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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 716 results for: cas

Showing: 241 - 260 of 716 results

  1. Adenovirus Plasmids

    Type
    Collection
    ...deletion in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral A block...deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle For production of...Shuttle Modified pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids Zhang 50957...

  2. Antibody Guide

    Type
    Collection
    ...expression. Monoclonal antibodies are necessary in cases where extreme specificity is important. Their epitope...when epitope specificity is not a concern or in cases where you may want to identify or collect the protein...azide, as it acts as an inhibitor to HRP. In those cases, thimerosal can be used as an antimicrobial agent... using the above process and try again. In some cases, it may be preferable to buy small aliquots of many...

  3. The Pleiades Promoter Project

    Type
    Collection
    ...webpages. These plasmids are described in Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters MiniPromoter...VIP pEMS1722 intron-lacZ/NLS References Portales-Casamar, E., Swanson, D. J., Liu, L., de Leeuw, C. N., ...Bonaguro, R. J., Brauer, E., Candido, T. R., Castellarin, M., Chen, J., Chen, Y., Cheng, J. C., Chopra...

  4. Bacterial Expression Systems

    Type
    Collection
    ...protein expression in the stationary phase. In these cases, you may want to control protein levels or to turn...Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia...synthesize a target metabolite, genetic tools like CRISPR-Cas9 are not only valuable but often essential. Find ...

  5. Plasmids for Stem Cell Research

    Type
    Collection
    ...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts...CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice...

  6. Brain Initiative Collection

    Type
    Collection
    ...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV9 pAAV-Ef1a-DIO-PPO-Venus...photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1 pAAV-EF1a-iCreV can...

  7. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...titered by droplet digital PCR (ddPCR) . In some cases, we may expect lower titers for a particular AAV...titer due to toxicity or other reasons For these cases, Addgene will promptly notify you by email within...

  9. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...Site (5' to 3'; half-sites in CAPS, spacer in lowercase) aak1 TAL3208 & TAL3209 TCTTTGACTCCCGGAGAGagctggtgggctccgggCCAGGTTCTGGAGGGGGA...TGGACGCGGAGCTGTTCTcggagcaggagctgacCTGCTCCATCTGTCTGGA casp8ap2 TAL3038 & TAL3039 TATAACAAATCCCACCACattgcccgatatcttgAATACTTCCAAAACAAAA...

  11. Distribution to Industry

    Type
    Collection
    ...Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53...

  12. Arf GTPase Family

    Type
    Collection
    ...Link opens in a new window) Sztul, E., Chen, P., Casanova, J. E., Cherfils, J., Dacks, J. B., Lambright,...

  13. Viral Production

    Type
    Collection
    ... contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event of contamination...

  14. Ras Pathway

    Type
    Collection
    ...and differentiation. Schematic of Ras signaling cascade that starts at the plasma membrane. Cancer Impact...

  15. TALEN Plasmids and Kits

    Type
    Collection
    ... 21179091), as well as (v) distinct selection cassettes on pTAL7a and pTAL7b for enrichment of double-...

  16. Retrograde AAV viral preps

    Type
    Collection
    ... of the final viral vector preparation. In some cases, in vivo testing of these viral preparations was...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...either Neo or Hygro resistance markers with CRISPR/Cas vectors targeting their genes of interest to generate...CRISPR-Cas9-based tool for targeting CpG methylation by fusing catalytically inactive Cas9 (dCas9) with...pDGE Dicot Genome Editing Kit contains Cas9, Cas9 nickase, and Cas9 activator expressing plasmids that can...high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans CRISPR/Cas9 technology has revolutionized...reported on a novel Cas9-based activator developed by the Church lab, termed “dCas9-VPR”, which facilitates...creating a new dCas9-VPR expression plasmid that uses the piggybac transposase to integrate dCas9-VPR into ... is genomic integration of dCas9-VPR and controlled expression of the dCas9-VPR transgene by doxycycline...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... potential applications. Polstein LR & Gersbach CA, Nat Chem Biol 2015 Mar;11(3):198-200. SunTag system...fully functional Cas9 upon dimerization. Splitting wild-type Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal...specific genes using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(N)-FRB-NES). This...gene activation when fused to dCas9. In the dCas9-SunTag-VP64 system, dCas9 is fused to a scaffold containing...domains onto dCas9 to generate a dCas9-VP64-p65-Rta fusion protein (referred to as dCas9-VPR) had a synergistic... can write a blog post about it! Split Cas9 system The Cas9 protein is composed of an n-terminal DNA... the bi-lobed architecture of Cas9 to engineer a series of "split" cas9 molecules that cannot function...

  20. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... gRNA, Cas9 binds the target site, but does not cut either strand of DNA. Traditional Cas9 regulators...capability, and this nuclease-null or “dead” Cas9 (dCas9) variant can then be fused to effector domains...importantly, most Cas9 orthologs are less well characterized than the conventionally used SpCas9 (derived from...to exhibit more limited activity than SpCas9 (2). In many cases this is due both to lower relative nuclease... a potent Cas9-based transcriptional regulator without making any functional changes to Cas9. For instance...repression with nuclease-competent Cas9. When complexed with a truncated gRNA, Cas9 is still able to bind DNA ...on rare occasions truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make...
Showing: 241 - 260 of 716 results