...SponGee: A Genetic Tool for Subcellular and Cell-Specific cGMP Manipulation. Cell Rep. 2019 Jun 25;27(13):...Islands of Cells for multimodal recordings of cellular physiology. Nat Commun. 2020 Aug 4;11(1):3881. Adam... Tantama ATP QUEEN ATP sensors for live-cell imaging Live cell imaging of metabolic heterogeneity by quantitative...Islands of Cells for multimodal recordings of cellular physiology. Nat Commun. 2020 Aug 4;11(1):3881 Adam...for Imaging Insulin Secretion in Single beta Cells. Cell Chem Biol. 2017 Apr 20;24(4):525-531.e4. Dmytro...accessibility. Cell. 2022 Sep 1;185(18):3390-3407.e18. David Clapham Return to Top Cell Physiology Target...

...receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor Gadd45 ...-phase expressed 1; also known as GTSE1 BAI-1 Brain-specific angiogenesis inhibitor 1 Bax BCL2-associated...inhibitor type 1), member 1 PERP TP53 apoptosis effector PIDD p53-induced death domain protein 1 PIGs Etoposide...p53 are in promoting cell cycle arrest, apoptosis, or senescence in damaged cells. The p53 name arose ... Name 14-3-3-σ Stratifin Apaf-1 Apoptotic peptidase activating factor 1 ATM ATM serine/threonine kinase...kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase 2 Cop-1 Ring finger and WD repeat domain 2...SESN1 SESN2 SESN3 Sestrins 1, 2, or 3 Siah Siah E3 ubiquitin protein ligase 1 TSC2 Tuberous sclerosis 2...

...Laplante M, Sabatini DM. Cell. 2012 Apr 13;149(2):274-93. doi: 10.1016/j.cell.2012.03.017. PubMed PMID... (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine/threonine...signals to determine the nutritional state of the cell and regulates the switch from anabolic to catabolic...growth factors, including insulin. mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact... promotes the conditions needed for uncontrolled cell growth and proliferation, including an abundant ...appears to signal upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids...threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40 Also known as AKT1S1...

...IGHDY1 IGHD1-1 immunoglobulin heavy diversity 1-1 IGHD11 IGHD1-14 immunoglobulin heavy diversity 1-14 (non-...IGHV6-1 immunoglobulin heavy variable 6-1 IGHV61, VH IGHV7-4-1 immunoglobulin heavy variable 7-4-1 IGHV7...foreign antigens to adaptive immune cells, such as T cells and B cells. About a week following the initial...presenting cells. B cells in secondary lymphoid organs recognize specific antigens through the B cell receptor...have been described. NK Cell Signaling Natural killer (NK) cells can kill cells that have been infected... within an infected cell membrane, triggering cell death. TCR Signaling The T cell receptor (TCR) engages...through effects on numerous immune cell types including T cells and B cells. It also impacts the development...

..., complex 1 SAV1 Salvador family WW domain containing protein 1 SCRIB Scribbled planar cell polarity protein... of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 PAK2 PAK3 PAK4 p21 protein (Cdc42/Rac)...TANK binding kinase 1 TFDP TFDP1 TFDP2 Transcription factor Dp TIAM TIAM1 TIAM2 T-cell lymphoma invasion... dependent protein kinase 1 PEBP1 Phosphatidylethanolamine binding protein 1 PIK3 Catalytic Subunits PIK3CA...isomerase, NIMA-interacting 1 PLXNB1 Plexin B1 PPP1CA Protein phosphatase 1 catalytic subunit alpha PREX2...family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase 1 RHEB Ras homolog enriched in ...guanine nucleotide exchange factor 1 YAP1 Yes associated protein 1 Return to top Resources RAS Pathway...

...for pMKO.1 puro; Contains shRNA against GFP William Hahn 10676 pMKO.1 GFP Derivative of pMKO.1 puro with...functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Rubinson...pLKO.1 ‐ TRC control Negative control vector containing non-hairpin insert David Root 1864 pLKO.1 ‐ scrambled...Description PI 8452 pMKO.1 puro Retroviral vector for shRNA expression Bob Weinberg 10675 pMKO.1 puro GFP shRNA ... an RNA-mediated gene silencing mechanism. As a tool in mammalian cell systems, silencing is achieved ...expression; Designed for inducing RNAi in a wide range of cell types, tissues and organisms Luk Parijs 11619 pLB...Hacohen N, Hahn WC, Lander ES, Sabatini DM, Root DE. Cell. 2006. 124:1283-1298. PubMed . A versatile tool ...

...with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell Stem Cell . 17 (2), 221–232. PMID: ...in the same cell. This is called multiplexing , and increases the likelihood that any cell containing ...can increase efficiency. Synchronizing cells or modulating the cell cycle can help as HDR takes place during...expressing stable cell line. After applying your screening conditions to your transduced cell population, you...tracking of RNA in live cells, including orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview...promiscuity is unique to bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the ...genomic loci in living human cells by an optimized CRISPR/CAS system. Cell . 155 (7), 1479–1491. PMID: ...

...genomic loci in living human cells by an optimized CRISPR/Cas system. Cell . 155(7):1479-91. PMID: 24360272... for Mapping Multiprotein Complexes in Human Cells. Cell Rep . 13(3):621-33. PMID: 26456817 Fu Y, Sander... cleaves it to destroy the invader ( Figure 1 ). Figure 1: An overview of the endogenous Type II bacterial...described CRISPR-based genome editing in mammalian cell culture. Five years later, PubMed lists more than...multiple loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR...genome edits or double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require a specific flanking...proteins can be used to image genomic loci in live cells. RNA targeting : Cas13 enzymes target RNA rather...

