We narrowed to 571 results for: BLI
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TypeBlog Post...the first paper identifying a binding receptor published this year (Dhungel et al., 2025)! It can transduce...contributing their expertise! This post was originally published in January 2025 and updated in August 2025. References...
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A Control for All Seasons
TypeBlog Post...Histochemistry & Cytochemistry, vol. 59, no. 1, SAGE Publications, Jan. 2011, pp. 6–12. Crossref, doi:10.1369/...Histochemistry & Cytochemistry, vol. 62, no. 10, SAGE Publications, July 2014, pp. 693–697. Crossref, doi:10.1369... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...the images are accessible to all viewers. Use a colorblind-friendly color palette for display (like green-magenta-blue...informative image-based figures for scientific publications. PLOS Biology, 19(3), e3001161. https://doi.... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...and incubation times. If the vendor includes publications that cited the antibody, consider reviewing ... the materials and methods sections of those publications. Finally, always verify that the chosen antibody... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...were Spanish scientists (MD, PhD and MD/PhD) established in some of the top Universities in USA and Europe...from different Spanish universities and high level public and private institutions also showed their support... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan ...experimentation. In 2014, several manuscripts published in Genetics detailed approaches in which selection... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post... As scientists, we probe beyond the realm of established theory. Realizing that textbooks don’t tell us...research in basic biology, medicine, epidemiology, public health, and more. A few of the most common... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...reagents, we scaled up these tagging experiments by assembling csRNPs in a 96-well plate format and tested parallel...Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S et al. 2017. ... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...plasmids. Each destination vector contains GFP, enabling you to select cells with high GFP expression. ... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...editing variants Four years after their initial publication, the Liu lab returned to the reverse transcriptase... -
Antibody Validation for Flow Cytometry
TypeBlog Post...manufacturer and/or in the published literature. Note that the antibody being published doesn’t always indicate... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...very robust in this case for EMX1. Since the publication of our paper, we have two new optimized primers...primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the reaction... -
Technical Design of a Western Blot
TypeBlog Post...validation assays. If you’re using previously published antibodies, check the manuscript and supplementary...or follow the references to find the original publication and ensure that it includes validation data relevant... -
15 Hot Plasmids from 2017
TypeBlog Post...factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic cells. A common...be found at Addgene and in Figure S12 of the publication. Agudelo D, et al. Nature Methods. 2017. PubMed... -
Introducing...Addgene Quick Tips!
TypeBlog Post...is below. And you’ll be able to find all published videos in our Quick Tips playlist: Want your... -
A Case Study of Addgene Bioinformatics
TypeBlog Post...processing of our sequencing data.” Recently, Seqera published a case study on how Addgene has used Nextflow ... -
CRISPR Plasmids - Mammalian Expression
TypeCollection...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications... ID Plasmid Gene/Insert Selectable Marker PI Publication RNA Knockdown Type VI CRISPR systems, including... -
Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog Post...suggestaplasmid, the name of the plasmid and a publication where we can find the plasmid. While we cannot... -
We’re Going Viral This Week!
TypeBlog Post... the sharing of reagents and data, we’re also publishing research. Addgene’s research team also investigates... -
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...Promoter Selectable Marker PI Publication Return to top C. elegans ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker ...Marker PI Publication Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Parasite ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection...