We narrowed to 412 results for: his

...discuss the various positions he has held in his career, his favorite company tradition, and Addgene's role...Development Manager, discusses his transition from the Scientist Team to Business Team, his hobbies, and advice ...

... 2, Corning 3269, 1272 cm 2 Heat-inactivated FBS (HI-FBS) Pro-Tip Different brands and lots of FBS can... sorbitol (optional) : DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, we have found ... M sorbitol, add 57 mL of 1.46 M sorbitol, 7.5 mL HI-FBS, and 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium...sterile 250 mL bottle. Aliquot 774 mL of DMEM + 2% HI-FBS into a sterile 1 L bottle. Add each plasmid DNA...solution to the bottle containing 774 mL of DMEM + 2% HI-FBS. Mix well. Take the CS5 out of the incubator...

...Trono 14666 pQE9-His-p97(wt) VCP His T5 ALS Graham Warren 14667 pQE9-His-p97(QQ) VCP His T5 ALS Graham Warren...17229 pQE9-His-p97deltaD2 VCP His T5 ALS Hemmo Meyer 17230 pQE9-His-p97deltaD2(K251A) VCP His T5 ALS Hemmo...0005 VCP His T7 ALS Axel Brunger 12339 pBD-0071 VCP His T7 ALS Axel Brunger 12344 pBD-0010 VCP His T7 ALS...0016 VCP His T7 ALS Axel Brunger 12346 pBD-0013 VCP His T7 ALS Axel Brunger 12347 pBD-0006 VCP His T7 ALS...0009 VCP His T7 ALS Axel Brunger 12349 pBD-0070 VCP His T7 ALS Axel Brunger 12350 pBD-0069 VCP His T7 ALS...0008 VCP His T7 ALS Axel Brunger 12352 pBD-0007 VCP His T7 ALS Axel Brunger 12353 pBD-0018 VCP His T7 ALS...0020 VCP His T7 ALS Axel Brunger 12355 pBD-0022 VCP His T7 ALS Axel Brunger 12356 pBD-0024 VCP His T7 ALS...

... CRISPR CRISPR History CRISPR History and Development for Genome Engineering ...

...Drosophila transgenesis His Epitope tag pEZYmyc-His - C-terminal Myc-His tag for mammalian expression...bacteria with N-terminal His-TrxA-TEV tag NusA Protein purification pNIC-HIS6-NUSA-TEV-GG - Cloning of...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac LIC vectors and pFastBac.... coli as TEV cleavable N-terminal His and NusA fusions Return to top Function Besides functions such ... with cap-dependent 3Myc-EYFP-HA-His6 and IRES-dependent ECFP-HA-His6 translation for mammalian expression... mammalian expression pET His6 GST TEV - N-terminal His6-GST-TEV for bacterial ...

...Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian Expression...dimerization pCEP4YPet-MAMM - Mammalian Expression pYPet-His - Bacterial Expression YPet-N1 - Mammalian Expression..._miRFP670 - Mammalian Lentiviral Expression pBAD/His-miRFP670 - Bacterial Expression iRFP670 643 670 13...pAAV-hSYN-miRFP670nano3-WPRE-hGHpA - Mammalian AAV Expression pBAD/His-miRFP670nano3 - Bacterial Expression miRFP680 661...Monomer pmiRFP703-N1 - Mammalian Expression pBAD/His-miRFP703 - Bacterial Expression iRFP702 673 702 7.4...Monomer pmiRFP709-N1 - Mammalian Expression pBAD/His-miRFP709 - Bacterial Expression mIFP 683 704 7 3.5....5 2.8 h Dimer piRFP - Mammalian Expression pBAD/His-B-iRFP - Bacterial Expression miRFP713 690 713 7 ...

...and cloned into an IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus...inexpensive to assay, and easy to purify using histidine-tagged constructs and routine protein and molecular...map, a TEV cleavage site is present between the 6xHis-tag and the coding region of MDH. For detailed clone...

...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Eric Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2...F391W-WPRE-SV40 Syn CaMPARI2-F391W his Constitutive 1 Eric Schreiter 101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40...-SV40 Syn CaMPARI2-L398T his Constitutive 1 Eric Schreiter Calcium Sensor: jRGECO1 100852 pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40...pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Syn jGCaMP8s none Cre-dependent 9 Mark Histed 176749 AAV-Syn-H2B-jGCaMP8s-WPRE Syn jGCaMP8s none...

