...Prime editing is precise and versatile, but it requires careful optimization to achieve the best efficiency...

... practice, multiplexing is quite difficult and requires significant optimization to ensure the results...

...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. E. coli and T. citrea...

...does base editing work? Base transition editing requires three elements. They are, broadly: A Cas protein...

...concentration of your reagent. Be aware that this approach requires excellent attention to detail, since you’ll end...

...and astrocytes located in the CNS, however, it requires high doses. Requests have shifted toward the more...

...for more selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate...

...for a protein of interest (POI). This technique requires the production and expression of fusions between...

...Constitutive but requires T7 RNA polymerase Promoter from T7 bacteriophage Sp6 Constitutive but requires Sp6 RNA...eukaryotes. In bacteria, the core RNA polymerase requires an associated sigma factor for promoter recognition...

...transcription of shRNAs. cDNA expression requires RNA pol II, and thus requires an RNA pol II promoter, such as...driven by the 5'LTR, which is a weak promoter and requires the presence of Tat to activate expression. The...

...indel formation compared to HDR. Prime editing requires more optimization and typically has lower efficiency...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...

... regions of interest. In vitro DNA replication requires the 4 nucleotides, a DNA polymerase enzyme, the...tagged base is incorporated. Sanger sequencing requires a lot of DNA because the ultimate goal is to have...

...cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires single-antibody...

...supplied in trans . In addition to Rep and Cap, AAV requires a helper plasmid containing genes from adenovirus...

...targeting the region where the edit should be made HDR requires a repair template and displays reduced efficiency...