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We narrowed to 304 results for: rigi

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Showing: 1 - 20 of 304 results

  1. A Modular, Synthetic Origin of Replication

    Type
    Blog Post
    ...rebuilt the classic pMB1 origin of replication into a modular, programmable origin we call SynORI. Instead...clunky. To address this, we created a synthetic origin of replication (ORI) that allows us to modify the...unclear, but the result suggests that refactoring the origin may have compromised a stability feature. As a ...resources Resources on the Addgene blog Plasmids 101: Origin of Replication Plasmids 101: Plasmid Incompatibility...Resources on addgene.org Watch our video on the Origin of Replication Read our Plasmids 101 ebook Get ...

  2. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...any plasmid: the origin of replication/replicon. The replicon is comprised of the origin of replication ...making copies.     Types of origins of replication There are lots of origins of replication out there so...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic...known derivatives), pSC101, R6K, and 15A. Not all origins of replication are created equal. Some will produce...proper promoter and growth conditions. Choose your origin of replication wisely and avoid plasmid incompatibility...

  3. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...of interest, a medium copy RSF1030 origins or a low copy p15a origins should be used. Find further details...other plasmids containing high copy ColE1/PUC-like origins. Therefore, when choosing plasmids to express the...for 2014 Phosphoprotein Reagents (PDF, 691.3 KB) Original Kit (ca. 2011) described in: Park, H., Hohn, M...

  4. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...you have the right to use the original plasmid. Distribution of the original plasmid is important because...which is a sample of the purified DNA from the original plasmid that was used to make the virus. In order...

  5. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Information Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN...tools for using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove...

  6. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...in order to proceed with any adjustments to the original order. Turnaround Time Production of a Packaged...in order to proceed with any adjustments to the original order. Experimental Disclaimer Due to the novel...

  7. CRISPR Library Amplification

    Type
    Protocol
    ...libraries may require modifications dictated by the originating laboratory for optimal results. If you obtained...the elements required for bacterial propagation (origin of replication and antibiotic selection). This ...option is to perform a gel extraction of either the original sample or the amplified sample, followed by reamplification...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase reporter. These plasmids improve on the original STARR-seq by using the bacterial ORI as the core...which was a source of false positives with the original. Structurally destabilized firefly luciferases...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... Map of pLKO.1 containing an shRNA insert. The original pLKO.1-TRC cloning vector has a 1.9kb stuffer ...Self-inactivating long terminal repeat. f1 ori f1 bacterial origin of replication. Amp R Ampicillin resistance gene...plasmid in bacterial cells pUC ori pUC bacterial origin of replication. 5’LTR 5’ long terminal repeat. ...

  10. Antibody Guide

    Type
    Collection
    ... and can either be used while suspended in the original media or isolated and placed in a buffer. Antibody...antibodies can distinguish between different species of origin, isotypes, and isotype subclasses of a primary ...

  11. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...antibody-expressing plasmids containing the SV40 origin of replication use a HEK293 line stably expressing...antibody-expressing plasmids containing the Epstein-Barr virus origin of replication use a HEK293 line stable expressing...

  12. CRISPR Guide

    Type
    Collection
    ...Overview of the basic CRISPR mechanism. CRISPR was originally employed to knock out target genes in various..., be sure to check the library information and original publication to see if a library is suitable for... of your amplified library matches that of the original library. Next, you’ll need to generate lentivirus...

  15. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...990 KB) Plasmid and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2...

  16. Mammalian RNAi Tools

    Type
    Collection
    ...References Carthew, R. W., & Sontheimer, E. J. (2009). Origins and mechanisms of miRNAs and siRNAs . Cell, 136...

  17. Serotype Testing AAV

    Type
    Collection
    ...which is a sample of the purified DNA from the original plasmid that was used to make the virus. For more...

  18. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...prime editing. Created with BioRender.com. The original prime editing enzyme is named PE1 . PE1 and most...

  19. Worm Expression Resources

    Type
    Collection
    ...WormBook - A comprehensive, open-access collection of original, peer-reviewed chapters covering topics related...

  20. TALEN Guide

    Type
    Collection
    ...TALEN Kits CRISPR Plasmids Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter...
Showing: 1 - 20 of 304 results