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  1. Pouring LB Agar Plates

    Type
    Protocol
    ... Protocol Video Before you begin this protocol, watch the protocol video below to get a quick idea of ...including the identity of the antibiotic. Pro-Tip We batch label our plates with colored marker - particular...

  2. Western Blot

    Type
    Protocol
    ...antibody Day 2: Incubate with secondary antibody Video Watch this instructional video to learn how to use western...determine the optimal dose. Secondary antibodies must match the host species of the primary antibody. For example...

  3. Kit Free RNA Extraction

    Type
    Protocol
    ...solubility of the RNA. Pro-Tip To prevent overdrying, watch the pellet and carefully remove any residual ethanol...

  4. Protocol - How to Design Primers

    Type
    Protocol
    ...GCGGCG-restriction site-your sequence). Protocol Video Watch the protocol video below to learn how to design ...

  8. Weighing Reagents Protocol

    Type
    Protocol
    ...weighing boat, you can weigh out the reagents in batches. For example, if you need 300 g of sucrose, you...

  9. Water Bath Protocol

    Type
    Protocol
    ...general protocol suits almost any water bath. Video Watch the video for tips on using a water bath in the ...

  11. Fluorescence Titering Assay

    Type
    Protocol
    ...cells into each well of a 6-well dish. Prepare a batch of cells as follows: Dilute 525,000 cells into 14...

  14. Gibson Assembly Protocol

    Type
    Protocol
    ... order primers that are 60 bp long, with 30 bp matching the end of the adjacent fragment and 30 bp annealing...

  15. Immunocytochemistry

    Type
    Protocol
    ... compatible reagents. Secondary antibodies must match the host species of the primary antibody. For example...
Showing: 321 - 340 of 340 results