, please email help@addgene.org and include: The...regions of your plasmid that you wish to confirm. Addgene's plasmid information pages recommend 5’ and 3’ ...a specific region of the insert. You can find Addgene's sequencing results by clicking on the "View Sequences...Sequences" link on the Plasmid Information Pages. Addgene sequences the plasmid to verify tags, mutations..., but we do not sequence the entire plasmid. Addgene strongly recommends that you sequence any portion...

  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...materials from Addgene to any party are governed by Addgene’s Terms of Use , Addgene’s Terms of Purchase...Cloning Vector Addgene Plasmid 10878. Protocol Version 1.0. December 2006. Copyright Addgene 2006, All Rights... from Addgene are recommended for use in conjunction with the pLKO.1 TRC-cloning vector. Addgene Plasmid...Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells GenHunter: #Q401...Information Addgene is committed to providing scientists with high-quality goods and services. Addgene makes...PubMed . H.2. Web resources Addgene’s mammalian RNAi website: www.addgene.org/mammalianrnai The RNAi Consortium...Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting...

  4. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ...designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational...administrators, or anyone else associated with Addgene, can be held responsible for your use of the information...

  5. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...using ready-to-use bio-safe Coomassie G-250 stain (Addgene uses SimplyBlue SafeStain) and ImageJ software....reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products... may not be functional. This sample would fail Addgene QC and would not be used. Section 3: Image Analysis...11469.296 4655.113 16642.522 16124.409 96.887% Pro-Tip Addgene only uses recombinant antibody preps that have ...

  7. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational...administrators, or anyone else associated with Addgene, can be held responsible for your use of the information...

  8. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Calculation Size in bp of Addgene plasmid #59462 : 6208 bp Concentration of Addgene plasmid #59462 : 1.07 ...AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol. Protocols...curve: One option is to use plasmid #59462 from Addgene. The values highlighted below in red were calculated...titer >3 x 10 13 GC/mL, use dilutions 5–8 Note: at Addgene, samples typically range from 1 x 10 12 GC/mL to...

  9. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning... Addgene Protocols On this page you'll find practical lab...column. You can find even more video content on the Addgene Video Page . We hope that you'll find these protocols...own work. Please feel free to email us at help@addgene.org with any questions. Intro to the Lab Bench Basic...

  10. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Plasmid DNA from Bacterial Culture All Addgene Protocols Addgene Resources Molecular Biology Reference ... antibiotic powder can be dissolved in dH 2 0. Addgene recommends making 1000X stock solutions and storing...

  11. CRISPR Library Amplification

    Type
    Protocol
    ...Molecular Biology Reference How do I process my Addgene pooled library? Introduction Please refer to our...depositor for a specific pooled library available at Addgene. We recommend following the depositor's specific...results. If you obtained the pooled library from Addgene, these protocols can be found on the Pooled Library...with ice and water to create slurry) 100 mL LB At Addgene we use premixed LB (VWR 101414-072) for convenience...Help Center to get details on how to process your Addgene pooled library...

  12. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...also like... Viral Vector Guides Virus Blog Posts Addgene Protocols Viral Service Introduction This protocol...concentration that was used for the initial selection). Addgene recommends that the use of selective medium throughout...cell lines. When this protocol was performed at Addgene on the A549 cells described in our sample data ... 1 lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors...

  13. Ligation Independent Cloning

    Type
    Protocol
    ...an example of LIC experimental design. Search Addgene's collection of empty LIC cloning vectors Protocol...transformation, following a standard protocol . Search Addgene's collection of empty LIC cloning vectors...

  14. Immunocytochemistry

    Type
    Protocol
    ...reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products...

  15. DNA Quantification

    Type
    Protocol
    ...your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol....

  16. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products...

  17. Western Blot

    Type
    Protocol
    ...reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products...

  19. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...reagents, and methods that we use in our lab at Addgene. Equipment and reagents from other vendors should...accommodate slight differences between products. Addgene does not endorse or recommend specific products...

