We narrowed to 299 results for: IRES

...weak, random binding to precision gene editing requires three major steps: RNA loading, DNA sequence recognition...

...Prime editing is precise and versatile, but it requires careful optimization to achieve the best efficiency...

...antigen of interest in the given protocol. This requires use of one of the five pillars of antibody validation...

... practice, multiplexing is quite difficult and requires significant optimization to ensure the results...

...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. E. coli and T. citrea...

...does base editing work? Base transition editing requires three elements. They are, broadly: A Cas protein...

...concentration of your reagent. Be aware that this approach requires excellent attention to detail, since you’ll end...

...and astrocytes located in the CNS, however, it requires high doses. Requests have shifted toward the more...

...for more selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate...

...for a protein of interest (POI). This technique requires the production and expression of fusions between...

...bacteriophage; requires T7 RNA polymerase Sp6 Constitutive Promoter from Sp6 bacteriophage; requires Sp6 RNA ...eukaryotes. In bacteria, the core RNA polymerase requires an associated sigma factor for promoter recognition...Drosophila actin 5c gene Gal4/UAS Specific Insect Requires UAS regulatory element and yeast Gal4 gene; often...

...or Cas9 fusions with proteins that promote HDR Requires a repair template Lower editing efficiency compared...improve HDR efficiency . Remember that HDR also requires donor DNA to serve as template for the repair....: Target the transcription start site. CRISPRa requires dCas9 to be fused to transcriptional activators...

...indel formation compared to HDR. Prime editing requires more optimization and typically has lower efficiency...living cells. Visualizing a specific genomic locus requires recruitment of many copies of labeled proteins...gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites...

...information on genes unless combined with perturbations Requires deep sequencing Must maintain high representation...Essential genes may complicate knockout screens Requires Cas machinery, which may need to be expressed ...

... strand. Mimicking the DNA replication process requires the four nucleotides (dNTPs), a DNA polymerase...synthesis after their incorporation. Sanger sequencing requires a lot of DNA because the ultimate goal is to have...

...as a promoter on the transfer plasmid, and this requires Tat for activation. Second-generation plasmids...vectors from your plasmids (Figure 5). This process requires a production cell line, typically human embryonic...

...cytometry requires a negative control, a small subset of the sample with no antibodies. It also requires single-antibody...

...highly-tunable GPCR-based biosensors in yeast (requires MoClo-YTK parts for use). Yeast GoldenBraid Cloning...

...central (CNS) nervous systems. Targeting the CNS requires crossing the blood-brain barrier (BBB), and a ...