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Showing: 261 - 280 of 960 results

  3. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...indirectly modify local chromatin state. Want more information on the wide variety of Cas enzymes? CasPEDIA ...

  5. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...enough to be packaged in AAV particles. Want more information on the wide variety of Cas enzymes? CasPEDIA ...

  6. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...CD11c-. Finally, B cells are CD11c- and CD123-, forming a population in the lower left(ish) corner.  If...learning how to read a gating strategy.  Other information FACS plots often contain other data. Some will...Abdulaziz University, Kingdom of Saudi Arabia. Bioinformation, 14, 53–59. https://doi.org/10.6026/97320630014053...

  7. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ... of a viral genome). Empty viral capsids can be formed during viral production depending on the  experimental... the solution. These characteristics give us information on three important factors. What can AAV titers...requires an electron microscope, it is not routinely performed on all new viral vector preparations. Addgene ...

  8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...genes and proteins coordinate billions of neurons to form the functional networks of the human brain.     ...Buchholz, Angrand, and Stewart 1998). FLPe’s performance was further improved, by adding a nuclear localization...technology. Read the genOway press release for more information. References 1. Atasoy, D., Y. Aponte, H. H. Su...

  10. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...additional cofactors or using significantly truncated forms that limit its functionality. To make biophysical...C-terminus (or even in the middle of proteins) and performs well in many different antibody-based applications...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...indels after DNA repair. B) M. abscessus is first transformed with Cas9 on an integrative plasmid containing...resistance selection marker KanR. This strain is then transformed with the sgRNA cassette on a plasmid expressing...

  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... when bound to DFHBI. It’s also smaller than any form of Spinach - only 49 nucleotides in length - and...aptamers are used to track cellular processes by transforming cells with plasmids encoding aptamer-tagged ...UTR of mRNA that bind metabolites, leading to conformational changes that control the expression of coding...expression vectors to create a library. Bacteria were transformed, and then sorted by fluorescence-activated cell...

  12. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...causes cells that it infects to fuse together and form syncytias that cause the cells to die. Cytotoxicity... of these review articles to find tables and information on different viral membranes, tropism, and advantages...

  13. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... pDonor carries a customizable cargo gene in the form of RE-cargo-LE (RE, right end; LE, left end) pTnsABC...in E. coli BL21(DE3) as transformants failed to grow after the transformation of pTargetF into the BL21...strand breaks it introduces makes it difficult to perform two or more integrations on the genome. Most recently... This would result in the lack of recovered transformants. Our speculation motivated us to update the ... sequence in pEcgRNA. Next both plasmids are transformed in E. coli with the donor DNA for editing to ...DE3) that resulted in a lower efficiency of transformation for pTargetF series plasmids. Fortunately, ...plasmids (pDonor and pQCascade) with 4 rounds of transformation to complete the N-acetylglucosamine strain ...

  14. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...separate plasmids bind to the same RepA molecules, forming a “handcuff” structure that physically blocks replication...provide clarity, a wet lab transformation experiment will ultimately inform whether your plasmids are ...about plasmids (Shintani et al., 2015). Now, bioinformatics tools help researchers determine how compatible...

  15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ... the anti-sense strand, then the ssDNA template formed will be sense. Therefore, mutagenic oligos need... plasmid. The ssDNA template is generated by transforming a backbone phagemid vector into an E. coli strain...to remove residual starting template. After transformation and harvest, the library is ready for use. ...true if the sense strand is nicked for template formation: template will be anti-sense, primers should be...ease of use, 2) time savings, and 3) ability to perform single site or multi-site saturation mutagenesis...

  19. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this...collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones...purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that simplifies identification...identification of the transcription factor isoform in pooled screens. This collection and library can be...transcription factor open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to ...

  20. Ras Pathway

    Type
    Collection
    ... gene it can be stuck in a constitutively active form, constantly activating pathways for cell growth ...added to this list? Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this... below. For proteins with multiple subunits or isoforms, individual links to each gene page are provided...gene. For components with multiple subunits or isoforms, individual links to each gene page are provided...E2F transcription factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor EIF4EBP... SHC4 SHC (Src homology 2 domain containing) transforming protein SHOC2 SHOC2 leucine-rich repeat scaffold...Esposito Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of...
Showing: 261 - 280 of 960 results