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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 732 results for: SON

Showing: 451 - 480 of 732 results

  2. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...do aptamers! Corn was recently described in 2017 (Song et al., 2017). Corn naturally dimerizes, and this...PMID: 28747322. PubMed Central PMCID: PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription...

  4. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...’s software in order to generate signals in a reasonable range. If colleagues in your group already have...interest can still be gated out later. Based on personal preference, some people like to make stricter ...

  5. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...Site-Specific Genome Alterations. Edited by Doudna J and Sontheimer EJ. Methods in Enzymology Vol. 546; (2015). PubMed...inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al. Nat Biotech. 34(3); (2016). PubMed...

  6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...is also being realized. There are a variety of reasons to consider using genome editing to change the ...yellow striate mosaic virus (Gao et al., 2019) or sonchus yellow net rhabdovirus (Ma et al., 2020) have already...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...RNAs." PloS one 11.2 (2016): e0149483. Rogers, Jameson K., and George M. Church. "Genetically encoded ...-tagged human ORF clones to allow testing and comparison of different cell-free expression systems; the...

  8. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...structure associated to these tools, skills, and personnel Define pricing according to all of the needed ...managers, and techs’ financial books.  To find personnel salary ranges, go to online job information sites...

  10. Optogenetics Guide

    Type
    Guide
    ...t/t), ChrimsonR Red-shifted peak action spectra Examples: VChR1, C1V1(t/t), Chrimson, ChrimsonR, Chronos...helveticum 500 Chrimson, ChrimsonR Red-light drivable channel from Chlamydomonas noctigama . ChrimsonR carries...research, helping scientists to better understand Parkinson’s disease, depression, drug addiction, and more...Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I, Thompson KR, Deisseroth K. 2010 Molecular and cellular ...Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ...Methods. 9(2):159-72. PMID 22179551 Saunders A, Johnson CA, Sabatini BL 2012. Novel recombinant adeno-associated...21363959 Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein...

  11. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...fluor through a process known as bioluminescent resonance energy transfer (BRET). The fluor then emits light...calcium release have been made using fluorescent resonance energy transfer (FRET) probes. These probes generally...

  12. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Sleeper, M. M., Sanmiguel, J., Wu, D., Gao, G., Wilson, J. M., & Sweeney, H. L. (2008). Adeno-Associated...Feng, Y., Tabar, M. S., Metierre, C., Parsania, C., Song, X., Wang, G., Su, X.-D., Bailey, C. G., & Rasko...

  13. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan... Jason Nasse...

  14. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...surface targets by tumor cells, are a few of the reasons antibodies may result in a lack of response. Antibodies...efficient immunotherapy must be to reach a flexible personalized treatment, and not universal correction of cancer...

  16. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...J. M., Xu, A., Sisley, T. A., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Efficient C•G-to-G•C ...s41587-021-00938-z Koblan, L. W., Doman, J. L., Wilson, C., Levy, J. M., Tay, T., Newby, G. A., Maianti...

  18. Antibody Guide

    Type
    Guide
    ...using sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process...may need additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals...housekeeping genes). This allows for relative comparison of expression between different samples, by normalizing...normalizing protein expression to the controls before comparison. Alternatively, the samples can be normalized...ChIP protocols use enzyme digestion instead of sonication. This approach is gentler but results in non-...isolated and analyzed. Validation relies on size comparison of peptides. Independent antibody validation ...

  19. Chemogenetics Guide

    Type
    Guide
    ...19607790 (Link opens in a new window) Atasoy D, Sternson SM (2018). Chemogenetic Tools for Causal Cellular...Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, and Sternson SM (2011). Chemical and genetic engineering of...Ramirez MH, Hu X, Galvan A, Basu J, Michaelides M, Sternson SM (2019). Ultrapotent chemogenetics for research...6. PMID: 11402033 (Link opens in a new window) Sternson SM, Roth BL (2014). Chemogenetic tools to interrogate...26399324 (Link opens in a new window) Vardy E, Robinson JE, Li C, Olsen RHJ, DiBerto JF, et al. (2015)... Xiong M, Tao Y, Gao Q, Feng B, Yan W, Zhou Y, Kotsonis TA, Yuan T, You Z, Wu Z, Xi J, Haberman A, Graham...

  20. Adenovirus Guide

    Type
    Guide
    ... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may... Bullard, B. L., Corder, B. N., Gordon, D. N., Pierson, T. C., & Weaver, E. A. (2020). Characterization...Asiedu, C., Geisbert, J., Stanley, D., Honko, A., Johnson, J., Mulangu, S., Pau, M. G., Custers, J., Vellinga...gene and cell-based therapies in the new era of personalized medicine . Genes & Diseases, 4 (2), 43–63. https...window) Luo, J., Deng, Z. L., Luo, X., Tang, N., Song, W. X., Chen, J., Sharff, K. A., Luu, H. H., Haydon...

  21. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may...Ravin, S. S., Su, L., Theobald, N., Choi, U., Macpherson, J. L., Poidinger, M., Symonds, G., Pond, S. ...Ridder, J., Allahyar, A., Wang, W., Carpenter, E., Mason, A., Wong, G. K., Cameron, E. R., Kilbey, A., & ...Srivastava, S., Hussain, R., Banani, M. A., Day, W., Stevenson, E., Madigan, M., Chen, J., Khinder, R., . . ....

  22. Plan Your Experiment

    Type
    Guide
    .... D., Dionne, D., Burks, T., Raychowdhury, R., Adamson, B., Norman, T. M., Lander, E. S., Weissman, J....PMID: 35941224 Gilbert, L. A., Horlbeck, M. A., Adamson, B., Villalta, J. E., Chen, Y., Whitehead, E. H...s41594-023-01090-9 PMID: 37783853 Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. ...

  23. Lentiviral Vector Guide

    Type
    Guide
    ...third-generation packaging plasmids. For a summarized comparison of second- and third-generation systems, please... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may...doi.org/10.1016/j.biopha.2020.110276 PMID: 32502836 Johnson, N. M., Alvarado, A. F., Moffatt, T. N., Edavettal...
Showing: 451 - 480 of 732 results