... pAAV SYN1 HA-hM4D(Gi) hM4D(Gi) - Inhibition HA fusion none 2, 5 Richie 121539 pAAV SYN1 HA-hM3D(Gq) hM3D... Roth 50454 pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine ... Roth 50455 pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine ... hM3D(Gq) - Activation HA fusion none 2, 5 Richie 50467 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi...* Fishell 50472 pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine ...CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent...1, 2, 5, 8, 9, rg*, PHPeB Roth 65417 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - ...

...pCINeo-hFX-HA FXN HA, AU1 CMV Friedreich ataxia Michael Ristow 14977 pCINeo-hFX(I154F)-HA FXN HA, AU1 CMV...pBlue-hFX-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14988 pBlue-hFX(I154F)-HA FXN HA, AU1 T7 Friedreich...pBlue-hFX(T133A)-HA FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 14990 pBlue-hFX(T133E)-HA FXN HA, AU1 T7 Friedreich...-hFX-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 14992 pET28(a)-hFX(I154F)-HA FXN HA, HIS,...AA88)-HA FXN HA, HIS, AU1 T7 Friedreich ataxia Michael Ristow 15403 pBlue-hFX(I154F)(AA88)-HA FXN HA, HIS...pBlue-hFX-HA(AscI) FXN HA, AU1 T7 Friedreich ataxia Michael Ristow 15405 pBlue-hFX-HA(NcoI/EcoRI) FXN HA, AU1...Camilli 22563 Flag-HA-UCHL1 UCHL1 HA, FLAG Parkinson's Wade Harper 22594 Flag-HA-OTUD4 OTUD4 HA, FLAG CMV Gordon...

...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available... it with your organization. If your organization has not ordered from Addgene in the past, we will need...regarding your account verification. Once your account has been verified, you will be able to place your order...our Help Center . The MTA Process: Once your order has been placed on our website, Addgene will email you...behalf of the recipient institution. Once the MTA has been signed, it can be submitted to Addgene through...

...fairly high specificity. Fifteen years of research has shown that when fused to a nuclease or activation...limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and interactions between...with Adam Bogdanove ), progress on TALe research has been swift. The incentives are high. Since ZF targets... TALEN kit came online in the summer of 2011 and has already become the best-selling kit in Addgene’s ...co-founder of the Zinc Finger Consortium with Dr. Voytas, has considerable experience in the DNA targeting field...become a key tool in your plasmid toolbox. Addgene has rapidly become an important resource for this new...

...wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target effects...seminal CRISPR article from Dr. Feng Zhang's Lab, has assembled a list of FAQs about using the lab's CRISPR...Dr. Feng Zhang's Lab ( Science , February 2013 ), has assembled a list of FAQs about using the lab's CRISPR... a new system, developed by the Zhang lab, which has comparable efficiency to the optimized chimeric design...of references. For genome editing, the Zhang lab has published this Nature Protocol article . Should I...sequence is present in both alleles. The Zhang lab has found that if a CRISPR is used to engineer a locus...relative to where the homology ends? The Zhang lab has always used homology arms that are less than 15bp...

...Backbones Flag Epitope tag pcDNA3 Flag HA - N-terminal Flag-HA for mammalian expression pNIC-CTHF...Backbones Mammalian CMV, SV40, EF1a, CAG pSG5L Flag HA - Transient expression pWPXL - Lentiviral... your protein to an epitope tag, such as Flag or HA, will allow you to easily identify your protein...variety of systems, including Xenopus HA Epitope Tag pKH3 - N or C-terminal ...expression pHAHA - N-terminal double HA tag for mammalian expression pCS2HA - N or C-terminal..., including Xenopus pAG423GPD-ccdB-HA - C-terminal 3xHA tag for yeast expression...dividing and nondividing cells Addgene has an extensive collection of plasmids for ...