  22. Pipetting Protocol

    Type
    Protocol
    ...consider using a multichannel pipette. Please watch Addgene’s protocol on multichannel pipetting to learn more...

  26. AAV ddPCR Titration

    Type
    Protocol
    ...control (NTC) should be close to zero (B08). At Addgene, we consider runs with an NTC > 10 to be invalid...

  27. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Fluorescence Titration Assay AAV Titration by qPCR Addgene Viral Service Lentivirus Guide Virus Blog Posts...

  28. Distribution to Industry

    Type
    Collection
    ...Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to...protection for material providers. Addgene's deposit agreements allow Addgene to distribute plasmids on behalf...A portion of Addgene's collection is available to qualified for-profit entities. Addgene reserves the ...can be submitted to Addgene through your user account or by email to mta@addgene.org . After we review ...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available...For target validation and drug design Antibodies Addgene distributes ready-to-use recombinant antibodies...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More...

  29. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Request: Scope of Service Addgene's Packaged on Request Service is subject to Addgene’s Terms of Purchase and...with our materials, Addgene cannot guarantee the function of any viral vector. Addgene performs QC measures...produced through our Packaged on Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL ... to toxicity or other reasons For these cases, Addgene will promptly notify you by email within a few ...not yield the titer guaranteed here or by email, Addgene will contact you to offer some options for moving...this analysis, you will be notified by email. If Addgene determines that our current queue is too high and...not expect to meet the general turnaround time, Addgene will notify you by email before, or within 10 business...

  30. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ... production at Addgene, ordering information, and more. Additional Resources Addgene's Data Hub Curated...general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from Addgene and scientists across the...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation ... Viral Vector Packaging Service Addgene offers flexible packaging options so you can easily...viral vector is produced from a cargo plasmid in Addgene’s collection. These plasmids are created by your... to do when you receive your viral vector from Addgene, with details about storage. Biosafety Guide Documentation... the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs of particular...

  31. All Antibodies

    Type
    Collection
    ...Browse Addgene's collection of recombinant monoclonal antibodies....Antibodies Addgene distributes ready-to-use recombinant antibodies. These monoclonal antibodies undergo application-specific...application-specific validation and quality control by Addgene as well as by our trusted partner labs. On this...antibodies currently available from our catalog. Addgene values transparency and accessibility in research...reactivity. Reactivity is based on validation data from Addgene or trusted partner labs or is predicted based on... on sequence homology with the antigen. Addgene supplies a list of recommended applications based on our...immunohistochemistry, and immunocytochemistry. Addgene will continue to work with both depositing and ...

  32. Viral Production

    Type
    Collection
    ...Production AAV distributed by Addgene has been produced either in-house by Addgene scientists or through collaboration...Browse AAV preps available from Addgene. After receiving your virus from Addgene, read our detailed recipient...lentiviral preps available from Addgene After receiving your virus from Addgene, read our detailed recipient...Overview of Addgene's viral production methods and quality control for lentivirus and AAV. Viral...are titered and subjected to quality control by Addgene before being distributed to customers. Details ... -80 °C. Titer Titering is either performed by Addgene or by the University of Pennsylvania Vector Core...which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet digital PCR...

  33. Antibody Production

    Type
    Collection
    ...Many antibodies distributed by Addgene have been produced in-house by Addgene scientists. They are generated...or by contacting help@addgene.org . Some of the antibodies distributed by Addgene were developed by the...Overview of Addgene's antibody production methods and quality control processes for purified recombinant...titered and undergo stringent quality control by Addgene or a trusted quality control partner before distribution... preparations developed at IPI may differ from Addgene antibodies in “Production & Usage” and “Delivery...identity. For more details on protocols see the Addgene Protocols page or the Neuromab Protocols (Link ...new window) page. Storage and Shipping Stability Addgene does not recommend repeated freeze thaw of antibodies...

