We narrowed to 324 results for: cloning

Addgene's zebrafish resources: Find plasmids for use in zebrafish.

Use this protocol to prepare LB agar plates with antibiotic in your lab.

There are many factors to consider when choosing a fluorescent dye or label. This post walks you through them so you can select a suitable one for your experiment.

Cancer immunotherapies hold great promise for treating many different types of cancer. Learn more about this promising new way to fight cancer.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization studies or organelle labeling.

Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for your experiments.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations created by the Allen Institute for Brain Science.

Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.

Learn about the basics of molecular biology, including molecular genetics, plasmids, sequencing, and more.

Overview of Addgene's educational resources, including eBooks, science guides, videos, and protocols

Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and common uses.

Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and common uses.

Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

Read our adeno-associated viral vector guide to learn about AAV vector components, production and common uses.

Not sure where to start? Need a quick reference? Addgene's eBooks are here to help!

In our Antibody Guide, we define key terms, summarize antibody production and storage techniques, and explain common applications.

Find a list of universal sequencing primers and other common sequencing primers.