...Design Tools CRISPR Blog Posts The CRISPR system has been adapted to allow tagging of proteins expressed...Yamamoto PITCh Tagging System The Takashi Yamamoto lab has deposited the PITCh system plasmids for CRISPR-based...Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based ...Drosophila Cell Tagging System The Förstemann lab has developed a CRISPR tagging technique for use in Drosophila...et al. Nucleic Acids Res. 2014 The Förstemann lab has provided detailed protocols for N- or C-terminal .... elegans Tagging System Geraldine Seydoux's lab has developed a systematic and scalable method to create... Collection The Allen Institute for Cell Science has produced the first publicly available collection ...

... visualize targeted genomic loci. CRISPR imaging has numerous advantages over other imaging techniques...dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking of repetitive and ...visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity. A...

...sequence has been validated by next generation sequencing. The DNA concentration and 260/280 ratio has been...See What You’re Looking For? At this time, Addgene has made a limited number of plasmids available as cloning...

...in both tetracycline on and off systems. Dox also has good tissue distribution, low toxicity, a known half-life...expression of shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain 41393 pCW57.1...that is silent in absence of binding tTA or rTA; has high copy E. coli origin None Either Ralser 64238...60495 pSBtet-GP Sleeping Beauty transposon system; has luciferase in cloning site; see article for additional...to make cell lines Tet-On Advanced; Plasmid 26730 has hygro resistance 3rd generation rtTA On Campeau 25434...linked via IRES to EGFP-Blasticidin fusion; pMA2641 has rtTA driven by retroviral LTR promoters ; see article...

...plasmids. Ginkgo Bioworks Plasmid Collection - Addgene has partnered with Ginkgo Bioworks to distribute SARS-CoV...DETECTR . Feng Zhang's lab at the Broad Institute has utilized the natural RNAse activity of the Cas13 ...UnLOCKING (SHERLOCK and SHERLOCKv2). The Broad Institute has published information and protocols for utilizing...bioRxiv 2020.04.20.052159 . Jennifer Doudna's lab has utilized Cas12a’s non-specific ssDNA degradation ...CRISPR Trans Reporter (DETECTR). Mammoth Biosciences has published information and protocols for utilizing... out this Suggest a Plasmid form. If the plasmid has been described previously we will reach out to the...

...of Michael Davidson from Florida State University has contributed their comprehensive collection of ORFs... data found below. In addition, the Davidson lab has contributed the corresponding empty backbone with...

... proteins in several expression systems. Addgene has partnered with Ginkgo to make these plasmids available...yeast, or mammalian) Tags (e.g. 6xHis, Flag, StrepII, HA, Myc, SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or...

...The Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins...Plasmids Protocols The Allen Institute for Cell Science has produced the first publicly available collection .... Protocols The Allen Institute for Cell Science has several protocol videos available that may be helpful...

...Voytas Lab plasmids CRISPR plasmids The Joung Lab has developed three platforms for engineering TAL effector...effector repeat arrays produced on this framework has yielded TALENs which function efficiently in: C. ...

... dynamic range, and resistance to photobleaching has made luciferase a common choice in assays ranging...second-generation version of Firefly luciferase that has been codon optimized for expression in mammalian ... Firefly luciferase, Click-beetle red luciferase has also been codon optimized ( CBR2opt ) for mammalian...mammalian expression systems. Each type of luciferase has advantages and disadvantages depending on the application...before or after luciferase . In addition, Addgene has many luciferase expression constructs available that...expression renilla luciferase mutant Rluc8, which has increased stability and light output Sanjiv Sam Gambhir...

... for Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish... The Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish...

...tropical freshwater fish in the minnow family. It has long been established as a powerful vertebrate model...genes having an ortholog in zebrafish, this species has more recently emerged as a model system for human... amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible...

...respectfully). This next generation architecture has been shown to increase mutation induction when using...after the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid 47389 also contains the VP64 domain...double-selection of transfected cells, the system has successfully been employed for deletion mutagenesis...

...New England Biolabs has deposited plasmids at Addgene to generate SNAP, CLIP, ACP or MCP tagged plasmids...PDF, 46 KB) Please note: New England Biolabs (NEB) has discontinued the ACP-tag/MCP-tag product line from...