...genome editing in mammalian cells (Cong et al ., Science 2013, Ran et al ., Cell 2013, Ran et al ., Nature...Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6...Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):1380-9. doi: 10.1016/j.cell.2013.08.021. Epub 2013 Aug...Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi: 10.1016/j.cell.2014.09.014. Epub 2014 Sep...CRISPR/Cas system can be implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease...cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system can ...

...be repaired by the cell’s endogenous repair mechanisms. Cas3 belongs to the Class 1 family of CRISPR systems...individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com. Browse...bacteria and archaea. While abundant in nature, Class 1 systems are largely underutilized compared to their...

...Unpublished Joost Gribnau Human Cell Line Models Induced pluripotent stem cells (iPSCs) are a powerful system...opens in a new window) LUHMES cell line , an immortalized pre-neuronal cell line that can be differentiated... RSRT cell line collection (186.8 KB) . To order, please email [email protected] The following cell lines ...information Mouse and Cell Line Repositories (Link opens in a new window) Coriell Institute - cell line repository...approximately half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these...Plasmid Collection and Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources... treat Rett syndrome and restore functional MECP2 in cells. Search (Link opens in a new window) JAX Mice...

...preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...Bullock and Port 49411 pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3 BbsI Injection or in vitro transcription...O'Connor-Giles, Harrison, Wildonger 49408 pCFD1-dU6:1gRNA dU6:1 BbsI Injection or in vitro transcription Virmilion...

...190293 Anti-AMIGO-1 [L86/14] AMIGO-1 Mouse Mouse IgG2a 190294 Anti-AMIGO-1 [L86/2] AMIGO-1 Mouse Mouse IgG2a...ATPase 1 [L60/4R-2b] Copper ATPase 1 Human Mouse IgG2b 206648 Anti-AMIGO-1 [L86/33R-2b] AMIGO-1 Mouse ...channel [L6/60R-1] Slo1 K+ channel Mouse Mouse IgG1 206695 Anti-AMIGO-1 [L86/33R-1] AMIGO-1 Mouse Mouse IgG2a...Anti-Olig1 [N149/25R-1] Olig1 Mouse Mouse IgG1 206702 Anti-Neurexin-1-Beta [N170A/1R-1] Neurexin-1-Beta Human Mouse...222160 Beclin-1 [N248A/15R] Beclin-1 Mouse Mouse IgG2a 222161 Beclin-1 [N248A/22R] Beclin-1 Mouse Mouse ...Mouse Mouse 190534 Neurexin-1-Beta scFv [N170A/1] N170A/1 scFv Neurexin-1-Beta Human Mouse 190535 PSD-...Mouse 206755 IP3 receptor, type 1 scFv [L24/1] L24/1 scFv IP3 receptor, type 1 Rat Mouse 206756 VSP scFv [...

...preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...researchers use ssDNA or dsDNA repair templates with 1. homology to the DNA flanking the DSB and 2. a specific...

...enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs from deleting both...gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids 159654... make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence.... Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express... pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene #110821 ) VPR ( Addgene #63798) Plasmids...

...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single... find a particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description...Viral Pseudotyping SARS-CoV-2 Pseudotyped Virus Mechanisms of SARS-CoV-2 infection remain poorly understood...This approach allows researchers to safely study mechanisms of infection and to determine methods of neutralizing...

...others): caveolin 1, also known as CAV 1. Usually, caveolin 1 promotes the cell life cycle progression and ...and spread of cancer cells. Besides these stromal cell modifications, stromal cells are also constantly...how cancer cells influence microenvironments and vice versa, let’s take a look at cancer cell metabolism...inflammation, a noteworthy cell type in the tumor microenvironment is the immune cells. The contradiction is...injecting breast cancer cells into mice, exosomes expressed specific integrins (cell-adhesion proteins) targeting... be transferred to other cells, inducing epigenetic changes in targeted cells. In this case, exosomes ...Friendly” microenvironment cells Not all is bad news – we also have “friendly” cells in microenvironments! ...

...to activate programmed cell death or dormancy for phage-infected bacterial cells so as to limit the spread...preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of specific genomic regions...

...with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell Stem Cell . 17 (2), 221–232. PMID: ...in the same cell. This is called multiplexing , and increases the likelihood that any cell containing ...can increase efficiency. Synchronizing cells or modulating the cell cycle can help as HDR takes place during...expressing stable cell line. After applying your screening conditions to your transduced cell population, you...tracking of RNA in live cells, including orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview...promiscuity is unique to bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the ...genomic loci in living human cells by an optimized CRISPR/CAS system. Cell . 155 (7), 1479–1491. PMID: ...

...transfected cells. k. Incubate cells at 37°C, 5% CO 2 for 24 hours. Day 4: l. Harvest media from cells and transfer...concentration for your cell line before initiating your experiment. Day 1: a. Plate target cells in ten 6 cm plates...plates from 1-10 and add appropriate puromycin-containing media to cells. Days 3+: e. Examine cells each day...Selection Day 1: a. Plate target cells and incubate at 37°C, 5% CO 2 overnight. Day 2: b. Target cells should...for your target cells. The final concentration of puromycin should be from 1-10 μg/mL in 1 μg/mL increments...of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids...Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2 Annealing...