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We narrowed to 660 results for: otos

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Showing: 341 - 360 of 660 results

  1. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...protein to a DNA-editing enzyme to generate the prototypical base editor enzymes. The cytidine base editors...engineering using the CRISPR-Cas9 system. Nature Protocols 8:2281–2308 . https://doi.org/10.1038/nprot.2013.143...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment...Activated By Designer Drugs) to determine the role of serotonin in movement. Follow her on twitter @katrinaboards...

  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...individual colonies growing out in the 96-well dish. My protocol with many additional tips can be found here. ...knockouts and knockins in human somatic cells. Nature Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene...

  4. Antibody Guide

    Type
    Collection
    ...applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification Methods...binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication. This...Special considerations Many of the steps in this protocol vary depending on assay and microscopy techniques... may be a fluorophore or an enzyme. The basic protocols and controls follow that of IF (above). Sample...

  5. Neurodegeneration Research Collection

    Type
    Collection
    ...upper and lower motor neurons in the brain and spinal cord. Over time, a loss of motor neurons leads to...opens in a new window) Research workspace on Protocols.io (Link opens in a new window) . This resource...

  7. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... construct for every application. Plus, the photophysical properties of chemical dyes provide many advantages...Organic dyes are typically both brighter and more photostable than fluorescent proteins (often up to 10-fold...

  9. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ... (2) a complementary DNA sequence, and (3) a protospacer-adjacent motif (PAM) on the opposite strand. ...this strand is edited by base editors! PAM Protospacer-Adjacent Motif A short sequence that must be ...

  10. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever...10.1016/j.cell.2014.02.001 Osuka, S., Isomura, K., Kajimoto, S., Komori, T., Nishimasu, H., Shima, T., Nureki...

  12. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ..., ABE8s resulted in 1.5-fold more editing at protospacer positions A5-A7 and 3.2-fold more editing at ...Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki...

  13. Antibodies

    Type
    Collection
    ...production process by sharing our protocols and quality control methods. Learn More Guide ...Recipient Instructions Addgene Help Center Antibody Protocols Looking for Antibody Plasmids? Browse our collection...

  14. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...change in RNA. dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...

  17. DNA Service - Cloning Grade DNA

    Type
    Collection
    ... and 260/280 ratio has been determined by spectrophotometer. Other methods of quantification may yield...or scientific support? Please see our Addgene Protocols for resources you may find helpful. Our Scientific...

  19. Mammalian RNAi Tools

    Type
    Collection
    ...Resources pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral packaging plasmids Web...

  20. mTOR Pathway

    Type
    Collection
    ...GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1 Also known as MAPKAP1; Mitogen-activated...eta PKC iota PKC theta PKC zeta Protein kinase C Protor Also known as PRR5; proline rich 5 PTEN Phosphatase...
Showing: 341 - 360 of 660 results