...undergo quality control, including AAV titration by ddPCR, in vitro and (when possible) in vivo testing, and...

...Q157TAG Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO MBP-MEK1 XX Amp) Plasmid 68300 MBP-MEK1...

...from the Abudayyeh-Gootenberg lab. A one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for reagent...

...suitable for replication in many gram-negative hosts. pCri System Various Plasmids T7/lacO and lacO variants...

... is necessary for Cas enzymes to bind target DNA PCR P olymerase C hain R eaction; Used to amplify a specific...

...coupled receptors, or GPCRs. The largest class of cell surface receptors, GPCRs are seven-pass transmembrane...receptors? Read the GPCRs: How Do They Work and How Do We Study Them? blog! Studies of GPCRs led to development...activates G-proteins to modulate downstream signaling. GPCRs have been shown to be involved in a wide variety...inflammation and neurotransmission. This, and the fact that GPCR ligands can have high potency, which is potentially...that couples to β-arrestin to activate noncanonical GPCR signaling independent of G proteins. This DREADD... manipulate neuronal activity indirectly through GPCR signaling, another class of chemogenetic receptors...

...Ultracentrifugation AAV Titration by ddPCR AAV Titration by qPCR Using SYBR Green Technology References...