Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 658 results for: otos

Showing: 341 - 360 of 658 results

  1. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ... Require Ex Vivo Handling of Embryos. Current Protocols in Human Genetics 88: . https://doi.org/10.1002... CRISPR‐Cas9‐Mediated Genome Editing. Current Protocols in Mouse Biology 6:39–66 . https://doi.org/10.1002...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment...Activated By Designer Drugs) to determine the role of serotonin in movement. Follow her on twitter @katrinaboards...

  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...individual colonies growing out in the 96-well dish. My protocol with many additional tips can be found here. ...knockouts and knockins in human somatic cells. Nature Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene...

  4. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...protein to a DNA-editing enzyme to generate the prototypical base editor enzymes. The cytidine base editors...engineering using the CRISPR-Cas9 system. Nature Protocols 8:2281–2308 . https://doi.org/10.1038/nprot.2013.143...

  8. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... construct for every application. Plus, the photophysical properties of chemical dyes provide many advantages...Organic dyes are typically both brighter and more photostable than fluorescent proteins (often up to 10-fold...

  9. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ...–6 67,366 (Set A) 67,194 (Set B) Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E....E. coli Zhang N/A N/A N/A - The protospacers contained in the library represent all 4096 (4 6 ) combinations...

  10. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever...10.1016/j.cell.2014.02.001 Osuka, S., Isomura, K., Kajimoto, S., Komori, T., Nishimasu, H., Shima, T., Nureki...

  11. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...this strand is edited by base editors! PAM Protospacer-Adjacent Motif A short sequence that must be ...

  13. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ..., ABE8s resulted in 1.5-fold more editing at protospacer positions A5-A7 and 3.2-fold more editing at ...Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki...

  14. Antibodies

    Type
    Collection
    ...production process by sharing our protocols and quality control methods. Learn More Guide ...Recipient Instructions Addgene Help Center Antibody Protocols Looking for Antibody Plasmids? Browse our collection...

  15. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR...change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible...

  18. DNA Service - Cloning Grade DNA

    Type
    Collection
    ... and 260/280 ratio has been determined by spectrophotometer. Other methods of quantification may yield...or scientific support? Please see our Addgene Protocols for resources you may find helpful. Our Scientific...

  20. Mammalian RNAi Tools

    Type
    Collection
    ...Resources pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral packaging plasmids Web...
Showing: 341 - 360 of 658 results