...Benefits of HIS tags In addition to providing a way to purify a protein of interest, polyhisitidine tags can...protein of interest is fused to polyhistidine, then they can use an anti-HIS antibody, like this one developed...purification based on the polyhistidine tag. What is the polyhistidine tag? The polyhistidine tag is a string of....      Fig 2:  A, The polyhistidine tag is a string of histidine amino acids on the N- or C- terminus...residues Deciding how many histidine residues to use is a bit easier. The more histidine residues that are present... low level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended... of 6 or more histidine residues expressed in frame on the N- or C-terminus of a recombinant protein of...

...antibodies targeting V5, Protein C, DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please visit our Tags...high-confidence targeting of proteins that have historically been difficult to identify and interrogate with...

... lead his team as a scientists and a friend. George Church runs his lab fairly similarly to his advisor...the majority of his time in the lab.  As time went on and he was spending more time in his office, he gradually... always “off flying his plane to exotic places while occasionally emailing us his worldly advice”, the...package that included a postdoc and a PhD student in his first year. “It was very important to me to be with...adviser did? Tom Ellis merges the influence from his two PhD advisers who were “chalk-and-cheese”. While...

...contributed much of his knowledge to the CRISPR resources at Addgene. His background in history and specifically...spent most of his research career working on miRNAs and RNAi. He first heard about RNAi at his MIT interview... to the interview here! Doench majored in history during his undergraduate work at Hamilton College. When...that that’s the thing for you.” After recognizing his love for science and later realizing that he wanted...After a few rotations, Doench decided to complete his PhD work in Sharp’s lab.  Doench says, “what I loved...process. This early research is still relevant to his work at the Broad Institute today, where he helps... questions. Starting at the Broad Institute For his post-doc, Doench worked on using RNAi screens to ...

...be found on his website http://www.microworldsphotography.com/. Another artist who spent his life blurring...2008. His work exposes and explores the hidden structures within flowers with the use of x-rays. His haunting... non-academic people.” Daniel went on to discuss his work: “Macro photography has been my passion for ...Richards, a teacher of dental radiography, had focused his x-ray machine not on teeth, but on blossoms, thus...unique samples, such as those collected by Darwin on his voyage. Previous destructive techniques were incompatible...

... life. His lab has long hypothesized that life started with RNA and a simple cell membrane. His recent...depositor) Craig Mello. Taking his groundbreaking work in RNAi one step further, his lab is now studying how ...Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system. His lab is also exploring...presented by Todd Lowe from UCSC. Dr. Lowe talked about his work on the 500+ human tRNA genes and, until now,...role in prokaryotic immunity to viral invasion.  His lab is studying P. furiosus, a hyperthermophile that...resistant bacteria. See the picture to the left for his list of the bacterial species that “ESKAPE” antibiotic... structural studies on the ribosome performed in his lab. RNA is a structural and functional component...

...he hopes others will find his tools useful, including his zinc sensor and his genetically encoded magnesium...on the move, such as T lymphocytes. Griesbeck and his colleagues began by isolating a calcium-binding protein...Ratiometric imaging for beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila...Griesbeck also points out that the approach he and his colleagues took to optimize sensitivities can now...other published FRET-based sensors. For example, his team is now applying it to improve upon an indicator...than calcium, requiring greater sensitivity. He and his colleagues came up with a solution: they devised ...

...pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Biosensor Eric Schreiter AV-1-PV4704 101064-AAV1 pAAV_hsyn_NES-his-CaMPARI2... Schreiter AV-1-PV4705 101061-AAV1 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 Biosensor Eric Schreiter...

...Fluorescent Protein Application PI 14030 14031 pCyPet-His pYPet-His CyPet (donor) YPet (acceptor) FRET Patrick Daugherty...sites, and signal peptides include: Epitope tags: 6xHis, Flag, Strep II, c-Myc, HA, V5, GST Solubility tags...Venus) Escherichia coli Wolf Frommer 20336 pRsetB-his7-Perceval ATP:ADP ratio Fluorescence (GFP) Escherichia...

...covered! His His tags, often called polyhistidine tags, typically include six consecutive His residues...more). Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper, etc.), allowing His fusion...step metal affinity chromatography. Purification of His fusion proteins from E. coli is generally more straightforward...an antibody for that!   Fig. 1 - Diagram of His-tagged protein purification work flow by nickel column...Protein tags Antibodies 101: Epitope tags To Each HIS Own ...due to the presence of more naturally occurring histidine-rich proteins in mammals. Mammalian purification...

