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  1. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...bacterium picking up resistance genes from it’s environment (from DNA left behind after another bacterium...survival. Existing virtually ubiquitously in the environment, there is an intense amount of competition between...

  4. Lab to Office Culture Shock

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    Blog Post
    ...it’s like to be properly onboarded. Most work environments outside of academia provide a formal training... until I was out of the lab and in an office environment. Ultimately, it will likely be these skills that...

  5. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...from GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across...scientists with the ability to selectively silence neurons in vivo (Slimko and Lester, 2003).  Addgene has...

  6. Hot Plasmids - August 2020

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    Blog Post
    ...- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over conventional antibodies...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...

  8. Tips for Writing a Good Cover Letter

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    Blog Post
    ...reproducible assay. That information can make you a much stronger candidate!  Additional uses The cover letter is...job with a lot of file management that asks for strong organizational skills. You might think, “I’d be...

  9. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post
    ...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...the chance of the insert being “dropped” in the wrong place, while increasing the number of potential ...

  11. Hot Plasmids: Fall 2024

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    Blog Post
    ...Epitope tag antibody collection grows By Ashley Waldron We are excited to highlight the Anti-V5 tag antibody...codon-optimized for C. elegans and included artificial introns to improve expression. When using these constructs...

  12. Tips for CRISPR Gene Editing in Mice

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    Blog Post
    ...however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice....Daisuke, et al. "Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9...

  13. The Challenges of Cell Culture

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    Blog Post
    ...system could be as simple as a piece of paper on the front of the incubator containing the passage number, ...ensure the results are reliable. With this two-pronged approach, the many mistakes of the past can be ...

  15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post
    ...compared to one pot saturation mutagenesis. Error-prone PCR: This method amplifies the wild-type template...Chaput, J. C. (2010). “Random Mutagenesis by Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis...

  16. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post
    ...Yiping Qi lab and the MoClo Plant Parts Kit from the Patron Lab.  As with all plasmids in the repository, we...Genome-Editing Tool: Application in Improvement of Crops. Front Plant Sci 7: . https://doi.org/10.3389/fpls.2016.00506...

  18. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post
    ... Many cell types can be found in the tumor microenvironment (tumor cells, immune cells, blood cells and...recorded events) based on their expected size. The strong signals close to the graph’s origin mainly stem...

  19. Hot Biosensors 2022: Year-End Roundup

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    Blog Post
    ...MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved sensors...Small Near-Infrared Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:880107. doi: 10.3389...

  20. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    Blog Post
    ...light-sensitive ion channels used to control activities like neuronal signaling — think of a mouse with an LED brain... — but there’s a lot more to optogenetics than neuronal activity. Researchers have designed a variety ...

  22. 10 Steps to a Perfect Science Talk

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    Blog Post
    ...Joanne's Reddit AMA 6. Practice, practice, practice in front of a real audience Good, even great, speaking skills...help you avoid giving only yes or no answers in front of a crowd. Having answer slides ready after your...

  23. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post
    ...restore the dystrophin reading frame. gRNAs bind in intronic regions, so low-frequency indels will not cause...exonic regions comes with much more risk than the intronic editing used in the exon skipping approaches here...

  24. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post
    ...excessive costs in your lab. That in itself is a strong negotiating tactic, and puts you ahead of most ... create productive and manageable laboratory environments. His business skills enhance his passion for...

  25. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post
    ...mediated dsDNA breaks: following a cut, the error-prone nature of non-homologous end joining (NHEJ) often...optimized sequence will do little if it is in the wrong place, but because the target window is more-narrow...

  26. Is this the right place for me? 8 tactics for choosing a lab

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    Blog Post
    ...reputation? If you are choosing a training lab, I strongly suggest you complete a work rotation in the lab...and non-academic career paths. I can’t say this strongly enough - don’t choose a mentor that sees you only...

  28. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post
    ...brought together and microinjected into either the pronucleus or the cytoplasm of fertilized mouse eggs. Or...remember that you are working with RNAs, which are prone to degradation by the omnipresent RNase. You may...

  29. Finding Your Science Policy Path

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    Blog Post
    ...backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering and everything...policy training programs and fellowships have a strong focus on early career researchers who have decided...

  30. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ...the labeling kinetics or the fluorophore’s microenvironment to enhance brightness, stability, or other...10.1021/ar200099f Keppler, A., Gendreizig, S., Gronemeyer, T., Pick, H., Vogel, H., & Johnsson, K. (2003...

  31. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...Plants Mediated by a Cas9 Gene Containing Multiple Introns.” Plant Communications, vol. 2, no. 2, Elsevier...10.1021/acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR...

  32. Typing CRISPR Systems

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    Blog Post
    ...extreme sequence divergence that requires a multi-pronged analysis to sort effectively. The current classification... functions within Class 1 CRISPR-CAS systems. Frontiers in Microbiology, 12. https://doi.org/10.3389/fmicb...

  36. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... and nanoscale immunolabels in mammalian brain neurons. eLife . 8:e48750. PMID: 31566565 (Link opens in...
Showing: 441 - 480 of 671 results