  34. Bacterial Expression Systems

    Type
    Collection
    .... Additional Addgene Genome Engineering Resources Learn more about CRISPR with Addgene's CRISPR Guide ...are also being used for their distinct features. Addgene distributes many plasmids that can help researchers...MalE, OmpA ID Plasmid Promoter Tags PI Additional Addgene Protein Purification Resources The Structural Genomics...localization to the periplasm. The pCri System (Addgene #1000000058) plasmids can be utilized for heterologous...and Pichia pastoris . The EcoFlex MoClo Toolkit (Addgene #1000000080) features a library of promoters and...Gate modular cloning (MoClo) for use in E. coli . Addgene Blog E. Coli Strains for Protein Expression Protein...localization in live cells. ID Plasmid Tags PI Additional Addgene Protein Visualization Resources Visit our Fluorescent...

  35. Microbiology Resources

    Type
    Collection
    ...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses... Plasmids Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range ...additional microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library and distributions...regulations. Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for work in these model...genes or applications that are not listed here. Addgene Microbiology Resources Plasmid Kits for Microbiology... depositor protocols and gRNA design software. Addgene yeast CRISPR resources Bacterial CRISPR Resources...

  36. Antibodies

    Type
    Collection
    ...Overview of Addgene's ready-to-use monoclonal recombinant antibody collection. These affinity reagents... Antibodies Addgene distributes ready-to-use recombinant antibodies...experimental data for recommended applications. Addgene values transparency and accessibility in research...All Antibodies Ready to use antibodies? Browse Addgene’s collection of antibodies developed and validated...Production Interested in our production process? At Addgene, we provide transparency in our production process...antibody. Antibody Blog Posts Recipient Instructions Addgene Help Center Antibody Protocols Looking for Antibody...

  37. Viral Vector Guides and Plasmids

    Type
    Collection
    ...research purposes. Interested in Addgene's viral services? See Addgene's Packaged on Request service for...made-to-order AAV vectors from Addgene's AAV plasmid collection. Addgene also has a selection of in-stock... guidelines, check out Addgene's Biosafety Resource Guide . Resources Addgene’s Viral Vector Packaging...Packaging Service Addgene's Viral Protocols Addgene Blog Viral Vector Topics Page Viral Vectors 101 eBook Content... viral vector guides and plasmids available at Addgene and other related resources. Science...

  38. CRISPR References and Information

    Type
    Collection
    ... Information Addgene Resources CRISPR Software General Resources CRISPR Protocols Addgene CRISPR Resources...Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR ... and news published on the Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled...protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene Church ...Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR...activation, inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software...

  39. AAV Packaged on Request

    Type
    Collection
    ...experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and...this service. Select any eligible plasmid in the Addgene repository in your choice of seven serotypes and...more about AAV serotypes and tissue tropism on Addgene's blog . All-in-One Pricing The price for our Packaged...production Density centrifugation purification Addgene’s comprehensive quality control process: Titering...remember to share your protocols and results through Addgene’s Data Hub . By contributing your AAV vector experience...let you know whether the item can be packaged. Addgene does not perform functional or in vivo testing.... we produce high-quality viral vector preps at Addgene, including purification, titration, and quality...

  40. CRISPR Plasmids and Resources

    Type
    Collection
    ...CRISPR? Experts cover CRISPR topics on Addgene's blog. Addgene's Viral Service : Request ready-to-use viral...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...technologies are continously developing and expanding. Addgene works with leading scientists in the field to ensure...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety...zebrafish, C. elegans, parasites, and Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background...

  41. Plant Plasmids and Resources

    Type
    Collection
    ...- deposit plant plasmids with Addgene! CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...Promoter PI Return to top Other Resources Addgene Blog Posts Addgene Blog: Plant Biology RUBY-Red Siliques...Addgene's plant resources: Find plasmids for use in plants. Plasmid...Fluorescent Proteins Other Resources Background Addgene’s repository contains many plasmid tools that were...other species. In addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections... and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids containing promoters...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  42. TALEN Guide

    Type
    Collection
    ... TALENs TALEN Guide Addgene's Guide to TALEN Technologies You may also like....Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter Overview of TALEN Technology...these plasmids, which are available as a kit from Addgene , custom arrays consisting of 12-31 repeats can...and has already become the best-selling kit in Addgene’s history. Dr. Keith Joung’s lab at Massachusetts...Hospital also recently released a TALEN kit through Addgene. This kit, first described in Nature Biotechnology...at the Broad Institute, will be available from Addgene before the end of the year. Dr. Zhang ‘s lab published...could become a key tool in your plasmid toolbox. Addgene has rapidly become an important resource for this...