...same kinds of opportunities for his students. But he also found that his teaching responsibilities left...limited time for one-on-one mentoring. His solution? Bringing his research into the classroom. I had the...to hear about how he developed his program. Initially, Provost made his labs completely open-ended, allowing...San Diego), decided to take a different approach. His own most impactful learning experiences happened ... opportunity to talk with Provost recently about his development of what is now commonly termed a Course-based...encouraging them to engage in the problem-solving process. His previous experiences as a jazz musician, serviceman...serviceman in the U.S. Army, and hockey coach all shaped his teaching style, with the former offering excellent...

...21822235 . See the Q&A with Dr. Adam Bogdanove and his thoughts on the TALe technology here ....already become the best-selling kit in Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General...

...youngster. His parents, Kristi and Rico, had to keep him from running too much as any jarring of his body could...further impairs the patient. When I first met AJ and his family, he did not seem much different than my second... able to run around and play like the other kids his age. A potential FOP cure But, there is HOPE. FOP...working at Sanofi Pharmaceuticals in Cambridge, MA. His work experience is in both vaccine subunit design... along with pre-clinical analytical development. His areas of expertise include early stage drug discovery...

...was also a trained biologist. This is apparent in his work The Island of Dr. Moreau (1896) where he explores...attributed to SciFi writer Jack Williamson who used it in his 1951 novel Dragon’s Island. In 1976, the world’s ...wrong. The plot of the movie involves The Rock and his super giant albino gorilla fighting off a monstrous...more blog posts about CRISPR Learn more about the history of CRISPR Read about other genome editing methods...

...up to be one of the best of the best students in his home country. He was selected as one of 600 scholars...of science in Horticulutre Biotechnology, he did his PhD in Plant Breeding, Genetics, and Biotechnology...Labs, for lab equipment that will help accelerate his research. Listen to learn more about Dr. Kwapata’...Kingdom's research 28:08-END : Kingdom's Thoughts on his time in Boston and Outro We'd like to thank Dr. ...

...about his clinical trial patients with advanced macular degeneration; Laurenz Studer discussed his work...Shinya Yamanaka celebrate the tenth anniversary of his landmark Cell paper showing that it was possible ...been looking forward to seeing how the sharing of his OSKM factors have affected the stem cell community...as "life changing". Dr. Yamanaka himself, during his keynote address, appeared almost incredulous that... citing the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after...

...State University has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive... all those collaborations over the years, he and his colleagues built a Molecular Expressions collection...Davidson told the Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer that has...has now prompted him to deposit the tools he and his team created with the research community at large,...to promote the great work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael...

...received his Bachelor of Science degree in Computer Engineering from Tufts University and his Master of...co-founder of Addgene and received his PhD in Computer Science from MIT. His research focused on computer systems...

...current mechanistic understanding of GFP’s function. His lab described the protein’s structure and also discovered...local pet shop aquariums in Moscow where he lived! His hunch paid off, and he was able to report new fluorophores... Katushka/mKate from Entacmaea quadricolor. With his discoveries, another part of the fluorescent protein...

...Rett, who tried to raise awareness and published his observations in a German medical journal. Decades...to Rett Syndrome, this blog post describes the history of Rett Syndrome research, the role of the MECP2...

...bacteriophages. He decided to quit his "safety net" at the VC firm and devoted 100% of his energy and effort into ...co-founder of a biotech startup, Mike explained, his career transitioned away from the bench and became...VP. Mike was fortunate enough to be able to spend his last 6 months in graduate school focusing on concept...self-sustainable. Within a year from this decision, Mike and his co-founders raised $6 million. The biggest challenge...money, the team grew, and Mike stepped down from his CEO position and became first COO, then moved on ...

...Packer - science writer  When Hans was finishing up his PhD in Molecular Neuroscience at the University of... like a lot of newly minted PhD’s: not sure what his next step should be. He debated taking a position... on the job. It keeps it interesting.” And while his official title may be science writer, writing is ...is just the one of the skills Hans uses for his job. “I am really more of a multimedia science communicator...started working on them shortly after joining IDT when his boss was looking for someone to take over hosting...

...for any extrachromsomal hereditary determinant”. His proposal, however, was basically ignored. A separate...Learn more about the history of plasmids and cloning: CSHL Meeting: Plasmids: History & Biology The Joshua... history of plasmids...

...sharing. Listen to learn more about Darrell, his research, and his focus on open science. Listen to the interview...Darrell strives to promote open source biology in his own lab, at the Center for Regenerative Medicine,...Center for Regenerative Medicine. Darrell describes his career goals and what drives him as a praticing physician...

...  Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has ...Snapp, who is always looking for ways to improve his eBook, adds: "If you have any questions, comments...email me at [email protected]." You can find his original blog post below! --Ed.  Eight years ago...formula to guarantee a reader the faculty job of his/her dreams. There are too many moving parts in the...luck with your search!   Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology from...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...Research Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory...

