We narrowed to 724 results for: rel

...sensor (MARIO) A Transient Rise in Free Mg 2+ Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic ...through membrane lipid peroxidation, cytosolic release of CAVIN1, and downstream regulation of NRF2. bioRxiv...Ultrafast glutamate sensors resolve high-frequency release at Schaffer collateral synapses. Proc Natl Acad...sensors for monitoring spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566. Yulong Li Serotonin...-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(...

...Maizel 1000000151 CRISPR-Cas9 Gateway System for Physcomitrella patens System for genome editing of the moss...seed and other stocks of Arabidopsis thaliana and related species. The Arabidopsis Information Resource (..., computational and experimental resources that relate to the control of gene expression in the grasses...

...interactions using fluorescence microscopy. FRET relies on the principle that when a donor fluorophore ...for a selection of mammalian and bacterial FRET-related vectors and standards. The Hoschschild Bacterial... that vary in strength and that can be used to reliably drive expression of a gene of interest in E. coli...

...impenetrable by a needle, and must be able to close securely. Biohazard signs should be placed in all areas...

...insert donor DNA at “landing sites” defined by relatively short sequences. Some have been paired with CRISPR...high-fidelity homology directed repair (HDR) pathway. HDR relies on copying DNA from a matching template to accurately... transduced cell population, you will look for relevant hits using NGS technology. For more detail on ...As this reporter is degraded by Cas12a, it will release a quantifiable signal that indicates the presence... J., & Van Der Oost, J. (2012). CRISPR immunity relies on the consecutive binding and degradation of negatively...

...20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-1-20297P 20297-AAV1 pAAV-EF1a-double ...20071P 20071-AAV9 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-9-20297P 20297-AAV9 pAAV-EF1a-double ...Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda AV-5-20949P 20949-AAV5 pAAV-double floxed-eNpHR-EYFP-WPRE-pA...

...Addgene's collection of synthetic biology plasmids related to Networks and Gene Regulation...Addgene's collection of synthetic biology plasmids related to Networks and Regulation. The collection includes...

...dimensional structures of proteins of medical relevance, and place them in the public domain without restriction...TEV cleavage, Bac-to-Bac Expression System, AmpR Related Resources SGC: www.thesgc.org SGC Toronto: www....

... Note: This step causes the bacteria to lyse, releasing their contents, including plasmid DNA, into solution...

...support educators interested in integrating MDH-related research into their teaching laboratories. They... plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression, structure ...

...transcriptase. Engineering of the PE enzyme and related components have helped to improve the efficiency...the reverse transcriptase. Many PE enzymes and related tools are available: PE2 — introduction of five...

...101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related protein LC3 B Autophagosomes 101784 SLC25A17-mEGFP...junctions 107579 RAB5A-mEGFP AICSDP-39 mEGFP Ras-related protein Rab-5A Endosomes 107580 AAVS1-mTagRFPT-...

...lineage tracing in multicellular systems typically relies on genetic markers, somatic mutations, or labeling... previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled...

...hundreds of available enzymes, many of which are relatively cheap. They also cut specific target sequences...and choice of restriction site. Because of the relatively long stretches of base pairing in the annealed...called TOPO cloning or TA cloning, is a method that relies on the hybridization of the complementary base ...Independent Cloning Ligation Independent Cloning (LIC) relies on the 3' to 5' exonuclease activity of T4 DNA ...

... practice this isolation is only feasible for relatively short DNA molecules. To isolate a particular ...division. Because bacteria are easy to grow, divide relatively quickly, and exhibit exponential growth rates...most plasmids are replicated in E. coli and are relatively small (∼3,000–6,000 base pairs) to enable easy...specialized for protein expression for additional strain-related information and a more extensive strain list. Strain...endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA EPI300 LGC Biosearch Technologies; MilliporeSigma...recA1 endA1 gyrA96 thi-1 hsdR17(rK- mK+) supE44 relA1 Δ(lac- proAB) [F traΔ36 proAB lacIqZΔM15] NEB Stable... ∆lacX74 galK16 galE15 e14- Φ80dlacZ∆M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 ∆(mrr-hsdRMS-mcrBC...

... piñata burst, value was subjectively ascribed relative to the basic needs of each individual; the  community... rational sgRNA design is not possible without relying on some kind of pre-analysis. At the end of 2014... Advanced functions: These functions are not entirely necessary but should be considered extremely useful...microhomology aware”. Trends: Fewer tools are being released with advanced functionality, it seems these kinds... tools dominate the software space but generally rely on less powerful algorithms to detect off-targets...to specify an organism. They do this because they rely on pre built indices or databases in order to find...

... NOT Cre No Dual recombinase-dependent: Rabies-related Addgene ID Plasmid Logic Sites and Mutations In-Stock... of Parvalbumin-Expressing GABAergic Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs...

...pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Karel Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell-3 Dlx...H134R-tdTomato CAG ChR2/H134R tdTomato Constitutive rg* Karel Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry...

...years later, PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve...developed CRISPR editing technologies that do not rely on NHEJ or HDR: Base editors : Base editors directly...

...disease-associated genes. Gene Therapy Due to their relative safety and ability to transduce non-dividing cells...lentiviral gene therapies have been shown to be relatively safe and effective. Viral Vector Safety The two...Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR in cis...HEK293T cells will produce the viral vectors and release them into the supernatant. After an initial media...particularly effective for stem cell therapies, which rely on slowly proliferating cell populations that can...institution's best practices with regard to lentiviral-related research. See Addgene’s Biosafety Resource Guide....72.11.8463-8471.1998 PMID: 9765382 Durand, S., & Cimarelli, A. (2011). The inside out of lentiviral vectors...