  43. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...Addgene AAV transfer plasmids...Available as a Viral Vector Packaging Service at Addgene ID Plasmid Capsid Description PI 112862 pAAV2/1...plasmid, expressing Rep2 and Cap2 Melina Fan , Addgene Research Program 232922 pAAV2/5 AAV5 AAV packaging...plasmid, expressing Rep2 and Cap5 Melina Fan , Addgene Research Program 240485 pAAV2/6 v1 AAV6 AAV packaging...plasmid, expressing Rep2 and Cap6 Meghan Rego , Addgene Research Program 112863 pAAV2/7 AAV7 AAV packaging...plasmid, expressing Rep2 and Cap11 Meghan Rego , Addgene Research Program 112866 pAAV2/rh10 AAVrh10 AAV ...helper plasmids (which may not be available at Addgene) may be required to produce infectious AAV particles...

  44. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...assistance at help@addgene.org . Don’t See What You’re Looking For? At this time, Addgene has made a limited...Grade DNA Service Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over ...start cloning upon arrival of their plasmid from Addgene – reducing the time to experiments by removing ...resources or scientific support? Please see our Addgene Protocols for resources you may find helpful. Our... Please note that this does not guarantee that Addgene will make cloning grade DNA preps of this plasmid...

  45. KLF Research Plasmids

    Type
    Collection
    ...benefits of depositing plasmids with Addgene or email deposit@addgene.org to get started. Genes KLF gene pages...Browse Addgene's collection of KLF plasmids and learn more about the scientists who have created and ...to the establishment of this shared resource on Addgene. If you have created plasmids containing KLF, we... KLF1 KLF2 KLF4 KLF5 KLF6 KLF Plasmids Browse Addgene's collection of KLF plasmids. ID Plasmid Description...

  46. Caltech Systemic Capsids

    Type
    Collection
    ...Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana ...As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use viral preparations...depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids . To review performance...data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository...produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and specific ... produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter...produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847) . Browse Available PHP.V1 AAV ID Name ...

  47. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...Industry Additional Addgene Resources Blog: Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids... viral proteins in several expression systems. Addgene partnered with Ginkgo to make these plasmids available...expressed or functionally validated by Ginkgo or Addgene. Plasmids Use the search bar or sort buttons to...

  48. Fluorescent Protein Guide

    Type
    Collection
    ...100 empty backbones. Additional Resources at Addgene Addgene Blog: Fluorescent Proteins Luciferase Plasmid... to find the plasmids that are available from Addgene's depositing scientists. Plasmid... Fluorescent Proteins: Plasmids and Resources Addgene's plasmid repository contains thousands of fluorescent...

  49. Synthetic Biology - Overview

    Type
    Collection
    ...about synthetic biology resources available at Addgene. Browse featured collections or depositing labs...engineering of biological systems for novel functions. Addgene is working with leading synthetic biologists around...facilitate the sharing of parts and information. Addgene can help you to share your synthetic biology plasmids...signaling and environmental sensing. Strains Browse Addgene's collection of engineered bacterial strains, including...Resource Allocator and Orthogonal Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  50. Worm Expression Resources

    Type
    Collection
    ...CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR...highlight some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools...of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description Gene...Biology Synthetic biology constructs - Browse Addgene's collection of synthetic biology plasmids for use... in C. elegans . Return to top Other Resources Addgene Blog Posts An “elegans” Approach to Better CRISPR...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
Showing: 1 - 50 of 1092 results