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We narrowed to 1,052 results for: STI

Showing: 181 - 200 of 1052 results
  1. A Guide to Getting Started in Undergrad Research

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    Blog Post
    ...person. I like to ask questions and think about the best way to answer those questions. I’ve met graduate...about the science allows you to ask more interesting questions and your excitement will in turn inspire...lab? Ask your questions—especially if it’s scary. I’ve been afraid to ask “dumb questions” and to make ...dumb and very basic question was actually a great one. Further, even if a question is a very basic one...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational... sciences), people conduct research to answer questions about how the world works. As scientists, we probe...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...
  2. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...negotiate for a win-win decision An institute/department is investing in you, and is counting on your research... the institute time and energy that would typically be wasted going through some of the questions we mentioned...a junior investigator turned down a faculty position because none of the offering institutes were able...poor outcomes; not enough funds to get started, wasting funds on unnecessary materials, or even a loss ...specific aims. This is actually why a department/institute is interested in you. Your science will define...based on these findings. Most often we suggest estimating the time it will take to secure your first (R-level...greater than $5,000 but this can depend on the institution/funding source. Major equipment includes large...
  3. CRISPR Plasmids - RNA Editing

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    Collection
    ...Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should be added to this...
  4. Bringing Sustainable Practices to the Lab: Recycling

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    Blog Post
    ...we account for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled...mail. Recycle plastic packaging Packaging is a huge contributor to the increase in plastic manufacturing...services don’t offer plastic recycling, specialized companies can recycle plastic-- you just have to find.... This service does cost money, but your institute may still be interested as it offsets the cost of regular...recycling! With the popularity of single-use items, plastic is a substantial portion of lab waste. Despite ...label. To return the box, simply peel your address sticker off the box, remove the ice pack (it’s not recyclable...manufacturing, accounting for 40% of total plastic usage (Geyer et al., 2017). While most single-stream or city ...
  5. The Materials Science of Optogenetics Experiments

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    Blog Post
    ...thus individual laser stimulation is essential. In terms of the laser stimulation itself, the output of...battle when using optogentics to answer a research question. The flip side of the optogenetics coin is materials...the dorsal/ventral location of your region of stimulation (the z-axis bregma coordinate). Because the light...it enters tissue, placement above the site of stimulation guarantees that the majority of the opsins will...should be about 0.4-0.5mm above the site of your stimulation. The scatter effect is also an important consideration... polished to significant smoothness to prevent drastic loss of efficiency between patch cable and implant...during the entire protocol, otherwise optical stimulation will be erratic, opsin activation/inactivation...
  6. CRISPR Plasmids - Epigenetics

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    Collection
    ...Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should be added to this...
  7. Trading Spaces - Visiting Scientist Edition

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    Blog Post
    ... host lab or institute provides something that you can’t obtain at your current institute. A common reason...don’t have, or maybe both, a visiting scientist stint may be ideal for you. Here, we will chat about who...scientist, in academia, is someone who visits an institution to perform research there temporarily. The length...The researcher remains employed by their home institute, meaning the host university does not hire or ... pay them (typically). A visiting scientist is distinct from equipment sharing or a collaboration – it...requires re-location of the scientist to the new institute where their typical workdays will be carried out...back to your home lab. Alternatively, the host institute may have a piece of equipment that is custom-built...
  8. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post
    ...specific statistical test.  Preregistration makes clear the distinction between hypothesis testing work, ... and other questionable research practices (QRPs) can quickly lead us to find statistically significant...encouraged to present that work using hypothesis testing statistical tools that generate a p-value.  As an example...authors are able to make that clear distinction between hypothesis testing and generation, can be sure that...biomedical and psychological sciences were called into question after credible attempts to replicate major findings...instead of the methods. Unreported flexibility and questionable research practices In an attempt to present ...and hypothesis generation, in which data are investigated to look for unexpected trends or differences...
  9. MAPK Plasmids

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    Collection
    ...Wikipedia, with PyMOL. Author: Bubus Do you have suggestions for other plasmids that should be added to this...
  10. CRISPR Plasmids - Bacteria

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    Collection
    ...Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should be added to this...
  11. CRISPR Plasmids - Parasites

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    Collection
    ...Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should be added to this...
  12. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should be added to this...
  13. Fluorescent Protein Guide

    Type
    Collection
    ...Proteins Fluorescent Protein Plasmid Kits Allen Institute for Cell Science Plasmid Collection New England...
  14. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post
    ...process. This early research is still relevant to his work at the Broad Institute today, where he helps researchers... to answer their own research questions. Starting at the Broad Institute For his post-doc, Doench worked...towards the Broad. In order to ask interesting biological questions, you need a tremendous amount of technology...with scientists asking biological questions. Doench loves that he still gets to see data as it’s coming ...the Broad Institute here Read other blog posts about CRISPR Learn more about the Broad Institute and the...the Genetic Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve... School and then applied to work at the Broad Institute: “The model that the Broad was building that had...
  15. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...(BRET) were developed by the Johnson Lab . Characteristics of the pHlash reporter protein include: H+...large changes in binding affinity with light stimulation. Indeed oLID showed only a two-fold change in...50 fold change in binding affinity with light stimulation2. The Kuhlman lab created two improved Light ...specific genes via user-defined guide RNAs. Upon stimulation with blue light, the complex recruits a CIB1 ...pUCXKT, removing the stop codons during backbone digestion and providing the missing codons for functional...C-terminal tags. Additionally, by using an adapted destination vector with a heterologous promoter outside of...create an Entry vector containing a promoter for constitutive (CMV, SV40, SFFV) or inducible (CMV-TO) cDNA...
Showing: 181 - 200 of 1052 results