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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 298 results for: IRES

Showing: 281 - 298 of 298 results

  4. Deep Dive: qPCR

    Type
    Blog Post
    ... practice, multiplexing is quite difficult and requires significant optimization to ensure the results...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...for more selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate...

  11. Promoters

    Type
    Guide
    ...Constitutive but requires T7 RNA polymerase Promoter from T7 bacteriophage Sp6 Constitutive but requires Sp6 RNA...eukaryotes. In bacteria, the core RNA polymerase requires an associated sigma factor for promoter recognition...

  12. Plan Your Experiment

    Type
    Guide
    ...or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing efficiency compared...improve HDR efficiency . Remember that HDR also requires donor DNA to serve as template for the repair....: Target the transcription start site. CRISPRa requires dCas9 to be fused to transcriptional activators...

  13. CRISPR Guide

    Type
    Guide
    ...indel formation compared to HDR. Prime editing requires more optimization and typically has lower efficiency...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...

  14. Lentiviral Vector Guide

    Type
    Guide
    ...as a promoter on the transfer plasmid, and this requires Tat for activation. Second-generation plasmids...vectors from your plasmids (Figure 5). This process requires a production cell line, typically human embryonic...

  15. Molecular Biology Reference

    Type
    Guide
    ... regions of interest. In vitro DNA replication requires the 4 nucleotides, a DNA polymerase enzyme, the...tagged base is incorporated. Sanger sequencing requires a lot of DNA because the ultimate goal is to have...

  16. Antibody Guide

    Type
    Guide
    ...cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires single-antibody...

  17. Modular Cloning Guide

    Type
    Guide
    ...highly-tunable GPCR-based biosensors in yeast (requires MoClo-YTK parts for use). Yeast GoldenBraid Cloning...
Showing: 281 - 298 of 298 results