...Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including ...and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components....networks. Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing...

...nematodes, including C. elegans and C. briggsae. Other related miniMos plasmids can be found on the Jorgensen ...genetics, genomics and biology of C. elegans and related nematodes. WormBook - A comprehensive, open-access...original, peer-reviewed chapters covering topics related to the biology of C. elegans and other nematodes...

...sensor (MARIO) A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic ...through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2. bioRxiv...Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Proc Natl Acad...sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566. Yulong Li Serotonin...-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(...

...Like other transversion editors, this editor had relatively high indel rates ranging from 4.7% to 30% at ...was on its way! All base editors up to this point relied on deaminase enzymes, either to directly accomplish...

...Uses of biotin in molecular biology Biotin is a relatively small, water soluble molecule that does not interfere...of some other common applications and links to relevant papers and webpages are listed below: EMSA (Electrophoretic...

...technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move ...next-generation sequencing techniques do not typically rely on Sanger sequencing but instead use methods like...

...common disorders followed by a list of some of the relevant collections available at Addgene. Amyotrophic ...growing collection of general and neuroscience -related antibodies including: Anti-KChIP1 K+ channel [K55...diagnostics and treatments for Alzheimer’s disease and related disorders. The site curates information on useful...

...beginning or end of a sequencing reaction is often unreliable. Although your sequencing results may indicate... file, you will see that these base calls are unreliable. This is an example of a trace file from a portion...

... opens in a new window) Video Link Description Related Page Making LB Agar Plates Create plates to culture... opens in a new window) Video Link Description Related Page MTA Guide - Requests Your guide to the MTA...

...insert donor DNA at “landing sites” defined by relatively short sequences. Some have been paired with CRISPR...high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template to accurately... transduced cell population, you will look for relevant hits using NGS technology. For more detail on ...As this reporter is degraded by Cas12a, it will release a quantifiable signal that indicates the presence... J., & Van Der Oost, J. (2012). CRISPR immunity relies on the consecutive binding and degradation of negatively...

...20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-1-20297P 20297-AAV1 pAAV-EF1a-double ...20071P 20071-AAV9 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-9-20297P 20297-AAV9 pAAV-EF1a-double ...Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda AV-5-20949P 20949-AAV5 pAAV-double floxed-eNpHR-EYFP-WPRE-pA...

...Addgene's collection of synthetic biology plasmids related to Networks and Gene Regulation...Addgene's collection of synthetic biology plasmids related to Networks and Regulation. The collection includes...

... therapeutics, and vaccines against COVID-19 all rely on DNA encoding viral proteins as a first step for...articles, protocols, and other resource collections related to COVID-19 that may be of use to scientists. Addgene's...

...dimensional structures of proteins of medical relevance, and place them in the public domain without restriction...TEV cleavage, Bac-to-Bac Expression System, AmpR Related Resources SGC: http://www.thesgc.org/ SGC Toronto...

...support educators interested in integrating MDH-related research into their teaching laboratories. They... plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression, structure ...

...laboratory mouse. It’s also made targeted insertions relatively simple in C. elegans and bacteria. But CRISPRing...

...the intended edit must be in a 5 - 10 nt window relative to the PAM, and bystander edits are possible if...introduction of a specific mutation, one generally relies on homology directed repair (HDR) to incorporate...along with the single cell clone (although this is rarely done). When targeting a dsDNA break for HDR, the... has been shown that the FANTOM database, which relies on CAGE-seq to directly capture the mRNA cap, provides...

...long been extremely useful in the lab – they are relatively small and easy to work with, making them the ...the day, March 16, 2014, that I got my first preliminary results from a CRISPR genome editing experiment...

... when the genome editing frequency is low, the relative abundance of exon-skipped mRNA is high, likely...and large animal models with mutations in human-relevant exons 45-55, rather than in the traditional exon...

...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency...the reverse transcriptase. Many PE enzymes and related tools are available: PE2 - introduction of five...