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  1. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...on the Addgene blog and have created and updated guides on various topics in biology over the past year...containing these fluorescent proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview...

  2. Editor's Choice, August 2016

    Type
    Blog Post
    ...choice picks this month, I’m picking two posts that describe methods to make single base edits in DNA. The ...from Cornell University, is a bit old school and provides a thorough walkthrough of how you can use PCR ...

  4. Synthetic Biology - Algal

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  5. Synthetic Biology - Bacterial

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  6. Synthetic Biology - Fungal

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  7. Synthetic Biology - Mammalian

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  8. Synthetic Biology - Plant

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  9. Synthetic Biology - Worm

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  10. Synthetic Biology - Yeast

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Do you...

  11. Tags and Other Markers

    Type
    Collection
    ...our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity Recommended...

  12. Synthetic Biology - Strains

    Type
    Collection
    ...available from this depositor's lab. Plasmid Description Gene/Insert Antibiotic Resistance PI Publication...

  15. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...wall of green algae (Soo et al., 2015). There, it divides into two cells. At the same time, it sucks out ...1983). Grasshopper parasite The parasitic worm Spinochordodes tellinii‘s larvae makes its home inside grasshoppers...

  16. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...interactive game that Addgene employees created and designed for the Hall of Human Life at the MOS. (If you...all the fixings), as well as plenty of delicious desserts. We enjoyed a video highlighting the changes at...

  17. Neuroscience

    Type
    Collection
    ...our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity Recommended...

  20. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...new Help Center! Created using the same reliable Zendesk system that powers our email support, this portal...your name and email address, after which you can describe your issue and press send. Doing so will bring...

  21. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...Improving Agrobacterium binary vectors Vectors designed for use in Agrobacterium are termed binary vectors...ideal for large gene transfer. The M24 promoter provides constitutive, high-level gene expression and is...

  22. Zinc Finger Consortium Reagents

    Type
    Collection
    ...and educational use. Links to additional pages describing these reagents are provided below: Zinc Finger...

  23. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive...

  26. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...repository and serving scientists better. That includes keeping fees as low as possible to ensure that...processes, distribution, and customer service) Addgene provides unmatched customer service that is consistently...

  30. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ... favors the breakage of the hydrogen bonds, destabilizing the molecule and favoring the separation of ...nuclease free water is a great option because it provides a great versatility on how to use your plasmid...

  31. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...camelids too, though not just for the reasons the UN describes. In honor of the Camelid family, I wanted to take...of times on this blog over the years and have described how they compare to other antibodies and affinity...

  32. Hot Plasmids - October 2022

    Type
    Blog Post
    ...optimized for 2-photon imaging of voltages. This includes monitoring of fast bursts, as well as slow up/...KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that...

  34. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...come. Some Freshers Fairs can include fairground rides and other fun activities and even invite members...its members to become involved with. The society provides talks and career support for a range of different...

  35. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...transposon system is one such system which has been designed to perform gene transfer in vertebrates. The system...whose use is only likely to grow in the coming decades.  References 1. Kowarz E, et al. Optimized Sleeping...

  36. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...the measure of work is not just time spent at the desk, but it is worth it. This requires a focus on deliverables...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...

  37. Advice for Choosing a Research Project

    Type
    Blog Post
    ...good and bad data, and knowing how to follow the design, execute, analyze, repeat pathway that is required...critically before diving in. Research projects are designed to train graduate students and postdocs to think...

  38. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...when the cells are grown on selective media, and provides those bacteria with a pressure to keep your plasmid...gene and acts as a marker for the mutation. When designing these types of experiments it is best practice...

  39. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...PDF at the end of this blog post. This document includes a list of questions to ask your potential advisors...some good pre-work you can find a lab that will deserve your hard work and an advisor that will be a lifelong...

  40. KLF Research Plasmids

    Type
    Collection
    ...Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...

  42. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...the Addgene website. blog.addgene.org The blog provides tips for working with viruses including advice...on each viral prep’s specific material page and includes western blots as well as other expression data...

  46. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...without end-point sequencing assays.  This tool was designed by putting together the coding sequence of a mCherry...MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent...

  48. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...
Showing: 601 - 650 of 983 results