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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 881 results for: NSI

Showing: 651 - 700 of 881 results

  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...into the mouse zygote (Qin et al., 2015).  When inside the zygotes, the gRNA will seek out its target ... Ong ST, Bergstrom DE (2018) Viable Mice with Extensive Gene Humanization (25-kbp) Created Using Embryonic...

  4. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... that have negative impacts on fitness. One consideration when making a transgenic cell line with the ...the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon system can...

  5. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... Summers, 1998). Multimeric plasmids are also considered to be less stable and more prone to being lost...the supercoiled plasmid monomer, and at <6 kb, consistent with the size of a supercoiled plasmid dimer....

  6. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...regions you want to evolve. With this added layer of insight, you can shrink your mutant library and increase... Biotin. The antibodies developed by IPI are extensively characterized and validated, with the application...

  7. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...training. Similarly, there are several factors to consider when selecting a postdoc mentor and lab. These... having sufficient data to write a manuscript. Consider questions like: What figures are missing? For ...

  9. Colony Formation Titering Assay

    Type
    Protocol
    ... in the lab by heating to 56 °C for 30 min. Considerations Before You Start The health of the target cell...pipetting or inverting. Aliquot 1.35 mL of the cell suspension into each well of the 6-well dish directly on... 48–72 h. Pro-Tip Different cells may be more sensitive to lentivirus or to polybrene, in which case this...

  11. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ... In research, iodixanol is used as an isomolar density gradient medium suitable for virus purification...to mammalian cells. Therefore, and unlike other density gradient media, it is reportedly not necessary ...steps are used to remove contaminants with lower densities, including empty capsids. The 60% step serves ...

  14. Cloning

    Type
    Guide
    ...vectors and recombination enzymes can be quite expensive. In addition it is quite difficult to switch to...

  15. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ..., be aware that some gloves may contain latex. Consider nitrile gloves as an alternative. For extra precautions...anyone else associated with Addgene, can be held responsible for your use of the information contained in ...

  16. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...satellite colonies will grow). It is, however, more expensive. With a 6 cm diameter petri plate containing solidified...Plate 50 µL of transformed E. coli /rescue media suspension onto the agar and gently spread over the surface...

  17. Centrifugation

    Type
    Protocol
    ...from a liquid or separate liquids of different densities is essential to many different experimental procedures...samples. If your samples are significantly higher density than water, then you may need to fill the blank...

  18. Gibson Assembly Protocol

    Type
    Protocol
    ...and molecular biologists now use this method extensively. Why Gibson Cloning? No need for specific restriction... Assembly master mix (isothermal reaction mix) consists of three different enzymes within a single buffer...

  19. General Transfection

    Type
    Protocol
    ...linear polyethylenimine. Transfections allow for transient expression of a gene of interest in a target cell...discard the tube and thaw a new working stock. Considerations Before You Start The health of the cell line...

  20. Immunocytochemistry

    Type
    Protocol
    ...well. Allow the HeLa cells to grow to the desired density before labeling. Section 2: Fixing and permeabilizing...instructions before starting your experiment and consider titrating your antibody to determine the optimal...

  21. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ... 96-well optical plate Pipette tips General Considerations Always run standards and samples in duplicate... #59462 is one plasmid that gave reliable and consistent results. Use the recommended plasmid, or test...

  22. Pouring LB Agar Plates

    Type
    Protocol
    ... bath Pipetman Reagents 37 g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein...colonies will grow). However, it is also more expensive. Procedure Measure 37g of pre-mixed LB-agar powder...

  23. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...After staining with Coomassie blue, protein band intensities are measured using ImageJ software and a standard...similar photo software to determine the relative intensity of the protein bands to the overall lane. Import...

  24. Western Blot

    Type
    Protocol
    ...blocking buffer and optimal antibody concentrations. Consider titrating your antibody to determine the optimal...instructions before starting your experiment and consider titrating your antibody to determine the optimal...

  25. CRISPR Library Amplification

    Type
    Protocol
    ... (QC) Pooled libraries can be challenging and expensive to ensure adequate quality, but this upfront cost...for all plates. Use two 10 mL pipettes (one for dispensing cold LB and one for removing LB containing bacteria...

  26. New Coomassie Protocol Video

    Type
    Blog Post
    ...Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol...

  27. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...updating our CRISPR guide. Our CRISPR guide is a comprehensive resource that covers most things CRISPR - from...

  36. Call for Guest Bloggers

    Type
    Blog Post
    ...Blog Possible guest topics Some topics you might consider are: An overview of software tools useful to ...

  38. Summer Fun at Addgene!

    Type
    Blog Post
    ...tackling complex questions, and giving tips for consistently generating solid data. If you’re ready for the...

  39. Our New Antibody Mascot is....

    Type
    Blog Post
    ...After an agonizing week spent in cloistered consideration, and with much debate, we have finally narrowed...

  42. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers...

  44. Editor's Choice, October 2016

    Type
    Blog Post
    ... incredibly important information for anyone considering a postdoc. In “Changing Labor Laws Bring Increased...

  46. Editor's Choice, July 2016

    Type
    Blog Post
    ... our posts if you have any questions or useful insights. If you're interested in blogging for us, fill...

  48. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    ...comments below. We’ll track down the story, get the inside scoop, and keep you up-to-date. Be the first to...
Showing: 651 - 700 of 881 results