...further with his new company, Science with Tom. In this podcast, we learn more about Tom and pick his brain ...and communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com.   Special...Rosalind Franklin vs. Watson & Crick - Science History Rap Battle A great example of science story telling...and their importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed...

...affinity tag is the polyhistidine (His) tag. Small and unlikely to affect function, His-tagged proteins can...using a Ni2+ column. Like other affinity tags, a His tag can be fused to either the N- or C-terminus of...for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification Very small ...which they can be added to a purification scheme, His tags are frequently combined with other tags for ...Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers, Ph.D., for her tips and guidance... signaling peptide (Bio), flanked by hexahistidine motifs (6xHis) MBP Large Protein  40 Solubility and...the tag size quickly – modifying the length a polyhistidine tract does not greatly alter the size of the...

...number of institutions in Ghana and most recently got his PhD in cell and molecular biology at the University...Novartis. Listen to learn how Kwabena plans to use his research experiences as well as plasmids from Addgene...50 : Why Kwabena chose to go back to Ghana after his PhD and the need to de-mystify molecular biology....

... work in DNA repair informed his decision to work with CRISPR during his postdoc 12:01 - 13:21 : The Wyss...Work with Cas9 Activators Michael Koeris Explains His Path from Grad Student to Entrepeneur Find Resources...

...As MIT's Ed Boyden explains it, his goal is "to understand the brain at a level of abstraction that enables... fix it when things go awry. To reach that goal, his group develops tools to tinker with brain circuits... article published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive ...optogenetics advance, the big questions that motivate his interest in neuroengineering, and some of the optogenetic...who may not be experts, can you give me a brief history of optogenetics? How long has this idea been around...

...optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian...optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian...Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian ...interactions in living cells using a microscope. Historically, molecular interactions were detected by indirect...

...which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have a FRET...used for protein purification: the NTA binds to the His tag on your protein and the coupled Cy fluorophore... Tc: CCPGCC 50 40-72 Intact Cells Cy3NTAc Poly His  44 35-63 Plasma membrane protein, permeabilized ... Tc: CCPGCC 48 38-70 Intact Cells Cy3NTAc Poly His  63 50-90 Plasma membrane protein, permeabilized ... Tc: CCPGCC 54 42-78 Intact Cells Cy3NTAc Poly His  65 52-94 Plasma membrane protein, permeabilized ...efficiencies between 80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby...department at Brigham and Women’s Hospital in Boston, MA. His interests include developing novel fluorescent tools...

...cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family spend family...Ellis highlights the importance of acknowledging his teams successes. “When PhD students graduate there...

...instead started a company around his own research. He was clear on his science, and found a solution. However...manageable laboratory environments. His business skills enhance his passion for science. Follow him on ...to provide the resources required for him to meet his goals. Therefore, he turned from academia altogether...

... Children’s Hospital and Harvard Medical School. His lab studies the molecular genetics of blood cell ...cell development and disease. Learn more at his webpage, bauerlab.org.     Matthew Canver is an MD/PhD student...laboratories of Stuart Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania...these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1 cleavage...

...problems resulting from past practices and mores.  His list is dire:  The current system is unsustainable... be in academia to make change.  He talked about his own unique way of funding basic research at NEB and... of the Novartis industry postdoctoral program.  His strongest comments were about the astounding lack...

...Genome Institute of Singapore who recently started his own lab. We discuss some of the joys and difficulties...Investigator at the Genome Institute of Singapore. His lab focuses on producing biotechnologies in the areas...

... Embracing Serendipity Nicolas Mathis, who shared information on his open software that predicts efficiencies.... Illuminating Choices Paul Heisig, who shared his flow cytometry expertise in three posts. An Introduction...

...given over the course of the day. His talk, which focused on the history of genome engineering from ZFNs...mentioned specifically in Dr. Zhang's introduction. His willingness to share reagents so freely with the ...analog versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed... of the book on my way to the meeting, and its history of code making and code breaking is wonderfully...

...Biol Chem. 2006) Express human ATM with Flag and His tags. (Canman et al. Science. 1998) See More CRISPR...95%) of ALS are sporadic, having no prior family history. A small percentage (5–10%) are familial ALS cases...

...about his own biases--many of which will be shared by fellow scientists--but that doesn’t make his introduction...case studies are “old,” they lead Zimmer to one of his most critical observations: “Today the most successful...if Zimmer was able to rewrite the last chapter of his book today--the day you are reading this review--...