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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 245 results for: grn

Showing: 221 - 240 of 245 results

  1. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems...

  2. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated...

  3. Plant Plasmids and Resources

    Type
    Collection
    ...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice... different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR...Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots...

  4. Lentivirus Plasmids

    Type
    Collection
    ...plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search "lenti...

  5. Tetracycline Inducible Expression

    Type
    Collection
    ...104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA TetR H1-2O2 Nathanael...

  6. Plasmid Collections

    Type
    Collection
    ...libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids ...

  8. Microbiology Resources

    Type
    Collection
    ...CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast CRISPR resources ...

  9. Worm Expression Resources

    Type
    Collection
    ...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging...

  11. Zebrafish Plasmid Collection

    Type
    Collection
    ... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR...

  13. Rett Syndrome

    Type
    Collection
    ... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources...

  14. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...

  16. CRISPR Guide

    Type
    Guide
    ... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...conversions using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide...interactions between the gRNA scaffold and surface-exposed positively-charged grooves on Cas9. gRNA binding induces...

  17. Plan Your Experiment

    Type
    Guide
    ...edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.... system for Cas9 and the guide RNA (gRNA), the design of the gRNA sequence, and finally how to validate... proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...most cases gRNA design software is better and more efficient at designing potential gRNAs for you to choose...testing multiple gRNAs is a good idea for most CRISPR experiments, testing multiple pegRNA designs is particularly...your gRNA is the balance between on-target and off-target activity. In a perfect world, your gRNA sequence...mismatches between a given gRNA sequence and your genomic target may reduce the gRNA activity. Be sure to sequence...

  18. Guide to Using Pooled Libraries

    Type
    Guide
    ...each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains a unique gene targeting...library from the Moffat lab has over 175,000 different gRNA-containing plasmids). Amplifying and Using the Library... screen. The figure above is an outline of how a gRNA library is used. Below are the general steps to ...experimental and control cells Generate a list of gRNAs that disappear with addition of the selection mechanism...

  19. Lentiviral Vector Guide

    Type
    Guide
    ... require gRNAs for each target. Lentiviral vectors are a common way of delivering these gRNAs, due to ...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ...

  20. Modular Cloning Guide

    Type
    Guide
    ...-in-one CRISPR/Cas9 vectors expressing multiple gRNAs (up to seven) with a Cas9 nuclease, nickase, dCas9...plasmids including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for genome editing in monocots...
Showing: 221 - 240 of 245 results