Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 564 results for: BLI

Showing: 261 - 280 of 564 results

  2. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... As scientists, we probe beyond the realm of established theory. Realizing that textbooks don’t tell us...research in basic biology, medicine, epidemiology, public health, and more.     A few of the most common...

  4. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications... ID Plasmid Gene/Insert Selectable Marker PI Publication RNA Knockdown Type VI CRISPR systems, including...

  6. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish...Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Parasites...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...

  7. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...plasmids. Each destination vector contains GFP, enabling you to select cells with high GFP expression. ...

  8. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing...opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication RNA Targeting Type VI CRISPR systems, including...the entire population. ID Plasmid Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) ...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...very robust in this case for EMX1. Since the publication of our paper, we have two new optimized primers...primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the reaction...

  12. Rett Syndrome

    Type
    Collection
    ...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...Mouse line with conditional deletion of Xist Unpublished Joost Gribnau Human Cell Line Models Induced ...DNA) Mutation (protein) Sex Source of Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link....806delG 806delG G269Afs*20 M Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold A140V...A140V C419T A140V M Fibroblasts Unpublished (Link opens in a new window) Wendy Gold AN_BU 808delC Arg270Glufs...Arg270Glufs*19 F Fibroblasts & iPSC Unpublished (Link opens in a new window) Wendy Gold BO_DI 806delG G269Afs...G269Afs*20 F Fibroblasts Unpublished (Link opens in a new window) Wendy Gold HO_AN G917A R306H F Fibroblasts...

  13. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...When using the AAV2(Y444F) serotype in future publications, please acknowledge Arun Srivastava and cite...When using the AAV2(trpYF) serotype in future publications, please acknowledge Arun Srivastava and cite...model of complete congenital stationary night blindness. Hum Mol Genet . Nov 1;24(21):6229-39. PMID: 26310623... using the AAV2(4pMut)dHS serotype in future publications, please acknowledge Shannon Boye and cite Boye...using the AAV6(dbY-F+T-V) serotype in future publications, please acknowledge Arun Srivastava and cite....9 When using the AAV44.9 serotype in future publications, please acknowledge John Chiorini and Shannon... using the AAV44.9(E531D) serotype in future publications, please acknowledge Shannon Boye and cite Boye...

  14. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Promoter PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have...

  15. COVID-19 Resources

    Type
    Collection
    ... has published information and protocols for utilizing SHERLOCK to detect coronavirus - Enabling coronavirus...Trans Reporter (DETECTR). Mammoth Biosciences has published information and protocols for utilizing DETECTR... reporter of SARS-CoV-2 main protease Mpro. (Unpublished) A FlipGFP-based activity reporter of SARS-CoV...Qi's lab. COVID-19 Diagnostic Toolkit Enzymes. (unpublished) Taq polymerase and MMLV reverse transcriptase...Starr). External Resources Research Articles Many publishers have allowed free access to articles related ...of COVID-19 related articles spanning multiple publishers can be found here: ASM COVID-19 Research Registry...

  16. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Description Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

  17. CRISPR Plasmids - Plants

    Type
    Collection
    ... ID Plasmid Gene/Insert Selectable Marker PI Publication Base Edit Catalytically dead dCas9 fused to a...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Empty gRNA Expression Vectors Select a gRNA expression...

  18. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic cells. A common...be found at Addgene and in Figure S12 of the publication. Agudelo D, et al. Nature Methods. 2017. PubMed...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    ...validation assays. If you’re using previously published antibodies, check the manuscript and supplementary...or follow the references to find the original publication and ensure that it includes validation data relevant...

  20. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Drosophila...Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Yeast ID ...Description Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...
Showing: 261 - 280 of 564 results