...NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid 2 like 2 NFKB1 Nuclear factor of kappa light polypeptide...Ral guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor...GTPase and is a member of the G protein (guanine nucleotide binding protein) family. GTPases serve as molecular...activating protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin... RASGRF1 RASGRF2 Ras protein specific guanine nucleotide releasing factor RASGRP RASGRP1 RASGRP2 RASGRP3...scaffold protein SOS SOS1 SOS2 SOS Ras/Rho guanine nucleotide exchange factor SPRED SPRED1 SPRED2 SPRED3 Sprouty...TSC1 TSC2 Tuberous sclerosis VAV1 Vav guanine nucleotide exchange factor 1 YAP1 Yes associated protein...

...eukaryotic process where approximately 20-30 nucleotides of double-stranded RNA (dsRNA) results in downregulation...functional APT will convert 2-FA to cytotoxic nucleotides, resulting in death. In the presence of 2-FA,...

...hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger nucleases, and many others (12). The...

...targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - ...detect nucleic acids at the atomolar level. For more information read Addgene's Blog: Finding nucleic acids...monoclonal antibodies that target SARS-CoV-2 nucleocapsid protein. SARS-CoV-2 Plasmids : Plasmids that...COVID-19 research. Antibodies Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse...monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein (Brian Geiss). Plasmids SARS-CoV-2 Plasmids...degradation to develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR). Mammoth...

...antibody to detect various targets, such as proteins, nucleic acids, or glycans. Fluorophores can also be covalently...staining should have high affinity and specificity to nucleic acids, with minimal background staining. Additionally...

...off-target editing is a major concern, Cas9-gRNA ribonucleoprotein (RNP) complexes are advantageous due to the... to generate transgenic embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and ...increase or decrease depending upon the specific nucleotides within the selected target sequence. For example...example, gRNA targeting sequences containing a G nucleotide at position 20 (1 bp upstream of the PAM) may...be more efficacious than gRNAs containing a C nucleotide at the same position in spite of being a perfect...products are denatured-renatured, treated with a nuclease that cleaves DNA heteroduplexes, and run on an...restriction digest (for HDR repaired DSBs): For small nucleotide edits that introduce a novel restriction site...

... PCR efficiency from a single reaction set-up. Nucleic Acids Res. 31:e122.Thelwell N; Millington S; Solinas...application of Scorpion primers to mutation detection, Nucleic Acids Research, Volume 28, Issue 19, 1 October ...

...40822 EGFP-alphasynuclein-WT SNCA GFP CMV Parkinson's David Rubinsztein 40823 EGFP-alphasynuclein-A53T SNCA... pHM6-alphasynuclein-WT SNCA His, HA CMV Parkinson's David Rubinsztein 40825 pHM6-alphasynuclein-A53T ... Alpha-synuclein SNCA T7 Parkinson's Michael J Fox Foundation MJFF 51486 pET21a-alpha-synuclein SNCA T7...Alpha-synuclein SNCA T7 Parkinson's Michael J Fox Foundation MJFF 89074 pD454-SR rat Alpha-synuclein SNCA...Alpha-synuclein SNCA T7 Parkinson's Michael J Fox Foundation MJFF 89076 pD434-SR rat Alpha-synuclein SNCA...89470 VN-alpha Synuclein SNCA Venus CMV Parkinson's Bradley Hyman 89471 alpha Synuclein-VC SNCA Venus ...89483 VN-beta Synuclein SNCB Venus CMV Lewy body dementia Tiago Outeiro 89484 beta Synuclein-VC SNCB Venus...

...site sequence can be added by inserting an oligonucleotide linker into BsaI digested vector. Cloning targeting... of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue... 46294 pBS-Hsp70-Cas9 : A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.... 45945 pHsp70-Cas9 : The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter...

...reports have shown that engineered zinc finger nucleases (ZFNs) are effective tools for making targeted...the spacer sequence between the half-sites (see Nuclease Expression Vectors for more details). IMPORTANT...open-source' engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder...mutagenesis in zebrafish using customized zinc-finger nucleases. Foley JE et al. Nat Protoc. . 2009. Dec 3. 4(...

...predict gRNA targets in gene families, HDR oligonucleotide design, and batch processing for designing ...sequence or list of sequences. RGEN (RNA-guided endonuclease) Tools: Cas-OFFinder & Cas-Designer From the...Targeter Originally developed to identify zinc finger nuclease sites, this tool from the Joung Lab has been expanded...an accession number, genomic location, pasted nucleotide sequence, or a sequence text file you upload....

...enzyme cloning, TA cloning, or annealing of oligonucleotides. These entry vectors are used in a Golden ... Liu and colleagues, FokI-dCas9 expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding...

...3;108(18):7351-6. Amy Palmer Zinc Cytosolic or Nuclear zinc-binding FRET biosensor (Zap) New alternately...bacterial or mammalian expression, targeted to nucleus, cytoplasm, or mitochondria A genetically encoded...Photosynthesis-dependent H 2 O 2 transfer from chloroplasts to nuclei provides a high-light signalling mechanism. Nat...cellular concentrations of nicotinamide adenine dinucleotides. eLife. 2018 May 29;7. pii: 32638. Kai Johnsson...GRAB-HTR6 A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility. Cell... protein pH biosensor to detect concentrative nucleoside transport. J Biol Chem. 2009 Jul 31;284(31):20499...

...repeatedly the reactive groups on a nucleotide when adding a nucleotide one at a time. The main property ...more than one nucleotide at that position termed a mixed site. One can also use a nucleotide-based molecular... Gel Electrophoresis Primer Design for PCR Oligonucleotide primers are necessary when running a PCR reaction.... They are synthesized chemically by joining nucleotides together. One must selectively block and unblock... the primer usually has stretches of several nucleotides. Also, both of the 3’ ends of the hybridized ...process. When designing, if unsure about what nucleotide to put at a certain position within the primer...molecular insert (inosine) instead of a regular nucleotide for broader pairing capabilities. Taking into ...

...associated reverse complement including additional nucleotides for cloning and expression purposes. NOTE: Here... BbsI restriction enzyme ( Table 3 ). Add a G nucleotide after the CACC sequence and before the 20-mer...pX330 vector is enhanced by the inclusion of a G nucleotide after the CACC sequence. Add a C at the 3’ end...Ligation Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated...

...will include the free nucleotide dGTP in the reaction (exclude all other nucleotides from standard polymerase...without ligation. Because of its dual polymerase/exonuclease functions, T4 DNA polymerase can create overhangs... which a single dNTP is included to stop the exonuclease function of the enzyme and shift its activity...polymerase protocol), causing the enzyme to perform exonuclease activity until the first "G" in the sequence.... the polymerase reaction is favored over the exonuclease reaction, the polymerase will add back the guanosine...manufacturer. It is very important to remove all free nucleotides from your PCR product before proceeding, as they... they will interfere with the exonuclease activity of T4 Pol in the following step. This may be accomplished...

...EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid for...design tools and ordered as standard desalted oligonucleotides with the appropriate overhangs: The plasmids...quick ligation of annealed and phosphorylated oligonucleotides containing the guide target sequence: Up to...

...recognizable sub-cellular localization (membrane, nucleus, etc.) are also available Non-toxic to living cells...pSNAPf-H2B Control Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid...pCLIPf-H2B Control Plasmid CLIP-tag Control for nuclear localization 101130 pCLIPf-Cox8A Control Plasmid...

..., additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can...or Cas9 domains PE7 - addition of RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA...

...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan...