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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 402 results for: Mut

Showing: 361 - 380 of 402 results

  1. Plant Plasmids and Resources

    Type
    Collection
    ...(Link opens in a new window) for Targeted Gene Mutation in Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit...assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector with GRF4-GIF1...

  2. Optogenetics Guide

    Type
    Guide
    ...improve these natural opsins - by inducing point mutations to alter the absorption spectrum or adding trafficking... variants have been created via genetic point mutations, codon optimization, and chimeric fusion of domains...photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster kinetics but reduces photocurrent...photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/SSFO Step function...function Opsins and Stabilized Step Function Opsins. Mutations at C128 and D156 of CrChR delay the closing of...Chlamydomonas noctigama . ChrimsonR carries K176R mutation. 590 PsChR2 High-efficiency blue-shifted channelrhodopsin... C1V1 ChR1-VChR1 chimera with E122T and E162T mutations 540 Channelrhodopsins: chloride channels iChloC...

  3. Plan Your Experiment

    Type
    Guide
    ...(knockout)? Generate a specific mutant allele of a gene (point mutant)? Increase or decrease expression...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the...many different types of edits, including point mutations, base changes, and large insertions or deletions...) Generate specific genomic edits, like point mutations or small tag insertions, by copying from a DNA...Generate targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA) Prime... alternative splicing, and an early frameshift mutation is more likely to result in a non-functional protein...base editing , or prime editing experiments, most mutated alleles will not contain the desired edit. Even...

  4. Zebrafish Plasmid Collection

    Type
    Collection
    ...Keith Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9... typically relies on genetic markers, somatic mutations, or labeling with fluorescent proteins to visualize...

  5. Ras Pathway

    Type
    Collection
    ...regulating the growth of the cell, however when mutations occur in the Ras gene it can be stuck in a constitutively...PubMed PMID: 17428555 . RAS signaling pathways, mutations and their role in colorectal cancer. Zenonos K...

  6. CRISPR Plasmids - Tagging

    Type
    Collection
    ...systematic and scalable method to create marker-free mutations, insertions, and deletions at any locus in C. ... 10-day protocol, generates “clean” homozygous mutants with no co-integrated markers or footprints, and...

  7. Retrograde AAV viral preps

    Type
    Collection
    ...Norepinephrine sensor Yulong Li 123310 pAAV-hSyn-GRAB_NEmut Syn GRAB-NEmut control norepinephrine sensor (does not...

  8. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...with the pooled library to create a population of mutant cells that are then screened for a phenotype of...Human Wei 3rd Varies 12,472 pairs HR700_TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human Stiewe...

  9. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... create and test capsid libraries with random mutations or insertions in the Cap region. Several methods...immune response, and the low risk of insertional mutagenesis. Several AAV-based gene therapies have been approved...chromosome, which reduces the risk of insertional mutagenesis. However, random integration events can occur...host organism, leading to disease. Insertional mutagenesis Random insertion of DNA elements into the genome...material into cells. Replication-competent rAAV Mutated rAAV vector that has the ability to replicate and...

  11. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...sgUPRT 54467 Other/Toxoplasma gondii none, Q5 mutagenesis yes, cut S. pyogenes Sibley pU6-Universal 52694...Gateway recombination. Qi Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs...

  12. Immunology Research Plasmids and Resources

    Type
    Collection
    ...arginine-rich, mutated in early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early..., DC-SIGN, DC-SIGN1, MGC129965 CD4 CD4 molecule CD4mut CD74 CD74 molecule, major histocompatibility complex...FLJ79544, FLJ94613, MGC138597, T3G CD4 CD4 molecule CD4mut CD40LG CD40 ligand CD154, CD40L, HIGM1, IGM, IMD3...consists of 182 wild-type and 19 well-characterized mutant ORFs that represent over 25 cellular signaling ...

  13. Lentiviral Vector Guide

    Type
    Guide
    ...harnessed for stable delivery of various genes, mutations, or treatments into cells, and has been widely... the laboratory, and many have been removed or mutated for increased safety. Figure 1: Wild-type lentivirus...biological processes by surveying a pool of genetic mutants for various phenotypes. These screens involve hundreds...used to create transgenic mice with heritable mutations or transgene expression. Popular uses include ...

  14. Chemogenetics Guide

    Type
    Guide
    ...? blog! Studies of GPCRs led to development of mutated receptors that while unresponsive to endogenous...PSEMs). PSAM domains were first named for the mutations that allowed them to respond to their cognate ...anti-smoking drug varenicline. This PSAM carries mutations at L131G, Q139L, and Y217F and is termed PSAM4...

  15. Adenovirus Guide

    Type
    Guide
    ...highly reduces the potential for insertional mutagenesis and the risk of oncogenesis. However, random ... signals of infection or damage. Insertional mutagenesis Random insertion of DNA elements into the genome... cells. Replication-competent adenovirus (RCA) Mutated adenoviral vector that has the ability to replicate...

  16. Browse

    Type
    Collection
    ...Plasmids Plasmid Gene/Insert Vector Type Backbone Mutations Tags Industry PI...
Showing: 361 - 380 of 402 results