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We narrowed to 1,064 results for: tra

Showing: 181 - 200 of 1064 results

  2. MAPK Plasmids

    Type
    Collection
    ...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...

  3. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...homology to the crRNA. This activity provides a stark contrast to Cas9 and Cas12, which require that each DNA...

  4. Fluorescent Protein Guide

    Type
    Collection
    ...Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent...

  8. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...

  9. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ... and 159655 are single guide expressing double stranded break constructs. Plasmids 171098 and 175570 allow...

  13. Control AAV Preps

    Type
    Collection
    ... Cre dependent 5 Tim Brown and HHMI-JRC Tool Translation Team 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry...

  14. General Transfection

    Type
    Protocol
    ...for transfecting mammalian cells using linear polyethylenimine. Transfections allow for transient expression...RT. Carefully transfer the transfection mix to your HEK293T cells. Add the transfection mix dropwise, ... viral production) Day 1: Transfect Cells Day 2 (am): 18 h post transfection - Remove media, replace with...protocol to transfect mammalian cells with your plasmid of interest. Protocols... Protocols General Transfection General...General Transfection You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service...adapted for different cell lines and different transfection reagents. Workflow Timeline Day 0: Seed HEK293T...

  15. Protocol - Bacterial Transformation

    Type
    Protocol
    ...Introduction Transformation is the process by which foreign DNA is introduced into a cell. Transformation of bacteria...modifications to create bacterial strains that can be more easily transformed and that will help to maintain...prepared for optimal transformation efficiencies upon thawing. For the highest transformation efficiency, we...time when carrying out transformations? If you are not concerned with transformation efficiency (such as ... 20-30 mins) I get very few if any transformants when transforming large plasmids (>10 kb) or BACs, what...Learn how to transform E. coli with your plasmid of interest. Protocols... Protocols Bacterial Transformation Bacterial...

  16. AAV ddPCR Titration

    Type
    Protocol
    ... Protocols ddPCR Titration of AAV Vectors ddPCR Titration of AAV Vectors You...for purified AAV titration. Protocols...You may also like... AAV Titration by qPCR AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular...samples. ITR Master Mix Volume 9X Volume Final Concentration 2X ddPCR Supermix for Probes, no dUTP 10 µL ...the DG8 gasket, making sure that it is secure. Transfer the cartridge holder to the droplet generator....while slowly and gently aspirating the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip... 98 10 2 1 Hold 4 ∞ 2 1 After PCR is complete, transfer the plate to the Droplet Reader. Open the QuantaSoft...

  17. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Colony Formation Titration Assay Lentivirus Fluorescence Titration Assay AAV Titration by qPCR Addgene ... Protocols ddPCR Titration of Lentivirus Vectors ddPCR Titration of Lentivirus...droplets (blue/green). The concentration of RRE positive droplets in the untransduced control should be close...serially diluted and used to transduce HEK293T cells. Genomic DNA is extracted from the target cells and ...+06 7 Untransduced 1 6.4 17940 0.0007134894091 1.43E+02 Table: Example dilutions and titrations Reference...lentivirus vector titration. Protocols...titer range of 1E+05–1E+09 TU/mL, where TU is transducing units and generally represents the number of ...

  18. Transfection for Recombinant Antibodies

    Type
    Protocol
    ... to proceed with transfection. Transfect the flask containing 108 mL cells: Transfer 6 mL of BCD TFX into...General Transfection Protocol Recombinant Antibody Purification Protocol Introduction Transfections allow...using Polyethylenimine Max as a transfection reagent. After transfection and expression, the recombinant...Section 2: Transfection Check the cell density and viability: Using a 5 mL pipette, transfer 0.5 mL of ...feeding schedule: Thursday: Transfect cells. Friday (24 h post-transfection): Add 4% BCD Feed. Monday (...post-transfection): Add valproic acid to 3.75 mM, add 4% BCD Feed. Tuesday (120 h post-transfection): Add...Wednesday (144 h post-transfection): Add 4% BCD Feed. Thursday (168 h post-transfection): Harvest antibody...

  19. Kit Free RNA Extraction

    Type
    Protocol
    ... Protocols RNA Extraction Without a Kit RNA Extraction Without a Kit...effectively lyse open the cells. Extract RNA from the homogenized sample(s).Transfer tissue/cell lysate to a 4....5 M LiCl solution to bring the concentration of LiCl in the extracted aqueous layer to 2.5 M. Incubate...perform RNA extraction without a kit. Protocols...Reference Introduction This protocol describes how to extract RNA from tissues or cells without a kit. The general...include homogenization/lysis of cells or tissues, extraction of RNA, precipitation, and resuspension. This...

  20. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...Iodixanol Gradient Ultracentrifugation AAV Purification by Iodixanol Gradient Ultracentrifugation You may also...medicine, iodixanol can be used as an intravenous isomolar contrast agent. In research, iodixanol is used...and concentration Note: Both steps could be completed in one (long) day. Equipment Ultracentrifuge T70i...the column. We recommend concentrating to a minimum of 500 µL. If the concentrate volume is less than 500...Follow this protocol using Iodixanol gradient ultracentrifugation to get pure adeno-associated virus (AAV) ... pooled for dialysis. Pool clean fractions. Concentration and buffer exchange: Stocks of Poloxamer 188...Data Figure 1: Iodixanol gradient before ultracentrifugation. The steps are clearly distinguishable thanks...
Showing: 181 - 200 of 1064 results