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...possible in plants outside of limited, single cell protoplast analysis. Instead, microscopic plants must be...organisms with functional APT will convert 2-FA to cytotoxic nucleotides, resulting in death. In the presence...

...protein to a DNA-editing enzyme to generate the prototypical base editor enzymes. The cytidine base editors...engineering using the CRISPR-Cas9 system. Nature Protocols 8:2281–2308 . https://doi.org/10.1038/nprot.2013.143...

...donor DNA design, and implemented our optimised protocols into a medium-throughput pipeline for epitope ... mammalian cell types. A detailed step by step protocol for the csRNP method is described in our recent...

...passion for photosynthetic communities, microbial interactions, and science education. Photo cred Alina...

... construct for every application. Plus, the photophysical properties of chemical dyes provide many advantages...Organic dyes are typically both brighter and more photostable than fluorescent proteins (often up to 10-fold...

...excitation of the fluorophore and emission of a photon) of the donor can be measured using fluorescence... standards Thaler et al. (2005) Quantitative multiphoton spectral imaging and its use for measuring resonance...standards and fusion proteins Feldmann et al. (2023) Protocol for deriving proximity, affinity, and stoichiometry...

...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification Methods...binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication. This...Special considerations Many of the steps in this protocol vary depending on assay and microscopy techniques... may be a fluorophore or an enzyme. The basic protocols and controls follow that of IF (above). Sample...

...upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads to...opens in a new window) Research workspace on Protocols.io (Link opens in a new window) . This resource...

... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...this strand is edited by base editors! PAM Protospacer-Adjacent Motif A short sequence that must be ...

...important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever...10.1016/j.cell.2014.02.001 Osuka, S., Isomura, K., Kajimoto, S., Komori, T., Nishimasu, H., Shima, T., Nureki...

...but the good news is that you only need one. ProtospacerWB was developed with the purpose of applying CRISPR..., online), DESKGEN (commercial, online), or ProtospacerWB (academic, offline). While it remains to be ...

..., ABE8s resulted in 1.5-fold more editing at protospacer positions A5-A7 and 3.2-fold more editing at ...Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki...

...production process by sharing our protocols and quality control methods. Learn More Guide ...Recipient Instructions Addgene Help Center Antibody Protocols Looking for Antibody Plasmids? Browse our collection...

...change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...

...open-access articles, protocols, and other COVID-19 resource collections. Protocol: RNA Extraction Without...

...Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled Library...cellular programming or cancer drug resistance. Protocols & References A transcription factor atlas of directed...

... and 260/280 ratio has been determined by spectrophotometer. Other methods of quantification may yield...or scientific support? Please see our Addgene Protocols for resources you may find helpful. Our Scientific...

...various experimental conditions. Protocols Written and video protocols for how to use your viral prep and...

...Resources pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral packaging plasmids Web...

...GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1 Also known as MAPKAP1; Mitogen-activated...eta PKC iota PKC theta PKC zeta Protein kinase C Protor Also known as PRR5; proline rich 5 PTEN Phosphatase...