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Showing: 361 - 380 of 648 results

  1. Zebrafish Plasmid Collection

    Type
    Collection
    ...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...International Resource Center (ZIRC) - A US-based repository for zebrafish strains and materials and information...European Zebrafish Resource Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT...

  2. CRISPR Guide

    Type
    Collection
    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...following the amplification protocol specified by the depositing lab. After amplification, you’ll need to use ... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...CRISPR 101 eBook (3rd edition) CRISPR software and depositor protocols JOVE video reprint: Protocol for genomic...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...

  3. Cell Migration Consortium Plasmids

    Type
    Collection
    ... Addgene, which highlights CMC depositing labs and plasmids.... Depositor Collections Cell Migration Consortium...laboratories of the Cell Migration Consortium have deposited plasmids useful for cell migration research at...at Addgene. CMC investigators who have deposited plasmids include: Joan Brugge , Harvard Medical School...

  4. Plan Your Experiment

    Type
    Collection
    ...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify...Additional Resources: CRISPR protocols from Addgene depositors JOVE Video Reprint: Protocol for genomic deletions...in question (see CRISPR protocols from Addgene depositors ). Deliver Cas9 and gRNA Choose a delivery method...facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. PMID: 26066060 Zuris, J. A., Thompson...

  5. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid... Depositor Collections Brzezinski Lab CRISPR Collection Brzezinski...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...

  6. Distribution to Industry

    Type
    Collection
    ...Addgene’s deposit agreements allow Addgene to distribute plasmids on behalf of the depositing institutions...legal agreement between the recipient and the depositing institution(s). It governs the transfer of biological...entity is qualified to receive materials from our repository. All purchases are subject to Addgene’s Terms...

  7. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core...Core (Link opens in a new window) have kindly deposited a series of lentiviral and adenoviral transfer...set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases. Genes...

  8. Lentiviral Prep Service

    Type
    Collection
    ... made from plasmids that are currently in our repository. You can obtain the plasmid from us or receive...on each item's material page. Actual titers are reported with each shipment. For more details about our... that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting specific...of available viruses based on plasmids in our repository that express fluorescent proteins. ID Name Insert...

  9. TALEN Plasmids and Kits

    Type
    Collection
    ...repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains 336 pFusX...with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description Golden Gate TALEN 2.0 38142 RCIscript-GoldyTALEN... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ...

  10. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration Recombination...link to view all plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type...link to view all available plasmids from this depositor's lab. Plasmid Description Vector Type Selectable...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ...induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. Woltjen Plasmid Mouse...self-regulating and nonintegrating viral vectors. Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. Jaenisch...Cells and B Lymphocytes to Neural Stem Cells. Cell Reports. 2014 Dec 11;9(5):1673-80. Parmar Fibroblasts Astrocytes...astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1):25-36. Broccoli Fibroblasts Sensory...

  12. KLF Research Plasmids

    Type
    Collection
    ...Learn about the benefits of depositing plasmids with Addgene or email deposit@addgene.org to get started...Collections KLF Plasmids Plasmids for KLF Research KLF Depositing Labs Juan Belmonte Linzhao Cheng Jose Cibelli...

  13. NETRF

    Type
    Collection
    ...Addgene Depositors that have received funding from the Neuroendocrine Tumor Research Foundation... the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution ...

  17. Fluorescent Protein Guide

    Type
    Collection
    ...the plasmids that are available from Addgene's depositing scientists....is a Monomer not a Monomer? Addgene's plasmid repository contains a variety of fluorescent protein plasmids...the plasmids that are available from Addgene's depositing scientists. Browse Fluorescent Protein Plasmids...

  18. AAV Viral Preps

    Type
    Collection
    ...on each item's material page. Actual titers are reported with each shipment. If you can't find the prep...service. You can choose a transfer plasmid from our repository and request packaging in one of five serotypes...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster...

  19. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...celebrate Addgene without recognizing our incredible depositors and requestors. Thank you all so much for an ...

  20. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...the Addgene collection? Click here to start the deposit process and have your plasmids added to the list...addgene.org for more information and assistance with the deposit process. Want more information on the wide variety...publication or plasmid information page, or contact the depositing PI for more information on the experimental use...
Showing: 361 - 380 of 648 results