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We narrowed to 862 results for: LIC

Showing: 801 - 850 of 862 results

  2. Common Injection Routes in Mice

    Type
    Blog Post
    ...depending on researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint...

  7. Of Myc and Men

    Type
    Blog Post
    ... flexible - it could now be used in way more applications.   Fig. 1 Making a Protein Tag (Abridged...

  9. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations...

  17. Plasmids 101: Protein tags

    Type
    Blog Post
    ...deciding on which one to use will depend on your application (see table below). The antibodies available for...

  20. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...The glycoprotein will be incorporated into the replicating rabies virus envelope, travel retrogradely, and...

  29. How to Be an Excellent Trainee

    Type
    Blog Post
    ...tell a trainee is understanding and things are ’clicking’ for them. It’s often easiest to train individuals...

  30. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...understood, but some possible reasons include: Metabolic pressures - it takes cellular resources to produce...

  34. Five Popular Model Organisms

    Type
    Blog Post
    ... similar to humans. For example, due to their simplicity, yeast have become an important experimental ...

  42. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...protein composition. It is used for a variety of applications such as measuring target protein expression ...

  46. Cloning

    Type
    Guide
    ...example of a LIC protocol on our website. Ligation Independent Cloning (Figure adapted from LIC protocol ... repaired by the normal replication process. It is important to note that LIC has difficulty assembling...Independent Cloning Ligation Independent Cloning (LIC) relies on the 3'-5' exonuclease activity of T4 DNA...removes nucleotides from the end of a DNA strand. In LIC, the T4 DNA polymerase’s exonuclease activity creates...are repaired by the bacteria after transformation. LIC does not require site-specific recombination or a...rapid cloning method. So how does this work exactly? LIC depends on the addition of only one free dNTP to ...interest and then insert them into plasmids for replication and protein expression. So how do scientists ...

  47. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...potential formation of replication-competent viruses. While AAV themselves are replication incompetent, there...production of replication-competent viruses that would be able to infect cells and replicate autonomously... actively causing symptoms or replicating. Lytic cycle Viral replication process where the virus uses ...into cells. Replication-competent rAAV Mutated rAAV vector that has the ability to replicate and reproduce.... The Rep region encodes four non-structural replication proteins (Rep78, Rep68, Rep52, and Rep40), which...which are involved in genome replication and viral packaging. The Cap region encodes three structural ...virus. The ITR sequences serve as origins of replication and also act as signals for packaging. Figure...

  48. Molecular Biology Reference

    Type
    Guide
    ... Origin of Replication ( ori ) DNA sequence which directs initiation of plasmid replication (by bacteria...double-stranded DNA that typically carry genes and can replicate independently from chromosomal DNA. Although they...the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division, and each daughter cell receives...manipulating genes. For instance, most plasmids are replicated in E. coli and are relatively small (∼3000 - ...sequences for this purpose, which includes a DNA replication origin, an antibiotic-resistance gene, and a ... exists extrachromosomally in E. coli , it is replicated independently and segregated to the resulting...form, plasmids require a bacterial origin of replication ( ori ), an antibiotic-resistance gene, and at...

  49. Adenovirus Guide

    Type
    Guide
    ... DNA replication and host cell manipulation. Late transcripts are expressed after DNA replication starts...production of replication-competent adenoviruses (RCAs) that would be able to infect cells and replicate autonomously...26–45 kb in length and contain multiple heavily-spliced transcripts flanked by two inverted terminal repeats..., which has limited their use in therapeutic applications. However, this has also made them the most widely...presence of adenoviral genes E1, E4, E2a and VA for replication. For more information about AAVs, read our AAV...whereas the E1 region is essential for viral replication and therefore needs to be supplied through other...due to their well-established methods, relative simplicity of production, and higher titers. However, the...

  50. Antibody Guide

    Type
    Guide
    ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...of other antibodies. This has useful research applications, and is covered in the following Isotypes section...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments... molecules that can be distinguished in your application. The indirect detection method uses an unconjugated...
Showing: 801 - 850 of 862 results